Genomic Approach to the Assessment of Adverse Effects of Particulate Matters on Skin Cancer and Other Disorders and Underlying Molecular Mechanisms

Kee, Nam Gook; Kim, Hyun Soo; Choi, Hyunjung; Kim, Hyoung-June; Seo, Young Rok

doi:10.15430/jcp.2021.26.3.153

Cited by 4 publications

(2 citation statements)

References 77 publications

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“…PM can cause skin diseases, such as atopic dermatitis, acne, skin aging, and cancer ( Kee et al, 2021 ; Kim, Cho & Park, 2016 ; Song et al, 2011 ; Vierkotter et al, 2010 ). PM can penetrate the epidermis and intercellular space through the stratum spinosum with existing hair skin.…”

Section: Discussionmentioning

confidence: 99%

Particulate matter 10 induces oxidative stress and apoptosis in rhesus macaques skin fibroblast

Lee,

Kwon,

et al. 2023

PeerJ

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Background Particulate matter (PM) is a major air pollutant that affects human health worldwide. PM can pass through the skin barrier, thus causing skin diseases such as heat rash, allergic reaction, infection, or inflammation. However, only a few studies have been conducted on the cytotoxic effects of PM exposure on large-scale animals. Therefore, herein, we investigated whether and how PM affects rhesus macaque skin fibroblasts. Methods Rhesus macaque skin fibroblasts were treated with various concentrations of PM10 (1, 5, 10, 50, and 100 μg/mL) and incubated for 24, 48, and 72 h. Then, cell viability assay, TUNEL assay, and qRT-PCR were performed on the treated cells. Further, the reactive oxygen species, glutathione, and cathepsin B levels were determined. The MTT assay revealed that PM10 (>50 μg/mL) proportionately reduced the cell proliferation rate. Results PM10 treatment increased TUNEL-positive cell numbers, following the pro-apoptosis-associated genes (CASP3 and BAX) and tumor suppressor gene TP53 were significantly upregulated. PM10 treatment induced reactive oxidative stress. Cathepsin B intensity was increased, whereas GSH intensity was decreased. The mRNA expression levels of antioxidant enzyme-related genes (CAT, GPX1 and GPX3) were significantly upregulated. Furthermore, PM10 reduced the mitochondrial membrane potential. The mRNA expression of mitochondrial complex genes, such as NDUFA1, NDUFA2, NDUFAC2, NDUFS4, and ATP5H were also significantly upregulated. In conclusion, these results showed that PM10 triggers apoptosis and mitochondrial damage, thus inducing ROS accumulation. These findings provide potential information on the cytotoxic effects of PM10 treatment and help to understand the mechanism of air pollution-induced skin diseases.

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Section: Discussionmentioning

confidence: 99%

Particulate matter 10 induces oxidative stress and apoptosis in rhesus macaques skin fibroblast

Lee,

Kwon,

et al. 2023

PeerJ

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“…The wide variety of resources available for analyzing the genome, together with the required bioinformatic tools have provided unprecedented insights into the structure and function of the genome, transcriptome, proteome, and metabolome [9,10]. Diverse omic analytical methods have been adapted to environmental research, but some limitations and challenges remain that need to be considered to fully reveal the potential and acceptance of toxicogenomic data by industry and regulatory agencies [5,11].…”

Section: Introductionmentioning

confidence: 99%

Perspectives on the Use of Toxicogenomics to Assess Environmental Risk

Portugal¹,

Mansilla²,

Piña³

2022

Front. Biosci. (Landmark Ed)

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Environmental toxicogenomics aims to collect, analyze and interpret data on changes in gene expression and protein activity resulting from exposure to toxic substances using high-performance omics technologies. Molecular profiling methods such as genomics, transcriptomics, proteomics, metabolomics, and bioinformatics techniques, permit the simultaneous analysis of a multitude of gene variants in an organism exposed to toxic agents to search for genes prone to damage, detect patterns and mechanisms of toxicity, and identify specific gene expression profiles that can provide biomarkers of exposure and risk. Compared to previous approaches to measuring molecular changes caused by toxicants, toxicogenomic technologies can improve environmental risk assessment while reducing animal studies. We discuss the prospects and limitations of converting omic datasets into valuable information, focusing on assessing the risks of mixed toxic substances to the environment and human health.

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Impact of exposure to atmospheric particulate matter in human skin-derived fibroblast cells: A metabolomics approach for the class of amino acids based on GC×GC-Q-TOFMS/MS

Rodrigues,

Menezes,

Gomes

et al. 2024

Journal of Hazardous Materials

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Genomic Approach to the Assessment of Adverse Effects of Particulate Matters on Skin Cancer and Other Disorders and Underlying Molecular Mechanisms

Cited by 4 publications

References 77 publications

Particulate matter 10 induces oxidative stress and apoptosis in rhesus macaques skin fibroblast

Particulate matter 10 induces oxidative stress and apoptosis in rhesus macaques skin fibroblast

Perspectives on the Use of Toxicogenomics to Assess Environmental Risk

Impact of exposure to atmospheric particulate matter in human skin-derived fibroblast cells: A metabolomics approach for the class of amino acids based on GC×GC-Q-TOFMS/MS

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