Genomic characterization and phylogenetic analysis of Zika virus circulating in the Americas

Ye, Qing; Liu, Zhong-Yu; Han, Jianfeng; Jiang, Tao; Li, Xiaofeng; Qin, Cheng‐Feng

doi:10.1016/j.meegid.2016.05.004

Cited by 102 publications

(105 citation statements)

References 58 publications

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“…Furthermore, the sequences of clinical samples isolated from the American outbreak are highly conserved, and are therefore now recognized as a new clade of the Asian lineage (Enfissi et al, 2016; Wang et al, 2016; Ye et al, 2016). Using genomic sequence alignments, a comparison between historic Asian lineage isolates, currently circulating American clade isolates and original African lineage isolates demonstrates how divergent the American clade has become from the African lineage ( Table 3 ).…”

Section: Discussionmentioning

confidence: 99%

“…This may be due to antigenicity difference between African and Asian lineage viruses but most likely is due to the failure of the virus to establish a productive infection. Numerous studies have now been published on comparative genomics and epidemiological analysis aimed at identifying critical adaptive mutation(s) or processes such as codon utilization or glycosylation that may contribute to the pathogenicity and/or fitness in transmission of the emergent American clade (Gupta et al, 2016; van Hemert and Berkhout, 2016; Ye et al, 2016). …”

Section: Discussionmentioning

confidence: 99%

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Development of a Zika Virus Infection Model in Cynomolgus Macaques

et al. 2016

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Limited availability of Indian rhesus macaques (IRM) is a bottleneck to study Zika virus (ZIKV) pathogenesis and evaluation of appropriate control measures in non-human primates. To address these issues, we report here the Mauritian cynomolgus macaque (MCM) model for ZIKV infection. In brief, six MCMs (seronegative for Dengue and ZIKV) were subdivided into three cohorts with a male and female each and challenged with different doses of Asian [PRVABC59 (Puerto Rico) or FSS13025 (Cambodia)] or African (IBH30656) lineage ZIKV isolates. Clinical signs were monitored; and biological fluids (serum, saliva, and urine) and tissues (testes and brain) were assessed for viral load by quantitative reverse transcription polymerase chain reaction and neutralizing antibodies (Nab) by 50% Plaque Reduction Neutralization Test (PRNT50) at various times post-infection (p.i). PRVABC59 induced viremia detectable up to day 10, with peak viral load at 2–3 days p.i. An intermittent viremia spike was observed on day 30 with titers reaching 2.5 × 103 genomes/mL. Moderate viral load was observed in testes, urine and saliva. In contrast, FSS13025 induced viremia lasting only up to 6 days and detectable viral loads in testes but not in urine and saliva. Recurrent viremia was detected but at lower titers compare to PRVABC59. Challenge with either PRVABC59 or FSS13025 resulted in 100% seroconversion; with mean PRNT50 titers ranging from 597 to 5179. IBH30656 failed to establish infection in MCM suggesting that MCM are susceptible to infection with ZIKV isolates of the Asian lineage but not from Africa. Due to the similarity of biphasic viremia and Nab responses between MCM and IRM models, MCM could be a suitable alternative for evaluation of ZIKV vaccine and therapeutic candidates.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Development of a Zika Virus Infection Model in Cynomolgus Macaques

et al. 2016

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“…The stem-loop structure of 3’UTR of ZIKV genome is presented as previously reported 33,34 . The nucleotide sequence of the shaded stem-loop is shown.…”

Section: Extended Datamentioning

confidence: 95%

A live-attenuated Zika virus vaccine candidate induces sterilizing immunity in mouse models

Shan

Muruato

Nunes

et al. 2017

Nat Med

234

243

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Zika virus (ZIKV) infection of pregnant patients could cause a wide range of congenital abnormalities (including microcephaly) now collectively known as congenital ZIKV syndrome1. A vaccine to prevent or significantly attenuate viremia in pregnant women and travelers to epidemic/endemic regions is needed to avert congenital ZIKV syndrome, and could also be useful to suppress epidemic transmission. Here we report a live-attenuated vaccine candidate that contains a 10-nucleotide deletion in the 3’ untranslated region of ZIKV genome (10-del ZIKV). The 10-del ZIKV is highly attenuated, immunogenic, and protective in the A129 mouse model. Critically, a single dose of 10-del ZIKV induced sterilizing immunity with a high level of neutralizing antibodies and completely prevented viremia after challenge. The immunized mice also developed a robust T cell response. Intracranial inoculation of one-day-old CD1 mice with 1×104 IFU of 10-del ZIKV caused no detectable disease, whereas infections with 10 IFU of wild-type ZIKV were lethal. Mechanistically, the 10-del ZIKV attenuated its virulence through decreased viral RNA synthesis and increased sensitivity to type-I interferon inhibition. The attenuated 10-del ZIKV was incompetent in infecting mosquitoes after oral feeding of spiked blood meals, representing an additional safety feature for use in non-endemic regions. Collectively, the safety and efficacy results warrant further development of this promising live-attenuated ZIKV vaccine candidate.

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“…Its genome of 10.8 kb encodes a single polyprotein which is processed 29 into three structural proteins (C, PrM and E), and seven nonstructural proteins (NS1, NS2A, 30 NS2B, NS3, NS4A, NS4B and NS5) by viral and host proteases [1]. Phylogenetic studies 31…”

Section: Introduction 27mentioning

confidence: 99%

“…antigenicity [1]. In almost all cases, the well-characterized live attenuated 17-D strain used to 53 prevent yellow fever virus (YFV) infections has been used as the genetic backbone.…”

Section: Introduction 27mentioning

confidence: 99%