Genomic characterization of human papillomavirus type 13, associated to multifocal epithelial hyperplasia, in a Mayan community

Conde-Ferráez, Laura; Ek-Hernández, Gemaly Elisama; Canché-Pech, José Reyes; Gómez-Carballo, Jesús; Kantún-Moreno, Nuvia; González-Losa, Marı́a del Refugio

doi:10.1016/j.meegid.2020.104595

Cited by 3 publications

(8 citation statements)

References 14 publications

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“…A successful RCA was verified by a rare cutting restriction enzyme, BsmBI (New England, Biolabs) according to described in [1] to linearize the amplified genomes. The digestions were visualized by 0.6% agarose gel electrophoresis in 1X TAE Buffer and ethidium bromide staining.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…Bands were visualized by staining with ethidium bromide on a Gel Doc XR system (Bio-rad) and picture processed with Image lab v.2.0.1 software. To purify the expected PCR products, ExoSAP-IT (USB, Cleveland) was used according to described in [1] .…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…After trimmed and final assembly, we reported a length of 7831 bp for HPV13 genome from Yucatan (referred to as HPV13_YUC). Coding sequences were annotated, and viral proteins traduced [1] . The information was deposited in GenBank with accession number MT068446.…”

Section: Data Descriptionmentioning

confidence: 99%

“…Therefore, genomic research that contributes to knowledge of viral components involved in HPV13 pathogenesis, and molecular mechanisms associated to multifocal epithelial hyperplasia (MEH) disease are urged. In the accompanying paper [1] , we aimed to obtain the complete genome sequence of HPV13 associated to MEH disease, obtained from a Mayan boy living in Yucatan, Mexico. Coding sequences were annotated, and viral proteins traduced and deposited in GenBank with accession number MT068446.…”

mentioning

confidence: 99%

“…We hope that our solutions will help researchers who do not have next-generation sequencing (NGS) platforms. A more comprehensive analysis of this data may be obtained from “Genomic characterization of Human papillomavirus type 13, associated to Multifocal Epithelial Hyperplasia, in a Mayan community” [1] .…”

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confidence: 99%

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Human papillomavirus type 13: Genome amplification and characterization data

et al. 2021

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As for 2020 only two complete genomes of Human papillomavirus type 13 (HPV13) are publicly available in GenBank database. In addition, reports of partial sequences of genetic regions are very limited. Therefore, genomic research that contributes to knowledge of viral components involved in HPV13 pathogenesis, and molecular mechanisms associated to multifocal epithelial hyperplasia (MEH) disease are urged. In the accompanying paper [1] , we aimed to obtain the complete genome sequence of HPV13 associated to MEH disease, obtained from a Mayan boy living in Yucatan, Mexico. Coding sequences were annotated, and viral proteins traduced and deposited in GenBank with accession number MT068446. In this data report, we present the oligonucleotide list used to amplify the complete genome, a graphical abstract of process employed for the amplification of circular HPV13 genome, a representative figure of PCR products obtained for sequencing and multiple sequence alignments with the translated coding sequences of the existing genomes: X62843 is the first HPV13 genome reported [2] ; it was generated from a clone obtained from a Turkish patient; DQ344807 was originally obtained from a patient in the Amazonian region [3] . The multiple sequence alignments show the main viral proteins (predicted). This provides relevant information for future molecular analysis and epidemiological studies because HPV13 is an understudied genotype associated to a neglected disease that appears more commonly in children. Additionally, the description of the methods can help in future sequencing of HPV genomes. We hope that our solutions will help researchers who do not have next-generation sequencing (NGS) platforms. A more comprehensive analysis of this data may be obtained from “Genomic characterization of Human papillomavirus type 13, associated to Multifocal Epithelial Hyperplasia, in a Mayan community” [1] .

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Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Section: Data Descriptionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Human papillomavirus type 13: Genome amplification and characterization data

et al. 2021

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HPV Infections—Classification, Pathogenesis, and Potential New Therapies

Mlynarczyk-Bonikowska,

Rudnicka

2024

IJMS

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To date, more than 400 types of human papillomavirus (HPV) have been identified. Despite the creation of effective prophylactic vaccines against the most common genital HPVs, the viruses remain among the most prevalent pathogens found in humans. According to WHO data, they are the cause of 5% of all cancers. Even more frequent are persistent and recurrent benign lesions such as genital and common warts. HPVs are resistant to many disinfectants and relatively unsusceptible to external conditions. There is still no drug available to inhibit viral replication, and treatment is based on removing lesions or stimulating the host immune system. This paper presents the systematics of HPV and the differences in HPV structure between different genetic types, lineages, and sublineages, based on the literature and GenBank data. We also present the pathogenesis of diseases caused by HPV, with a special focus on the role played by E6, E7, and other viral proteins in the development of benign and cancerous lesions. We discuss further prospects for the treatment of HPV infections, including, among others, substances that block the entry of HPV into cells, inhibitors of viral early proteins, and some substances of plant origin that inhibit viral replication, as well as new possibilities for therapeutic vaccines.

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Focal Epithelial Hyperplasia

Bendtsen

Jakobsen

Carlander

et al. 2021

Viruses

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Focal epithelial hyperplasia (FEH) or Heck’s disease is a rare, benign, oral condition that is associated with infection by human papillomavirus type 13, 32 or both. The whiteish to mucosal-colored, soft, papular or nodular elevated lesions in the oral cavity are normally asymptomatic but can grow to a size or at a location where treatment is needed. The diagnosis is often based on clinical presentation and histopathology, and the HPV genotype can be determined using PCR utilizing specific primers or DNA sequencing. While FEH was reported to often affect several members of the same family and exist primarily among indigenous populations around the world, the number of reported cases within the European region is increasing. This contemporary review summarizes the main findings in relation to HPV genotypes, impact of superinfection exclusion and vaccination, transmission, diagnosis, geographical and ethnical distribution, comorbidities and treatment of FEH with an emphasis on including the most recent case reports within the field. Furthermore, we describe for the first time a FEH lesion infected with the low-risk HPV90.

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Genomic characterization of human papillomavirus type 13, associated to multifocal epithelial hyperplasia, in a Mayan community

Cited by 3 publications

References 14 publications

Human papillomavirus type 13: Genome amplification and characterization data

Human papillomavirus type 13: Genome amplification and characterization data

HPV Infections—Classification, Pathogenesis, and Potential New Therapies

Focal Epithelial Hyperplasia

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