Genomic Characterizations of Six Pigeon Paramyxovirus Type 1 Viruses Isolated from Live Bird Markets in China during 2011 to 2013

Wang, Jingjing; Liu, Hualei; Liu, Wei; Zheng, Dong; Zhao, Yanyan; Li, Yin; Wang, Yingli; Ge, Shengxiang; Liu, Yan; Zuo, Yunjuan; Song, Yu; Wang, Zhiliang

doi:10.1371/journal.pone.0124261

Cited by 46 publications

(36 citation statements)

References 49 publications

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“…The cross-reactivity HI assay showed that the R value between the GZ08 strain and the commonly used vaccine LaSota was 0.59, indicating a minor antigenic difference between LaSota and GZ08. The results were similar to those of previous research [24,34]. A large number of reports confirmed that antigen matching between vaccine and epidemic strains could increase the effectiveness of vaccines against epidemic strains [51][52][53][54].…”

Section: Discussionsupporting

confidence: 90%

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Evolutionary Dynamics and Age-Dependent Pathogenesis of Sub-Genotype VI.2.1.1.2.2 PPMV-1 in Pigeons

Xie

Chen

Zhang

et al. 2020

Viruses

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Pigeon paramyxovirus type 1 (PPMV-1) infection causes high morbidity in pigeons, resulting in a significant burden to the poultry industry. In this study, we isolated three PPMV-1 strains from diseased pigeons collected in Guangdong Province, South China, from June 2017 to April 2019. Genetic analysis revealed that these three PPMV-1 strains and most of the PPMV-1 strains isolated from China after 2011 were clustered into sub-genotype VI.2.1.1.2.2. Our Bayesian analysis revealed that the VI.2.1.1.2.2 viruses might have originated in Europe. Phylogeographic analyses revealed that East and South China might have played a key role in seeding the VI.2.1.1.2.2 PPMV-1 epidemic in China. To characterize the effect of age at infection on the outcome of PPMV-1 infection in pigeons, we investigated the pathogenesis and transmission of the pigeon/Guangdong/GZ08/2017 (GZ08) virus in 3-, 6-, and 12-week-old pigeons. Two of six 12-week-old pigeons inoculated with GZ08 survived, and all of the 3- and 6-week-pigeons inoculated with GZ08 died. Moreover, the GZ08 virus could be transmitted to 3-, 6-, and 12-week-old naïve contact pigeons. The lethality of the GZ08 virus through contact with 3-, 6-, and 12-week-old pigeons was 100%, 66.7%, and 0%, respectively, suggesting that the transmissibility of the GZ08 virus was stronger in young pigeons. These findings demonstrated that East and South China was the epicenter for dissemination of VI.2.1.1.2.2 PPMV-1, and age at infection has an impact on the outcome of PPMV-1 infection in pigeons.

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Section: Discussionsupporting

confidence: 90%

“…The cross-reactivity HI assay between the commonly used vaccine LaSota and the PPMV-1 strain, GZ08, was conducted as described previously [34]. Briefly, 3-week-old pigeons were intramuscularly injected with inactivated oil-emulsified LaSota or GZ08 virus.…”

Section: Cross-reactivity Hi Assaymentioning

confidence: 99%

Evolutionary Dynamics and Age-Dependent Pathogenesis of Sub-Genotype VI.2.1.1.2.2 PPMV-1 in Pigeons

Xie

Chen

Zhang

et al. 2020

Viruses

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“…Since the neutralization antibodies raised against the F protein define the protection status of the host, these modifications in the F protein explain the potential of immune escape and lack of sterile immunity caused by currently available vaccines. Moreover, the substitutions particularly in the fusion peptide, hydrophobic regions and transmembrane region of the F protein and neutralizing epitopes of the HN protein could result in altered fusion activity and neutralizing escape variants [14,15,37]. Taken together, nucleotide and subsequent amino acid substitutions explained the evolving nature of RNA viruses including the AAvV-1 however warrant future investigation to delineate the importance of these mutations in the pathobiology of AAvV-1 [16,20,36].…”

Section: Discussionmentioning

confidence: 99%

“…The egg lethal dose 50 (ELD50) was calculated by Reed and Muench method [29]. Hemagglutination inhibition (HI) assay was performed as previously described [26,37] using four hemagglutinating units for LaSota, NDV/Cattle egret/Egypt/SDU-1/2016 and NDV/Teal/Egypt/SDU-4/2016 and antisera collected from SPF chickens vaccinated with LaSota (Genotype II) live attenuated and commercial killed vaccine (Genotype VII).…”

