Genomic characterization of members of the Bet v 1 family: genes coding for allergens and pathogenesis-related proteins share intron positions

Hoffmann‐Sommergruber, Karin; Vanek-Krebitz, M; Radauer, Christian; Wen, Jun; Ferreira, Fátima; Scheiner, Otto; Breiteneder, Heimo

doi:10.1016/s0378-1119(97)00246-1

Cited by 105 publications

(66 citation statements)

References 36 publications

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“…4B). Additionally, Hyp-1 showed significant homology with various other classes of allergens, including Pru-a-1, a major cherry allergen (43.1%) (18), Bet.v.1 (41.5%) from hornbeam (19), and a Fagus salvatica allergen (44.3%) (20) (Fig. 4B).…”

Section: Incubation Of Emodin With Cell-free Total Proteins Isolated mentioning

confidence: 99%

“…Proteins belonging to the Bet.v.1 family are the main cause of pollen-related food allergies (21,22), and this protein family shows significant (35-60%) homology to PR (pathogenesis-related)-10 type proteins (23)(24)(25), which are expressed in plant tissues upon microbial infection and chemical treatment (26,27). The deduced amino acid sequence for Hyp-1 comprises several of the signature features of Bet.v.1 class allergens (19). A conserved motif present in Hyp-1 is common throughout all the allergen-coding sequences from the Bet.v.1 family as well as in the PR-10 intracellular PR proteins.…”

Section: Incubation Of Emodin With Cell-free Total Proteins Isolated mentioning

confidence: 99%

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Molecular and Biochemical Characterization of an Enzyme Responsible for the Formation of Hypericin in St. John's Wort (Hypericum perforatum L.)

Bais

Vepachedu

Lawrence

et al. 2003

Journal of Biological Chemistry

118

149

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A major gene termed Hyp-1 encoding for hypericin (HyH) biosynthesis was cloned and characterized from Hypericum perforatum (St. John's wort) cell cultures. H. perforatum leaves are widely used as an herbal remedy in the treatment of mild to moderate depression. Hypericin, a photosensitive and red-colored naphthodianthrone, has been reported as the bioactive compound responsible for reversing the depression symptoms. In this study a novel red-color-based colony screening method for examining a cDNA library (-TriplEX2) derived from H. perforatum cell cultures revealed the gene responsible for hypericin biosynthesis after the administration of emodin, a precursor of hypericin. The selected clones were expressed in Escherichia coli (BM 25.8 line) and were further screened for biosynthesis of emodin to hypericin, which resulted in an 84.6% conversion. The full-length cDNA sequence of Hyp-1 is 782 nucleotides in length with an open reading frame of 477 nucleotides coding for a protein of 159 amino acids, with a 45.1% homology to Bet.v.1 class allergens. Reverse transcriptase-PCR analysis showed high levels of Hyp-1 transcripts in dark-grown cell cultures compared with the levels in light-grown cell cultures and leaves. Southern blot analysis showed the presence of a single Hyp-1 gene in H. perforatum. Furthermore, Hyp-1 was expressed with a His 6 affinity tag linked to its N terminal region using the expression vector pET-28a, and the recombinant Hyp-1 protein was able to convert HyH from emodin under in vitro conditions. HyH product inhibition was observed with emodin analogues, rhein, rhein methyl ester, and DNA3-55-1. Our results demonstrate a direct and complex conversion of emodin to HyH that is solely catalyzed by Hyp-1, a Bet.v.1 class allergen from H. perforatum.

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Section: Incubation Of Emodin With Cell-free Total Proteins Isolated mentioning

confidence: 99%

Section: Incubation Of Emodin With Cell-free Total Proteins Isolated mentioning

confidence: 99%

Molecular and Biochemical Characterization of an Enzyme Responsible for the Formation of Hypericin in St. John's Wort (Hypericum perforatum L.)

