Genomic characterization of novel IncFII-type multidrug resistant plasmids p0716-KPC and p12181-KPC from Klebsiella pneumoniae

Feng, Jiao; Yin, Zhe; Zhao, Qian; Zhao, Yachao; Zhang, Defu; Jiang, Xiaoyuan; Wu, Weili; Chen, Weijun; Wang, Hui; Song, Yajun; Tong, Yigang; Wang, Jinglin; Li, Yanjun; Zhou, Dongsheng

doi:10.1038/s41598-017-06283-z

Cited by 22 publications

(22 citation statements)

References 37 publications

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“…IncFII k plasmid, a member of the divergent IncFII replicon plasmids, played a significant role in restoring and transferring the bla KPC gene in K. pneumoniae ( Feng et al, 2017 ; Wang et al, 2017 ; Bi et al, 2018 ; Fu et al, 2019 ), which was also reported sporadically to carry MBL-encoding genes, such as bla NDM ( Sugawara et al, 2019 ). The plasmid pIMP1572 identified in this study is different from previously reported bla IMP -harboring plasmids that belonged to incompatibility groups IncL/M, A/C, HI2, and IncN ( Carattoli, 2009 ; Dolejska et al, 2016 ; Wang et al, 2017 ) and represents the first report of IncFII k plasmid carrying bla IMP .…”

Section: Resultsmentioning

confidence: 99%

Molecular Characterization of an IncFIIk Plasmid Co-harboring blaIMP–26 and tet(A) Variant in a Clinical Klebsiella pneumoniae Isolate

Yao

Cheng

et al. 2020

Front. Microbiol.

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Carbapenems and tigecycline are two important classes of antimicrobial agents to treat the infections caused by Enterobacterales. Here, we reported a plasmid carrying both bla IMP−26 and tet(A) variant in clinical Klebsiella pneumoniae KP-1572. MIC results showed that K. pneumonia KP-1572 was resistant to a wide range of antimicrobials. The bla IMP−26 and tet(A) variant were located on an identical plasmid, which was indicated by S1-PFGE and southern blotting hybridization and can be successfully transferred by electroporation. Whole-plasmid sequencing and analysis revealed that a 142,993-bp-sized plasmid, designated pIMP1572, contains an IncFII k backbone and a variable region harboring bla IMP−26 and tet(A) variant. The plasmid pIMP1572 was apparently originated from a tet(A)-carrying IncFII k plasmid but with a deletion length of 6,216-bp and a multiple drug resistance region (MDRR) insertion of 25,259 bp. The plasmid pIMP1572 in the present study represents the first report of the IncFII k plasmid co-carrying bla IMP and tet(A) variant, which should be monitored.

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Section: Resultsmentioning

confidence: 99%

Molecular Characterization of an IncFIIk Plasmid Co-harboring blaIMP–26 and tet(A) Variant in a Clinical Klebsiella pneumoniae Isolate

Yao

Cheng

et al. 2020

Front. Microbiol.

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“…In China, the first case of bla KPC was found in Hangzhou, which was isolated from one elderly patient’s sputum in 2004 [ 12 ]. Currently, bla KPC has been reported in many regions of China, including Beijing, Zhejiang, Taiwan, and Sichuan [ 13 – 16 ]. To the best of our knowledge, this article is the first report on the prevalence of CNSE in the eastern part of Heilongjiang province.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization and epidemiology of carbapenem non-susceptible Enterobacteriaceae isolated from the Eastern region of Heilongjiang Province, China

Gong

Zhang

et al. 2018

BMC Infect Dis

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BackgroundThe aim of this study was to elucidate the molecular epidemiology of carbapenem non-susceptible Enterobacteriaceae(CNSE) isolated in the Eastern region of Heilongjiang Province, China, and the mechanism of carbapenem resistance.MethodsA total of 53 CNSE isolates were collected in a grade-3 hospital in Heilongjiang province. Sensitivity to antibiotics was determined using the VITEK-2 Compact automatic system. The modified Hodge test (MHT) and modified carbapenem inactivation test (mCIM) were performed for phenotypic identification. Beta-lactamases gene were detected by Polymerase chain reaction(PCR) and DNA sequencing. The transfer of blaNDM and blaKPC was investigated through conjugation experiment. The clinical data of patients were retrospectively reviewed. Homology of Carbapenem-resistant Klebsiella pneumoniae(CRKP) was conducted by multilocus sequence typing (MLST).ResultsCNSE were highly resistant to the majority of antimicrobial agents. The resistance rate was 100% for first, third, fourth generation cephalosporins and enzyme inhibitor compounds. Gentamicin and tobramycin recorded a resistance rate higher than 80%. Less than 30% resistance was detected for amikacin and levofloxacin. Among CNSE 52(98.1%) and 48(90.6%) of CNSE were positive for mCIM and MHT respectively. There were 42 positive blaKPC genes, three blaNDM-1 genes, three blaNDM-5 genes, one blaNDM-7 gene, and six blaIMP-4 genes. Most isolates harbored multiple drug resistance gene, especially as related to extended-spectrum-β-lactamases, blaSHV, blaTEM and blaCTX-M-15 genes.The resistant gene was transferred into recipient Escherichia coli J53 through conjugation in 21.3% (10/47) of the strains. MLST revealed that ST76 (n = 36) was the most predominant clone, followed by ST896, ST323 and ST11. A new one ST 2946 was identity by this study.ConclusionThe carbapenem resistance phenomenon is alarming and blaKPC-2 is the main resistant gene of CNSE in our hospital. This is the first report of an outbreak caused by blaKPC-2 positive K. pneumoniae ST76 in the Eastern region of Heilongjiang Province, China. Relevant departments should implement infection control and prevention measures to avoid further dissemination of the multi drug-resistant bacteria (MDR).

