Genomic characterization of putative allergen genes in peach/almond and their synteny with apple

Chen, Lin; Zhang, Shuiming; Illa, Eudald; Song, Lijuan; Wu, Shihua; Howad, Werner; Arús, Pere; Weg, Eric van de; Chen, Kunsong; Gao, Zhongshan

doi:10.1186/1471-2164-9-543

Cited by 65 publications

(74 citation statements)

References 38 publications

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“…Amongst the 12 putative genes we mapped, the two allergic genes, LTP1(or putative allergen Pru du 3.01) and Pru1 (prunin1), have been mapped in Prunus maps previously (Viruel et al 1995;Joobeur et al 1998;Chen et al 2008). The reported locations on linkage groups 6 and 8, respectively, are conWrmed by our mapping results.…”

Section: Discussionsupporting

confidence: 85%

Mapping SNP-anchored genes using high-resolution melting analysis in almond

Tavassolian

Rabiei

et al. 2009

Mol Genet Genomics

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Peach and almond have been considered as model species for the family Rosaceae and other woody plants. Consequently, mapping and characterisation of genes in these species has important implications. High-resolution melting (HRM) analysis is a recent development in the detection of SNPs and other markers, and proved to be an efficient and cost-effective approach. In this study, we aimed to map genes corresponding to known proteins in other species using the HRM approach. Prunus unigenes were searched and compared with known proteins in the public databases. We developed single-nucleotide polymorphism (SNP) markers, polymorphic in a mapping population produced from a cross between the cloned cultivars Nonpareil and Lauranne. A total of 12 SNP-anchored putative genes were genotyped in the population using HRM, and mapped to an existing linkage map. These genes were mapped on six linkage groups, and the predicted proteins were compared to putative orthologs in other species. Amongst those genes, four were abiotic stress-responsive genes, which can provide a starting point for construction of an abiotic resistance map. Two allergy and detoxification related genes, respectively, were also mapped and analysed. Most of the investigated genes had high similarities to sequences from closely related species such as apricot, apple and other eudicots, and these are putatively orthologous. In addition, it was shown that HRM can be an effective means of genotyping populations for the purpose of constructing a linkage map. Our work provides basic genomic information for the 12 genes, which can be used for further genetic and functional studies.

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Section: Discussionsupporting

confidence: 85%

Mapping SNP-anchored genes using high-resolution melting analysis in almond

Tavassolian

Rabiei

et al. 2009

Mol Genet Genomics

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“…Recent data indicated that a Pru p 1.01 isoform, named Pru p 1.06D according to its sequence identity (Gao et al, 2005;Chen et al, 2008), is one of the most abundantly expressed genes among the ripe peach mesocarp transcriptome (Trainotti et al, 2006). Pru p 1.06D shows high sequence identity (73%) to Pru p Figure 5.…”

Section: Discussionmentioning

confidence: 99%

The RNA Hydrolysis and the Cytokinin Binding Activities of PR-10 Proteins Are Differently Performed by Two Isoforms of the Pru p 1 Peach Major Allergen and Are Possibly Functionally Related

et al. 2009

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PR-10 proteins are a family of pathogenesis-related (PR) allergenic proteins playing multifunctional roles. The peach (Prunus persica) major allergen, Pru p 1.01, and its isoform, Pru p 1.06D, were found highly expressed in the fruit skin at the pit hardening stage, when fruits transiently lose their susceptibility to the fungal pathogen Monilinia spp. To investigate the possible role of the two Pru p 1 isoforms in plant defense, the recombinant proteins were expressed in Escherichia coli and purified. Light scattering experiments and circular dichroism spectroscopy showed that both proteins are monomers in solution with secondary structures typical of PR-10 proteins. Even though the proteins do not display direct antimicrobial activity, they both act as RNases, a function possibly related to defense. The RNase activity is different for the two proteins, and only that of Pru p 1.01 is affected in the presence of the cytokinin zeatin, suggesting a physiological correlation between Pru p 1.01 ligand binding and enzymatic activity. The binding of zeatin to Pru p 1.01 was evaluated using isothermal titration calorimetry, which provided information on the stoichiometry and on the thermodynamic parameters of the interaction. The structural architecture of Pru p 1.01 and Pru p 1.06D was obtained by homology modeling, and the differences in the binding pockets, possibly accounting for the observed difference in binding activity, were evaluated.

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“…Other sequences within the same cluster include apple Md-PR5 and pear Ppy-TLP with sequence similarities of 91 and 92%, respectively. Pp-TLP3 is probably a homolog of Pru p 2.04 (99% similarity), the putative allergene from (P. dulcis 9 P. persica) (Chen et al 2008). Pp-TLP3 is located within the same cluster of Brassica rapa (Br-TLP).…”

Section: Characterization Of Peach Pr1 and Tlp Proteinsmentioning

confidence: 99%

“…8). Regardless of many putative elements identified in the PlantPAN database (Chen et al 2008), only those with a previously demonstrated role in disease resistance or hormone signaling were considered. The binding sites for transcription factors MYC2 (JA signaling), ERF (JA/ET signalling), WRKY (SA signalling) and TGA (SA signaling) were recognized in both plus and minus DNA-strands.…”

Section: The Identification Of Cis-acting Elements In Tlp Promotersmentioning

confidence: 99%

Molecular characterization of peach PR genes and their induction kinetics in response to bacterial infection and signaling molecules

2011

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'Venture' and 'BabyGold 5' are two peach cultivars with a demonstrated resistance and susceptibility, respectively, to bacterial spot disease caused by Xanthomonas campestris pv. pruni (Xcp). To explore the differences between these cultivars at the molecular level, two PR1 (Pp-PR1a, Pp-PR1b) and three PR5 (Pp-TLP1, Pp-TLP2 and Pp-TLP3) genes were isolated from peach (Prunus persica L.) and investigated by in silico and in situ approaches. The analysis of gene expression by qRT-PCR indicated that all PR genes, except Pp-PR1a, were induced to a significantly higher degree in the resistant cultivar. In response to signaling molecules, Pp-PR1a was induced chiefly by SA treatment, while other PR genes were induced mainly by ethephon or MeJA treatments. The induction of the same set of PR genes in response to bacterial infection, MeJA or ethephon suggests the involvement of jasmonic acid (JA)/ethylene (ET)-signaling pathways in mediating resistance against Xcp, which is consistent with the potential hemibiotrophic nature of this bacterium. The identification of binding sites for ERF and MYC2 transcription factors in the promoter of Pp-TLP1 and Pp-TLP2 genes further supported the role of JA/ET pathways in the transcription regulation of these genes. The role of stomata in defense against Xcp was also investigated by measuring stomatal apertures in both 'Venture' and 'BabyGold 5' leaves after 1 and 3 HPI. While most stomata closed in both cultivars within 1 HPI, stomata reopened again at 3 HPI with a higher percentage recorded for 'BabyGold 5', suggesting a potential role of stomata in the susceptibility of this cultivar.

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Genomic characterization of putative allergen genes in peach/almond and their synteny with apple

Cited by 65 publications

References 38 publications

Mapping SNP-anchored genes using high-resolution melting analysis in almond

Mapping SNP-anchored genes using high-resolution melting analysis in almond

The RNA Hydrolysis and the Cytokinin Binding Activities of PR-10 Proteins Are Differently Performed by Two Isoforms of the Pru p 1 Peach Major Allergen and Are Possibly Functionally Related

Molecular characterization of peach PR genes and their induction kinetics in response to bacterial infection and signaling molecules

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