Genomic characterization and molecular investigation of VP7 epitopes of uncommon G10P[8] group A rotavirus strains detected in Italy in 2009

Ianiro, Giovanni; Delogu, Roberto; Fiore, Lucia; Ruggeri, Franco Maria

doi:10.1099/vir.0.000123

Cited by 6 publications

(5 citation statements)

References 51 publications

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“…In this study, only one child of 55 enrolled in this study had received rotavirus immunization. The rotavirus genotype identified in the sample collected from this patients was genotyped as G10P[8], a typical bovine rotavirus genotype considered uncommon among humans, and previously detected in Italy only in 2009 . The detection of this rare bovine‐derived RVA genotype in a vaccinated child might be partly related to a lower heterotypic response induced by the licensed anti‐RV vaccines available, which do not include the G10 protein specificity.…”

Section: Discussionmentioning

confidence: 99%

Hospital‐acquired rotavirus and norovirus acute gastroenteritis in a pediatric unit, in 2014‐2015

Valentini

Ianiro

Bartolo

et al. 2017

Journal of Medical Virology

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The occurrence of hospital-acquired acute gastroenteritis (AGE) is a major concern for public health. RotavirusA (RVA) and norovirus (NoV) are common causes of viral AGE in the pediatric population, and their role in nosocomial infections has been proven, remaining poorly investigated. To investigate RVA and NoV in hospital-acquired AGE, 55 stool samples from children with nosocomial AGE were collected between May 2014 and May 2015. To evaluate virus spreading routes, 51 environmental swabs were collected from staff and patients' rooms. Stools were tested for both RVA and NoV RNA by reverse-transcription-PCR. Nucleotide sequencing and phylogenetic analysis were performed to characterize the viruses. Forty-seven of 55 cases analyzed resulted positive for RVA. The predominant genotype was G4P[8] (18/55) followed by G1P[8] (14/55). Mixed RVA infections were also detected (7/55). Twenty-two samples were positive for NoV, and GII.4 was revealed to be the predominant genotype. Seventeen samples were positive for both RVA and NoV. This study aimed to evaluate the burden of norovirus and rotavirus nosocomial AGE, contributing to identify the environment source of infections and to activate effective strategies for intervention. The reduction in nosocomial AGE cases is an important aspect, considered the worsened disease course in transplant, cancer, and intensive care unit inpatients.

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Section: Discussionmentioning

confidence: 99%

Hospital‐acquired rotavirus and norovirus acute gastroenteritis in a pediatric unit, in 2014‐2015

Valentini

Ianiro

Bartolo

et al. 2017

Journal of Medical Virology

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“…Of these RV strains, Wa‐like genotype constellation is usually associated with G1, G3, G4, G9, and P[8] RVA strains, while DS‐1 like constellation with G2 and P[4]. However, variable combinations of G/P genotypes of human RV strains have been reported earlier …”

Section: Introductionmentioning

confidence: 99%

“…However, variable combinations of G/P genotypes of human RV strains have been reported earlier. 8,[13][14][15] Ever since 1995, rapid spread of G9 RVA has been reported worldwide making them now the major human RVA genotype. 13,[16][17][18][19] Variable prevalence rates of G9 RVA strains have been also reported between 27% and 62% among geographical areas like Cuba, France, Mexico, and USA where G9 RVs strains have emerged.…”

Section: Introductionmentioning

confidence: 99%

Full genome analysis of rotavirus G9P[8] strains identified in acute gastroenteritis cases reveals genetic diversity: Pune, western India

Tatte

Chaphekar

Gopalkrishna

2017

Journal of Medical Virology

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Group A rotaviruses (RVA) are the major enteric etiological agents of severe acute gastroenteritis among children globally. As G9 RVA now represents as one of the major human RVA genotypes, studies on full genome of this particular genotype are being carried out worldwide. So far, no such studies on G9P[8] RVAs have been reported from Pune, western part of India. Keeping in view of this, the study was undertaken to understand the degree of genetic diversity of the commonly circulating G9P[8] RVA strains. Rotavirus surveillance studies carried out earlier during the years 2009-2011 showed increase in the prevalence of G9P[8] RVAs. Representative G9P[8] RVA strains from the years 2009, 2010, and 2011 were selected for the study. In general, all the G9 RVA strains showed clustering in the globally circulating sublineage of the VP7 gene and showed nucleotide/amino acid identities of 96.8-99.7%/96.9-99.8% with global G9 RV strains. Full genome analysis, of all three RVAs in this study indicated Wa-like genotype constellation G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1. Within the strains nucleotide/amino acid divergence of 0.1-3.4%/0.0-4.1% was noted in all the RVA structural and non-structural genes. In conclusion, the present study highlights intra-genotypic variations throughout the RVA genome. The study further emphasizes the need for surveillance and analysis of the whole genomic constellation of the commonly circulating RVA strains of other regions in the country for understanding to a greater degree of the impact of rotavirus vaccination recently introduced in India.

