Genomic cloning, expression, and single nucleotide polymorphism association analysis of the insulin-like androgenic gland hormone gene in the oriental river prawn (Macrobrachium nipponense)

Abstract

ABSTRACT. Increasing evidence suggests that the insulin-like androgenic gland hormone (IAG) gene plays an important role in male sexual differentiation, metabolism, and growth in crustaceans. In the present study, we isolated the full-length genome sequence of IAG by genome walking based on the cDNA sequence in Macrobrachium nipponense. Four novel single nucleotide polymorphisms (SNPs) were studied, including 509G>T, 529G>T, 590A>T in intron 1, and 2226A>G in intron 2. The association of genetic variation with growth traits [body length (BL) and body weight (BW)] was analyzed. Individuals with GG geno- 5911 SNPs of the IAG gene in Macrobrachium nipponense©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 5910-5921 (2015) type at locus 2226A>G maintained higher mean BL (P < 0.01) and BW (P < 0.05) than AA and GA individuals. These results suggest that IAG SNPs may be useful molecular markers for selecting growth traits in M. nipponense.

Comparative Transcriptome Analysis Reveals Related Regulatory Mechanisms of Androgenic Gland inEriocheir sinensis

“…FOXL2 negatively regulates the VTG synthesis at developmental stage. FOXL2 downregulates VTG's expression by binding DDX20 in the regulation of follicular cell apoptosis and repress the synthesis of VTG when bound with FTZ-F1 at the mature stage [ 18 , 34 , 35 ]. According to our research, during the process of testis development, FOXL2 had no obvious change, and FTZ-F1 gene was downregulated.…”

Two Short Repeats in the 5′ Untranslated Region of Insulin-like Androgenic Gland Factor in Procambarus clarkii (PcIAG) That Regulate PcIAG Expression

“…It could provide a theoretical basis for understanding the sex differentiation and molecular regulation mechanism of P. clarkii . At present, the IAG DNA sequences of M. nipponense , Scylla paramamosain , P. fallax, and F. chinensis have been obtained [ 31 , 32 , 33 , 34 ]. The IAG DNA sequence of P. clarkii was comprised of four exons and three introns, similar to the IAG sequence of P. fallax and S. Paramamosain .…”

A sex‐reversing factor: insulin‐like androgenic gland hormone in decapods

“…The IAG genome sequence was comprised of four exons and three introns in the mub crab Scylla paramamosain and the P. fallax (Huang et al 2014;Levy et al 2017;Fig. 2a), and of five exons and three introns in the Chinese shrimp Fenneropenaeus chinensis and the oriental river prawn Macrobrachium nipponense (Li et al 2012;Li et al 2015a;Li et al 2015c;Fig. 2b).…”

“…Figure2IAG gene structure (Modified fromLi et al 2015c). (a) The IAG gene structure contained four exons and three introns(Huang et al 2014;Levy et al 2017).…”

“…(a) The IAG gene structure contained four exons and three introns(Huang et al 2014;Levy et al 2017). (b) The IAG gene structure contained five exons and four introns(Li et al 2012;Li et al 2015a;Li et al 2015c). Green boxes represent exon.…”