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“…IncFII plasmids are usually low copy number plasmids with a narrow host range and circulated mainly among Enterobacteriaceae species 3. Due to significant variations at nucleotide and amino acid levels of backbone sequences, Inc-FII plasmids can be divided into multiple subgroups, namely IncFII Y , IncFII K , IncFII pHN7A8 , and IncFII p0716-KPC , represented by pKOX_NDM1 (GenBank accession number JQ314407),4 pKPHS2 (GenBank accession number CP003224),5 pHN7A8 (GenBank accession number JN232517), and p0716-KPC (GenBank accession number KY270849),6,7 respectively. A single replicon IncFII pHN7A8 , three replicons IncFII K , IncFIB, and IncR, and a single replicon IncFII Y can be found in pHN7A8, pKPHS2, and pKOX_NDM1, respectively.…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of KPC-2-encoding chimera plasmids with multi-replicon IncR:IncpA1763-KPC:IncN1 or IncFIIpHN7A8:IncpA1763-KPC: IncN1

Shen²,

et al. 2019

IDR

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BackgroundIncR, IncFII, IncpA1763-KPC, and IncN1 plasmids have been increasingly found among Enterobacteriaceae species, but plasmids with hybrid structures derived from the above-mentioned incompatibility groups have not yet been described.MethodsPlasmids p721005-KPC, p504051-KPC, and pA3295-KPC were fully sequenced and compared with previously sequenced related plasmids pHN84KPC (IncR), pKPHS2 (IncFIIK), pKOX_NDM1 (IncFIIY), pHN7A8 (IncFIIpHN7A8), and R46 (IncN1).ResultsThe backbone of p721005-KPC/p504051-KPC was a hybrid of the entire 10-kb IncR-type backbone from pHN84KPC, the entire 64.3-kb IncFIIK-type maintenance, and conjugal transfer regions from pKPHS2, a 15.5-kb IncFIIY-type maintenance region from pKOX_NDM1 and a 5.6-kb IncpA1763-KPC-type backbone region from pA1763-KPC, and it contained a primary IncR replicon and two auxiliary IncpA1763-KPC and IncN1 replicons. The backbone of pA3295-KPC was a hybrid of a 7.2-kb IncFIIpHN7A8-type backbone region from pHN7A8, the almost entire 33.3-kb IncN1-type maintenance and conjugal transfer regions highly similar to R46, a 26.2-kb IncFIIK-type maintenance regions from pKPHS2, the above 15.5-kb IncFIIY-type maintenance region, and the above 5.6-kb IncpA1763-KPC-type backbone region, and it contained a primary Inc-FIIpHN7A8 replicon and two auxiliary IncpA1763-KPC and IncN1 replicons. Each of p721005-KPC, p504051-KPC, and pA3295-KPC acquired a wealth of accessory modules, carrying a range of intact and residue mobile elements (such as insertion sequences, unit transposons, and integrons) and resistance markers (such as blaKPC, tetA, dfrA, and qnr).ConclusionIn each of p721005-KPC, p504051-KPC, and pA3295-KPC, multiple replicons in coordination with maintenance and conjugation regions of various origins would maintain a broad host range and a stable replication at a steady-state plasmid copy number.

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Genomic characterization of novel IncFII-type multidrug resistant plasmids p0716-KPC and p12181-KPC from Klebsiella pneumoniae

Cited by 22 publications

References 37 publications

Molecular Characterization of an IncFIIk Plasmid Co-harboring blaIMP–26 and tet(A) Variant in a Clinical Klebsiella pneumoniae Isolate

Molecular Characterization of an IncFIIk Plasmid Co-harboring blaIMP–26 and tet(A) Variant in a Clinical Klebsiella pneumoniae Isolate

Molecular characterization and epidemiology of carbapenem non-susceptible Enterobacteriaceae isolated from the Eastern region of Heilongjiang Province, China

<p>Comparative analysis of KPC-2-encoding chimera plasmids with multi-replicon IncR:Inc<sub>pA1763-KPC</sub>:IncN1 or IncFII<sub>pHN7A8</sub>:Inc<sub>pA1763-KPC</sub>: IncN1</p>

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