Genomic and Transcriptomic Analyses of Bioluminescence Genes in the Enope Squid Watasenia scintillans

Yoshida, Masa‐aki; Imoto, Junichi; Kawai, Yuri; Funahashi, Shigenobu; Minei, Ryuhei; Akizuki, Yuki; Ogura, Atsushi; Nakabayashi, Kazuhiko; Yura, Kei; Ikeo, Kazuho

doi:10.1007/s10126-020-10001-8

Cited by 3 publications

(1 citation statement)

References 68 publications

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“…The luciferase of Watasenia has been found to be ATP-dependent and to exist in a crystalline structure [ 4 , 5 ]. In recent years, there have been attempts to identify Watasenia luciferase through mass spectrometry and transcriptome analysis; however, active luciferase has not yet been purified [ 6 , 7 ]. Watasenia luciferin has been identified and isolated from the light organ in arm photophores [ 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

A New Method for Albuminuria Measurement Using a Specific Reaction between Albumin and the Luciferin of the Firefly Squid Watasenia scintillans

Ishimoto

Okada

Fujisaka

et al. 2022

IJMS

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This study demonstrates that the luciferin of the firefly squid Watasenia scintillans, which generally reacts with Watasenia luciferase, reacted with human albumin to emit light in proportion to the albumin concentration. The luminescence showed a peak wavelength at 540 nm and was eliminated by heat or protease treatment. We used urine samples collected from patients with diabetes to quantify urinary albumin concentration, which is essential for the early diagnosis of diabetic nephropathy. Consequently, we were able to measure urinary albumin concentrations by precipitating urinary proteins with acetone before the reaction with luciferin. A correlation was found with the result of the immunoturbidimetric method; however, the Watasenia luciferin method tended to produce lower albumin concentrations. This may be because the Watasenia luciferin reacts with only intact albumin. Therefore, the quantification method using Watasenia luciferin is a new principle of urinary albumin measurement that differs from already established methods such as immunoturbidimetry and high-performance liquid chromatography.

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Section: Introductionmentioning

confidence: 99%

A New Method for Albuminuria Measurement Using a Specific Reaction between Albumin and the Luciferin of the Firefly Squid Watasenia scintillans

Ishimoto

Okada

Fujisaka

et al. 2022

IJMS

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Enzymatic sulfation of coelenterazine by human cytosolic aryl sulfotransferase SULT1A1: identification of coelenterazine C2-benzyl monosulfate by LC/ESI-TOF-MS

Inouye

Matsuda

Nakamura

2023

Biochemical and Biophysical Research Communications

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Leveraging Short-Read Sequencing to Explore the Genomics of Sepiolid Squid

Heath‐Heckman

Nishiguchi

2021

Integrative and Comparative Biology

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Due to their large size (∼3–5 Gb) and high repetitive content, the study of cephalopod genomes has historically been problematic. However, with the recent sequencing of several cephalopod genomes, including the Hawaiian bobtail squid (Euprymna scolopes), whole-genome studies of these molluscs are now possible. Of particular interest are the sepiolid or bobtail squids, many of which develop photophores in which bioluminescent bacterial symbionts reside. The variable presence of the symbiosis throughout the family allows us to determine regions of the genome that are under selection in symbiotic lineages, potentially providing a mechanism for identifying genes instrumental in the evolution of these mutualistic associations. To this end, we have used high-throughput sequencing to generate sequence from five bobtail squid genomes, four of which maintain symbioses with luminescent bacteria (E. hyllebergi, E. albatrossae, E. scolopes and Rondeletiola minor), and one of which does not (Sepietta neglecta). When we performed K-mer based heterozygosity and genome size estimations, we found that the Euprymna genus has a higher predicted genome size than other bobtail squid (∼ 5 Gb as compared to ∼ 4 Gb) and lower genomic heterozygosity. When we analyzed the repetitive content of the genomes, we found that genomes in the genus Euprymna appear to have recently acquired a significant quantity of LINE elements that are not found in its sister genus Rondeletiola or the closely related Sepietta. Using Abyss-2.0 and then Chromosomer with the published E. scolopes genome as a reference, we generated E. hyllebergi and E. albatrossae genomes of 1.54–1.57 Gb in size, but containing over 78–81% of eukaryotic single-copy othologs. The data we have generated will enable future whole-genome comparisons between these species to determine gene and regulatory content that differs between symbiotic and non-symbiotic lineages, as well as genes associated with symbiosis that are under selection.

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Genomic and Transcriptomic Analyses of Bioluminescence Genes in the Enope Squid Watasenia scintillans

Cited by 3 publications

References 68 publications

A New Method for Albuminuria Measurement Using a Specific Reaction between Albumin and the Luciferin of the Firefly Squid Watasenia scintillans

A New Method for Albuminuria Measurement Using a Specific Reaction between Albumin and the Luciferin of the Firefly Squid Watasenia scintillans

Enzymatic sulfation of coelenterazine by human cytosolic aryl sulfotransferase SULT1A1: identification of coelenterazine C2-benzyl monosulfate by LC/ESI-TOF-MS

Leveraging Short-Read Sequencing to Explore the Genomics of Sepiolid Squid

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