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“…They are characterized by substantial genetic diversity, as evidenced by the presence of multiple serotypes (Bernstein, 2009). The VP 7 and VP4 encoding gene segments are the basis of a binary classification system (Ianiro, et al, 2015;Desselberger et al, 2009). So, viruses are referred to as G-types and P-Types (Greenberg & Estes, 2009).…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Genetic Characterization of Rotavirus Infections Among Children Under Five Years in Mutaho Health District, Gitega Province and Bujumbura Municipality, Burundi

2019

JBAH

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Rotavirus is the leading cause of severe diarrhea in children under five years worldwide. It is ranked as a priority for vaccine. In Burundi, vaccine against rotavirus was implemented in 2013. The impact of recent rotavirus vaccination on morbidities in Burundi is not well established. Moreover, no study has been carried out to document the genetic diversity of rotavirus strains circulating in Burundi. This cross-sectional health facilitybased study aimed at determining the prevalence and molecular characteristics of rotavirus infections among children under five years of age in Mutaho Health District and the Municipality of Bujumbura, in Burundi. Stool specimens were collected from children presenting with acute diarrhea. These specimens were tested for rotavirus antigen using Diagnostar ® rapid test kit. Positive stool samples were confirmed at the Kenya Medical Research Institute (KEMRI) by ELISA. Positive confirmed samples underwent RT-PCR, G and P genotyping by multiplex semi-nested PCR using a cocktail of type specific primers or by sequencing. A total of 646 participants were enrolled in this study. The overall prevalence of rotavirus was 6.2% (40/646) with 4.0% (16/400) in Mutaho health district and 9.7% (24/246) in the Municipality of Bujumbura. Rotavirus detection rate tended to increase as the level of precipitation went down, showing a significant negative association between the two variables. (OR = 15.2; P = 0.0001). In addition, rotavirus detection rate was higher in Bujumbura Municipality than in Mutaho health district (OR = 2.6; P = 0.005). Two G genotypes were identified, G1 the predominating G genotype accounted for 53.8% (14/26) followed by G12 (46.2%, 12/26). The prevalence of the genotype G1 of Group A rotavirus was significantly higher in Bujumbura Municipality than in Mutaho health district while G12 predominated in Mutaho health district (OR = 7.33; P = 0.026). Rotavirus strains from pigs might have contributed to the high prevalence of human G12 rotavirus in that area. Three different P types were identified P[8] the most common, followed by P[6] and P[4]. The most common G/P combination genotype was G1P[8] which accounted for 45.5% of all rotavirus genotypes identified, followed by G12 P [8] (41.0%), G1P [6] (4.5%), G12 P [6] (4.5%) and G12 P [4] (4.5%). The emergence of G12 rotavirus strains which share neither G nor P genotypes with currently used rotavirus vaccines raises public health concerns as they have the potential to challenge their efficacy. Therefore, we recommend to initiate and maintain a continuous rotavirus strain surveillance in Burundi so as to monitor trends in the occurrence of these prevailing and potentially emerging new strains.

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Genomic characterization and molecular investigation of VP7 epitopes of uncommon G10P[8] group A rotavirus strains detected in Italy in 2009

Cited by 6 publications

References 51 publications

Hospital‐acquired rotavirus and norovirus acute gastroenteritis in a pediatric unit, in 2014‐2015

Hospital‐acquired rotavirus and norovirus acute gastroenteritis in a pediatric unit, in 2014‐2015

Full genome analysis of rotavirus G9P[8] strains identified in acute gastroenteritis cases reveals genetic diversity: Pune, western India

Prevalence and Genetic Characterization of Rotavirus Infections Among Children Under Five Years in Mutaho Health District, Gitega Province and Bujumbura Municipality, Burundi

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