Section: Sampling History Virus Isolation and Biological Characterizmentioning

confidence: 99%

Biological characterization of wild-bird-origin avian avulavirus 1 and efficacy of currently applied vaccines against potential infection in commercial poultry

et al. 2018

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Newcastle disease virus (NDV), the type member of the species Avian avulavirus 1 (formerly known as avian paramyxovirus serotype 1), causes a highly contagious and economically important disease in a myriad of avian species around the globe. While extensive vaccination programs have been implemented in ND-endemic countries, the disease is continuously spreading in commercial, backyard, and wild captive poultry. In order to investigate the evolution of the virus and assess the efficiency of the vaccine regimens that are currently being applied in commercial poultry, four wild-bird-origin NDV strains were characterized biologically, based on mean death time and intracerebral pathogenicity index, and genetically, based on the cleavage motif (RRQKRF) in the fusion (F) protein. Based on these features, all of the isolates were characterized as velogenic strains of NDV. Phylogenetic analysis based on the complete genome sequence revealed clustering of these isolates within class II, genotype VII. This class of NDV remains the predominant genotype in the Egyptian poultry industry, as well as in those of many Asian and African countries. To investigate the potential of these wild-bird-origin NDV isolates to cause infection in domesticated poultry and to assess the efficacy of currently available vaccines for protection of commercial poultry, an extensive animal challenge experiment was performed. Cumulative clinicopathological and immunological investigations of virus-challenged chickens indicate that these isolates can potentially be transmitted between chicken and cause systemic infections, and the currently applied vaccines are unable to prevent clinical disease and virus shedding. Taken together, the data represent a comprehensive evaluation of the ability of Egyptian wild-bird-origin NDV strains to cause infection in commercial poultry and highlights the need for a continuous and large-scale surveillance as well as revised vaccine approaches. These integrated and multifaceted strategies would be crucial in any efforts to control and eradicate the disease globally.

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“…PPMV-1 was introduced to Hong Kong in 1985, subsequently spread throughout China, and now poses a serious threat to the Chinese pigeon breeding industry and wild birds population [12]. The virus is now one of the most destructive pathogens for pigeons in the country, also affecting pigeon farms with increased frequency (Awu et al 2015; Guo et al 2013;Ren et al 2016;Wei et al 2018).…”

Section: Introductionmentioning

confidence: 99%

Complete Genome Sequencing, Molecular Epidemiological, and Pathogenicity Analysis of Pigeon Paramyxoviruses Type 1 Isolated in Guangxi, China during 2012–2018

Dimitrov³

et al. 2020

Viruses

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Newcastle disease is an important poultry disease that also affects Columbiform birds. The viruses adapted to pigeons and doves are referred to as pigeon paramyxoviruses 1 (PPMV-1). PPMV-1 are frequently isolated from pigeons worldwide and have the potential to cause disease in chickens. The complete genomes of 18 PPMV-1 isolated in China during 2012–2018 were sequenced by next-generation sequencing (NGS). Comprehensive phylogenetic analyses showed that five of the viruses belong to sub-genotype VI1.2.1.1.2.1 and 13 isolates belong to sub-genotype VI.2.1.1.2.2. The results demonstrate that these sub-genotypes have been predominant in China during the last decade. The viruses of these sub-genotypes have been independently maintained and continuously evolved for over 20 years, and differ significantly from those causing outbreaks worldwide during the 1980s to 2010s. The viral reservoir remains unknown and possibilities of the viruses being maintained in both pigeon farms and wild bird populations are viable. In vivo characterization of the isolates’ pathogenicity estimated mean death times between 62 and 114 h and intracerebral pathogenicity indices between 0.00 and 0.63. Cross-reactivity testing showed minor antigenic differences between the studied viruses and the genotype II LaSota vaccine. These data will facilitate PPMV-1 epidemiology studies, vaccine development, and control of Newcastle disease in pigeons and poultry.

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Genomic Characterizations of Six Pigeon Paramyxovirus Type 1 Viruses Isolated from Live Bird Markets in China during 2011 to 2013

Cited by 46 publications

References 49 publications

Evolutionary Dynamics and Age-Dependent Pathogenesis of Sub-Genotype VI.2.1.1.2.2 PPMV-1 in Pigeons

Evolutionary Dynamics and Age-Dependent Pathogenesis of Sub-Genotype VI.2.1.1.2.2 PPMV-1 in Pigeons

Biological characterization of wild-bird-origin avian avulavirus 1 and efficacy of currently applied vaccines against potential infection in commercial poultry

Complete Genome Sequencing, Molecular Epidemiological, and Pathogenicity Analysis of Pigeon Paramyxoviruses Type 1 Isolated in Guangxi, China during 2012–2018

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