Genomic Constitution and Expression of Disease Resistance in Agropyron cristatum * Durum Wheat Derivatives

Soliman, Mahmoud H.; Cabrera, Adoración; Sillero, Josefina C.; Rubiales, Diego

doi:10.1270/jsbbs.57.17

Cited by 9 publications

(6 citation statements)

References 13 publications

(14 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the crossability of diploid A. cristatum with wheat is low due to the disequilibrium in the endosperm balance number in the hybrid seeds (Chen et al 1989), while hybridization between wheat and tetraploid A. cristatum can be easily done (Chen et al 1989; Martín et al 1999). As the crosses between wheat and hexaploid A. cristatum have not been reported yet, the tetraploid form is the most widely used crossing partner in the wheat-crested wheatgrass introgression breeding programs (Chen et al 1989, 1994; Li et al 1997, 1998, 2016; Soliman et al 2007; Han et al 2014; Ochoa et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Uncovering homeologous relationships between tetraploid Agropyron cristatum and bread wheat genomes using COS markers

et al. 2019

Self Cite

View full text Add to dashboard Cite

Key messageUsing COS markers, the study reveals homeologous relationships between tetraploid Agropyron cristatum and bread wheat to support alien introgression breeding of wheat.AbstractCrested wheatgrass (Agropyron cristatum L. Gaertn.) is a wild relative of wheat that possesses many genes that are potentially useful in wheat improvement. The species comprises a complex of diploid, tetraploid and hexaploid forms. In this study, wheat–A. cristatum chromosome, telosome and translocation lines were used to characterize syntenic relationships between tetraploid A. cristatum and bread wheat. Prior to mapping COS markers, the cytogenetic stock lines were characterized for fertility and by FISH and GISH for karyotype stability. Out of 328 COS markers selected for the study, 279 consistently amplified products in tetraploid A. cristatum, and, out of these, 139 were polymorphic between tetraploid crested wheatgrass and wheat. Sixty-nine markers were found to be suitable for the detection of tetraploid A. cristatum chromosomes 1P–6P in wheat, ranging from 6 to 17 markers per chromosome. BLASTn of the source ESTs resulted in significant hits for 67 markers on the wheat pseudomolecules. Generally, COS markers of the same homeologous group were detected on similar arms in both Agropyron and wheat. However, some intragenomic duplications and chromosome rearrangements were detected in tetraploid A. cristatum. These results provide new insights into the structure and evolution of the tetraploid A. cristatum genome and will facilitate the exploitation of the wild species for introgression breeding of bread wheat.Electronic supplementary materialThe online version of this article (10.1007/s00122-019-03394-1) contains supplementary material, which is available to authorized users.

show abstract

Section: Introductionmentioning

confidence: 99%

Uncovering homeologous relationships between tetraploid Agropyron cristatum and bread wheat genomes using COS markers

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…durum Desf.) (Soliman et al 2007). The objectives of the present work were to study the effect of an A. cristatum-wheat compensating robertsonian translocation line on leaf rust resistance and to conduct molecular and cytological characterization of that line.…”

Section: Introductionmentioning

confidence: 99%

Molecular and cytogenetic characterization of a common wheat-Agropyron cristatum chromosome translocation conferring resistance to leaf rust

et al. 2014

Self Cite

View full text Add to dashboard Cite

Crested wheatgrass (Agropyron cristatum L. Gaertn.) is a perennial species of economic importance as forage that also displays potentially valuable traits for wheat improvement trough intergeneric hybridization. In order to incorporate resistance genes from A. cristatum against wheat leaf rust (Puccinia triticina Erikss.) into common wheat (Triticum aestivum L.) a breeding programme was carried out by crossing and backcrossing the self-fertile amphiploid AABBDDPP (2n = 8x = 56) with T. aestivum (2n = 6x = 42; AABBDD). The AABBDDPP amphiploid was previously obtained by crossing tetraploid wheat (Triticum turgidum L. conv. durum Desf. 2n = 4x = 28; AABB) with a self-fertile allotetraploid (2n = 4x = 28; DDPP) between diploid wheat (Aegilops tauschii Coss.) and crested wheatgrass (A. cristatum). After three backcrosses a fertile stable line (named TH4) was obtained with 42 chromosomes. The fluorescence in situ hybridization and GISH analysis confirmed that TH4 carries a compensating robertsonian translocation involving the long arm of wheat chromosome 1B and the short arm of an unidentified A. cristatum chromosome. Specific molecular markers from A. cristatum also demonstrated the presence of chromatin from this species in the TH4 line. Macroscopic and microscopic observations indicated that the A. cristatum fragment that has been transferred to common wheat contributed a substantial level of partial resistance to leaf rust. The A. cristatum translocation line in bread wheat makes the disease resistance gene(s) from A. cristatum accessible for wheat breeding programmes.

show abstract

“…For example, hybrids between Triticum and A. cristatum have been obtained to introduce traits from A. cristatum into Triticum [15]. Both durum and common wheat A. cristatum introgression lines have also been developed [8,14,18,19] and they could be a useful source of agronomic traits, such as disease resistance [9][10][11], abiotic stresses [12,13], thousand grain weight [14], and grain quality [36].…”

Section: Discussionmentioning

confidence: 99%

“…Hybrids and fertile amphiploids between Triticum and A. cristatum have been successfully obtained [15][16][17][18] with the aim to develop wheat-A. cristatum introgressions [8,14,19].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Chromosome Associations during Early Meiosis in Wheat Lines Carrying Chromosome Introgressions from Agropyron cristatum

Prieto

Palomino

Cifuentes

et al. 2021

Plants

Self Cite

View full text Add to dashboard Cite

Crested wheatgrass (Agropyron cristatum L. Gaertn., genome P), included in the Triticeae tribe (family Poaceae), is one of the most important grasses in temperate regions. It has been valued as a donor of important agronomic traits for wheat improvement, including tolerance to cold, drought, and high salinity, as well as resistance to leaf rust, stripe rust, and powdery mildew. For successful incorporation of beneficial alleles into wheat, it is essential that recombination between wheat and A. cristatum chromosomes occurs. In this work, we analysed chromosome associations during meiosis in wheat lines carrying chromosome introgressions from A. cristatum chromosomes 5P and 6P in the presence and absence of Ph1 locus using fluorescence in situ hybridisation. The results showed that the Ph1 locus does not affect chromosome associations between A. cristatum and wheat chromosomes because there were no interspecific chromosome associations; therefore, no recombination between chromosomes from wheat and Agropyron were observed in the absence of the Ph1 locus. The 5P and 6P A. cristatum chromosomes do not have a suppressor effect on the Ph1 locus. Wheat univalents in metaphase I suggest that Agropyron chromosomes might carry genes having a role in wheat homologous chromosome associations. Putative effect of the Agropyron genes on wheat chromosome associations does not interact with the Ph1 locus.

show abstract

Genomic Constitution and Expression of Disease Resistance in Agropyron cristatum * Durum Wheat Derivatives

Cited by 9 publications

References 13 publications

Uncovering homeologous relationships between tetraploid Agropyron cristatum and bread wheat genomes using COS markers

Uncovering homeologous relationships between tetraploid Agropyron cristatum and bread wheat genomes using COS markers

Molecular and cytogenetic characterization of a common wheat-Agropyron cristatum chromosome translocation conferring resistance to leaf rust

Analysis of Chromosome Associations during Early Meiosis in Wheat Lines Carrying Chromosome Introgressions from Agropyron cristatum

Contact Info

Product

Resources

About