Genomic characterization of Arcanobacterium pyogenes isolates recovered from the uterus of dairy cows with normal puerperium or clinical metritis

Silva, E.; Gaivão, Maria; Leitão, Sara; Jost, B. Helen; Carneiro, C.; Vilela, C. L.; Lopes-da-Costa, Luís; Mateus, L.

doi:10.1016/j.vetmic.2008.04.033

Cited by 72 publications

(91 citation statements)

References 26 publications

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“…16 Each PCR was carried out using a commercial PCR kit. c Each 25-µl reaction contained 1× PCR buffer, 200 µM of each deoxyribonucleotide triphosphate, 0.5 µM of each primer, 2.5 U of DNA polymerase, and 5 µl of template DNA.…”

Section: Research-article2014mentioning

confidence: 99%

Investigation of polymerase chain reaction assays to improve detection of bacterial involvement in bovine respiratory disease

Bell¹,

Blackburn²,

Elliott³

et al. 2014

J VET Diagn Invest

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Bovine respiratory disease (BRD) causes severe economic losses to the cattle farming industry worldwide. The major bacterial organisms contributing to the BRD complex are Mannheimia haemolytica, Histophilus somni, Mycoplasma bovis, Pasteurella multocida, and Trueperella pyogenes. The postmortem detection of these organisms in pneumonic lung tissue is generally conducted using standard culture-based techniques where the presence of therapeutic antibiotics in the tissue can inhibit bacterial isolation. In the current study, conventional and real-time polymerase chain reaction (PCR) assays were used to assess the prevalence of these 5 organisms in grossly pneumonic lung samples from 150 animals submitted for postmortem examination, and the results were compared with those obtained using culture techniques. Mannheimia haemolytica was detected in 51 cases (34%) by PCR and in 33 cases (22%) by culture, H. somni was detected in 35 cases (23.3%) by PCR and in 6 cases (4%) by culture, Myc. bovis was detected in 53 cases (35.3%) by PCR and in 29 cases (19.3%) by culture, P. multocida was detected in 50 cases (33.3%) by PCR and in 31 cases (20.7%) by culture, and T. pyogenes was detected in 42 cases (28%) by PCR and in 31 cases (20.7%) by culture, with all differences being statistically significant. The PCR assays indicated positive results for 111 cases (74%) whereas 82 cases (54.6%) were culture positive. The PCR assays have demonstrated a significantly higher rate of detection of all 5 organisms in cases of pneumonia in cattle in Northern Ireland than was detected by current standard procedures.

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Section: Research-article2014mentioning

confidence: 99%

Investigation of polymerase chain reaction assays to improve detection of bacterial involvement in bovine respiratory disease

Bell¹,

Blackburn²,

Elliott³

et al. 2014

J VET Diagn Invest

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“…Endometrial epithelial cells, as the first line of defense, can initiate an immune reaction by increased synthesis of different cytokines [interleukin 1A ( IL1A ), IL6, IL8 , and CXC ligand 3 ( CXCL3 )] and prostaglandin endoperoxide synthase 2 ( PTGS2 ; Amos et al, 2014; Turner et al, 2014; Gärtner et al, 2016), leading to an influx of immune cells to the site of infection (Takeuchi and Akira, 2010). Although T. pyogenes is associated with severe endometritis, it has also been isolated from cows without signs of uterine disease at puerperium (Silva et al, 2008; Santos et al, 2010; Wagener et al, 2015). …”

Section: Introductionmentioning

confidence: 99%

Bovine Endometrial Epithelial Cells Scale Their Pro-inflammatory Response In vitro to Pathogenic Trueperella pyogenes Isolated from the Bovine Uterus in a Strain-Specific Manner

Ibrahim

Peter

Wagener

et al. 2017

Front. Cell. Infect. Microbiol.

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Among different bacteria colonizing the bovine uterus, Trueperella pyogenes is found to be associated with clinical endometritis (CE). The ability of cows to defend against T. pyogenes infections depends on the virulence of invading bacteria and on the host's innate immunity. Therefore, to gain insights into bacterial factors contributing to the interplay of this host pathogen, two strains of T. pyogenes were included in this study: one strain (TP2) was isolated from the uterus of a postpartum dairy cow developing CE and a second strain (TP5) was isolated from a uterus of a healthy cow. The two strains were compared in terms of their metabolic fingerprints, growth rate, virulence gene transcription, and effect on bovine endometrial epithelial cells in vitro. In addition, the effect of the presence of peripheral blood mononuclear cells (PBMCs) on the response of endometrial epithelial cells was evaluated. TP2, the strain isolated from the diseased cow, showed a higher growth rate, expressed more virulence factors (cbpA, nanH, fimE, and fimG), and elicited a higher mRNA expression of pro-inflammatory factors (PTGS2, CXCL3, and IL8) in bovine endometrial epithelial cells compared with TP5, the strain isolated from the healthy cow. The presence of PBMCs amplified the mRNA expression of pro-inflammatory factors (PTGS2, CXCL3, IL1A, IL6, and IL8) in bovine endometrial epithelial cells co-cultured with live TP2 compared with untreated cells, especially as early as after 4 h. In conclusion, particular strain characteristics of T. pyogenes were found to be important for the development of CE. Furthermore, immune cells attracted to the site of infection might also play an important role in up-regulation of the pro-inflammatory response in the bovine uterus and thus significantly contribute to the host-pathogen interaction.

