Genomic characteristics and pathogenicity of a new recombinant strain of porcine reproductive and respiratory syndrome virus

Li, Yang; Xu, Gaoxiao; Du, Xingqian; Xu, Liang; Ma, Zhien; Li, Zhiwei; Feng, Yingtong; Jiao, Dian; Guo, Wen‐Ping; Xiao, Shuqi

doi:10.1007/s00705-020-04917-8

Cited by 11 publications

(5 citation statements)

References 49 publications

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“…Compared with other PRRSV lineages in China, the NADC30-like strain has a higher potential for recombination and pathogenic diversity. Numerous recent studies ( Zhao et al, 2015 ; Zhang et al, 2016 ; Liu et al, 2018 ; Sui et al, 2018 ; Han et al, 2019 ; Zhang et al, 2019 ; Li et al, 2021 ; Liu et al, 2022 ; Ma et al, 2022 ; Wu et al, 2022 ; Chang et al, 2023 ) have indicated that the virulence of NADC30-like PRRSV strains is related to the recombination regions and segments derived from the parental strains. Currently, all NADC30-like PRRSV strains induce typical clinical symptoms and pathological changes in piglets following infection, with their pathogenicity tending toward intermediacy between the parental strains ( Yu et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Isolation, identification, recombination analysis and pathogenicity experiment of a PRRSV recombinant strain in Sichuan Province, China

Tu,

Li,

Zhang

et al. 2024

Front. Microbiol.

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Since 2013, the porcine reproductive and respiratory syndrome virus type 2 (PRRSV-2), lineage 1.8 (NADC30-like PRRSV) has emerged and become widely prevalent in China. The NADC30-like PRRSV poses significant challenges for disease control, primarily because of its propensity for frequent mutations and recombinations. We successfully isolated and identified a NADC30-like strain, designated SCCD22, in Chengdu, Sichuan Province, China. We meticulously examined the genetic recombination properties and evaluated its pathogenicity in 28-day-old piglets. SCCD22 showed 93.02% nucleotide homology with the NADC30 PRRSV strain, and its non-structural protein 2 coding region showed the same 131 amino acid deletion pattern as that seen in NADC30. Furthermore, we identified two recombination events in SCCD22: one in the NSP2 region (1,028–3,290 nt), where it was highly similar to the JXA1-like strain GZ106; and another in the NSP10 ~ 12 region (9,985–12,279 nt), closely resembling the NADC30-like strain CY2-1604. Piglets infected with SCCD22 exhibited clinical symptoms such as elevated body temperature, prolonged fever, reduced appetite, and roughened fur. Postmortem examinations underscored the typical lung pathology associated with PRRSV, indicating that the lungs were the primary affected organs. Furthermore, extended viral shedding accompanied by progressive viremia was observed in the serum and nasal excretions of infected piglets. In summary, this study reports a domestic PRRSV recombination strain in the Sichuan Province that can provide critical insights into preventing and controlling PRRSV in this region.

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Section: Introductionmentioning

confidence: 99%

Isolation, identification, recombination analysis and pathogenicity experiment of a PRRSV recombinant strain in Sichuan Province, China

Tu,

Li,

Zhang

et al. 2024

Front. Microbiol.

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“…The results indicated that the HuN-XT-B strain was recombinant from NADC30-like strain and JXA1 (or MLV)-like strain. Many researchers have reported the occurrences of recombinant events between field strains and HP-PRRSV (or MLV like) strains in China [ 3 , 31 ], suggesting safer vaccines against PRRS should be developed to replace the MLV vaccines which have been widely used in the Chinese pig industry.…”

Section: Discussionmentioning

confidence: 99%

Epidemiology and Genetic Characteristics of Porcine Reproductive and Respiratory Syndrome Virus in the Hunan and Hebei Provinces of China

Zhai

Yu²,

Zhang

et al. 2023

Veterinary Sciences

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Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant threat to the pig industry in China. However, the epidemiological characteristics of PRRSV after the outbreak of African swine fever in China were not thoroughly investigated. In the present study, the serological and epidemiological investigations of PRRSV in pigs from the Hunan and Hebei provinces of China were assessed. The results showed that 73.12% (95% CI 71.74–74.49) of pigs were positive for PRRSV-special antibody by enzyme-linked immunosorbent assay. Out of 5799 samples, 482 (8.31%, 95% CI 7.60–9.02) samples were positive for PRRSV nucleic acids. The positive rates of PRRSV in healthy pigs from farms and slaughterhouses were 2.27% (47/2072) and 7.70% (217/2818), which were lower than that in diseased pigs (23.98%, 218/909). Furthermore, the full-length OFR5 gene sequences of 43 PRRSV strains were sequenced and analysed. Phylogenetic analysis revealed that 43 isolates were classified into three lineages, namely lineage 1 (n = 24), lineage 8 (n = 15), and lineage 3 (n = 4). Lineage 1 could be further divided into sublineage 1.5 (n = 2) and sublineage 1.8 (n = 22), and lineage 8 was classified into sublineage 8.1 (n = 3) and sublineage 8.7 (n = 12). Collectively, our findings revealed the severe prevalence of PRRSV in the Hunan and Hebei provinces, where sublineage 1.8 and sublineage 8.7 predominated. The present study provides the update information of the epidemiological and genetic characteristics of PRRSV in the investigated regions, which will be beneficial for PRRS control.

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“…PRRSV is an arteritis virus with a capsule ( Karl-Klaus, Nico, et al, 1993 ). It is one of the most serious diseases faced by the global pig industry and causes huge losses to the pig industry every year ( Li et al, 2021 ; Wang, Yu, Cai, Zhou, & Zimmerman, 2020 ).…”

Section: Ade Of Representative Virusmentioning

confidence: 99%

Antibody dependent enhancement: Unavoidable problems in vaccine development

et al. 2021

Advances in Immunology

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Genomic characteristics and pathogenicity of a new recombinant strain of porcine reproductive and respiratory syndrome virus

Cited by 11 publications

References 49 publications

Isolation, identification, recombination analysis and pathogenicity experiment of a PRRSV recombinant strain in Sichuan Province, China

Isolation, identification, recombination analysis and pathogenicity experiment of a PRRSV recombinant strain in Sichuan Province, China

Epidemiology and Genetic Characteristics of Porcine Reproductive and Respiratory Syndrome Virus in the Hunan and Hebei Provinces of China

Antibody dependent enhancement: Unavoidable problems in vaccine development

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