Bais

Vepachedu

Lawrence

et al. 2003

Journal of Biological Chemistry

118

149

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“…PR proteins are grouped into 17 families (PR1 to PR17) based on sequence homology, serological relationships and biological activity (Van Loon et al, 1994;Hoffmann-Sommergruber et al, 1997;Van Loon et al, 2006;Christensen et al, 2002). Among these PR families, PR10 proteins are small, acidic intracellular proteins and are encoded by multiple genes; they are a class of intracellular proteins, in contrast to the extracellular nature of most PR proteins.…”

Section: Introductionmentioning

confidence: 99%

Cloning and Characterization of a Pathogenesis-Related Gene (ThPR10) from Tamarix hispida

Zhang¹,

Wang²,

Li³

2010

Acta Biologica Cracoviensia Series Botanica

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A PR10 gene (ThPR10) was cloned from Tamarix hispida and characterized. Real-time RT-PCR was employed to evaluate gene expression levels. ThPR10 was expressed in both leaves and roots of T. hispida under normal growth conditions, and can be highly induced in both leaf and root tissues by abiotic stresses including NaCl, PEG, cold, CdCl 2 , and ABA (abscisic acid) treatments. Our results indicated that ThPR10 is involved in the abiotic stress response, and regulated by an ABA-dependent signaling pathway. Subsequently, ThPR10 was localized at the subcellular level. The gene was fused with the GFP N-terminal driven by CaMV35S promoter and transiently expressed in onion epidermal cells. This strategy localized the ThPR10 protein to the nucleus of onion epidermal cells, suggesting that the pathogenesis-related proteins play a functional role in the cell nucleus.K Ke ey y w wo or rd ds s: : Abiotic stress, gene expression, pathogenesis-related protein, subcellular location, Tamarix hispida. ACTA BIOLOGICA CRACOVIENSIA Series

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“…Over 30 Mal d 1 DNA sequences obtained from cDNA and gDNA of leaves and fruits of various apple cultivars (Vanek-Krebitz et al 1995;Hoffmann-Sommergruber et al 1997;Son et al 1999;Holm et al 2001;Ziadi et al 2001) were published in the database GenBank at the beginning of this study. Their phylogenetic tree indicated four distinct groups with seven members (Puehringer et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Genomic cloning and linkage mapping of the Mal d 1 (PR-10) gene family in apple (Malus domestica)

et al. 2005

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Fresh apples can cause birch pollen-related food allergy in northern and central European populations, primarily because of the presence of Mal d 1, the major apple allergen that is cross-reactive to the homologous and sensitizing allergen Bet v 1 from birch. Apple cultivars differ significantly in their allergenicity. Knowledge of the genetic basis of these differences would direct breeding for hypoallergenic cultivars. The PCR genomic cloning and sequencing were performed on two cultivars, Prima and Fiesta, which resulted in 37 different Mal d 1 gDNA sequences. Based on the mapping of sequence-specific molecular markers, these sequences appeared to represent 18 Mal d 1 genes. Sixteen genes were located in two clusters, one cluster with seven genes on linkage group (LG) 13, and the other cluster with nine genes on the homoeologous LG 16. One gene was mapped on LG 6, and one remained unmapped. According to sequence identity, these 18 genes could be subdivided into four subfamilies. Subfamilies I-III had an intron of different size that was subfamily and genespecific. Subfamily IV consisted of 11 intronless genes. The deduced amino acid sequence identity varied from 65% to 81% among subfamilies, from 82% to 100% among genes within a subfamily, and from 97.5% to 100% among alleles of one gene. This study provides a better understanding of the genetics of Mal d 1 and the basis for further research on the occurrence of allelic diversity among cultivars in relation to allergenicity and their biological functions.

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Genomic characterization of members of the Bet v 1 family: genes coding for allergens and pathogenesis-related proteins share intron positions

Cited by 105 publications

References 36 publications

Molecular and Biochemical Characterization of an Enzyme Responsible for the Formation of Hypericin in St. John's Wort (Hypericum perforatum L.)

Molecular and Biochemical Characterization of an Enzyme Responsible for the Formation of Hypericin in St. John's Wort (Hypericum perforatum L.)

Cloning and Characterization of a Pathogenesis-Related Gene (ThPR10) from Tamarix hispida

Genomic cloning and linkage mapping of the Mal d 1 (PR-10) gene family in apple (Malus domestica)

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