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“…Arcanobacterium pyogenes is considered an important opportunistic pathogen of the upper respiratory tract and in puerperal uterine infections of cattle, sheep, swine, birds, humans and many other species (Nattermann & Horsch, 1977;Queen et al, 1994;Narayanan et al, 1998;Silva et al, 2008). It was recently reclassified from the genus Actinomyces on the basis of the rRNA gene sequence (Pascual Ramos et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

“…Dyspepsia, pneumonia, metritis, urinary stones and abscesses are common diseases of forest musk deer here, and abscesses in particular have cause hundreds of deaths. Antibiotics have been used here for over 15 years, but due to the previous negligent management where drug abuse was prevalent and the dose was always on the high side, resistance has developed, meaning that many of the drugs have become useless against some of the pathogenic infections.Arcanobacterium pyogenes is considered an important opportunistic pathogen of the upper respiratory tract and in puerperal uterine infections of cattle, sheep, swine, birds, humans and many other species (Nattermann & Horsch, 1977;Queen et al, 1994;Narayanan et al, 1998;Silva et al, 2008). It was recently reclassified from the genus Actinomyces on the basis of the rRNA gene sequence (Pascual Ramos et al, 1997).…”

mentioning

confidence: 99%

Detection and characterization of antibiotic-resistance genes in Arcanobacterium pyogenes strains from abscesses of forest musk deer

Zhao

Liu

Zhang

et al. 2011

Journal of Medical Microbiology

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Arcanobacterium pyogenes is commonly isolated from ruminant animals as an opportunistic pathogen that co-infects with other bacteria, normally causing surface or internal abscesses. Twenty-eight strains of A. pyogenes isolated from forest musk deer suppurative samples were identified by their 16S rRNA gene sequences, and confirmed by amplification of the pyolysinencoding gene (plo) in all isolates. The MICs of 14 commonly used antibiotics were determined by an agar dilution method. Class 1 and 2 intI genes were amplified to determine whether integrons were present in the A. pyogenes genome. Class 1 gene cassettes were detected by specific primers and analysed by sequencing. All of the strains were susceptible to most fluoroquinolone antibiotics; however, high resistance rates were observed for b-lactams and trimethoprim. A total of 18 of the isolates (64.3 %) were positive for the class 1 intI gene, and 16 (57.1 %) contained class 1 gene cassettes with the aacC, aadA1, aadA2, blaP1 and dfr2a genes. Most were present in the multi-resistant isolates, indicating a general concordance between the presence of gene cassettes and antibiotic resistance, and that the integrons have played an important role in the dissemination of antimicrobial resistance in this species. INTRODUCTIONForest musk deer (Moschus berezovskii) are solitary ruminants encountered mainly in South-West China. The musk secreted by the deer is a traditional and precious Chinese medicine, and is also used in the manufacture of perfumes. As a result of the decreasing population size, all forest musk deer species were categorized as first-class key species of wildlife protected by Chinese legislation in 2002 (Chen et al., 2007;Guha et al., 2007;Guan et al., 2009). Artificial breeding of forest musk deer started in the 1950s, and diseases were the most significant restriction factor preventing a population increase (Lu et al., 2009). In Miyaluo Farm (Sichuan Province, China), every eight to ten forest musk deer are fed in one fold of about 200 m 2 due to the timid and leaping character of these animals, which means that they easily hurt themselves. Dyspepsia, pneumonia, metritis, urinary stones and abscesses are common diseases of forest musk deer here, and abscesses in particular have cause hundreds of deaths. Antibiotics have been used here for over 15 years, but due to the previous negligent management where drug abuse was prevalent and the dose was always on the high side, resistance has developed, meaning that many of the drugs have become useless against some of the pathogenic infections.Arcanobacterium pyogenes is considered an important opportunistic pathogen of the upper respiratory tract and in puerperal uterine infections of cattle, sheep, swine, birds, humans and many other species (Nattermann & Horsch, 1977;Queen et al., 1994;Narayanan et al., 1998;Silva et al., 2008). It was recently reclassified from the genus Actinomyces on the basis of the rRNA gene sequence (Pascual Ramos et al., 1997). The organisms enter the blood stream and interact...

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Genomic characterization of Arcanobacterium pyogenes isolates recovered from the uterus of dairy cows with normal puerperium or clinical metritis

Cited by 72 publications

References 26 publications

Investigation of polymerase chain reaction assays to improve detection of bacterial involvement in bovine respiratory disease

Investigation of polymerase chain reaction assays to improve detection of bacterial involvement in bovine respiratory disease

Bovine Endometrial Epithelial Cells Scale Their Pro-inflammatory Response In vitro to Pathogenic Trueperella pyogenes Isolated from the Bovine Uterus in a Strain-Specific Manner

Detection and characterization of antibiotic-resistance genes in Arcanobacterium pyogenes strains from abscesses of forest musk deer

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