Genomic and serological detection of bat coronavirus from bats in the Philippines

Tsuda, Shumpei; Watanabe, Shumpei; Masangkay, Joseph S.; Mizutani, Tetsuya; Alviola, Phillip A.; Ueda, Naoya; Iha, Koichiro; Taniguchi, Satoshi; Fujii, H.; Kato, Kentaro; Horimoto, Taisuke; Kyuwa, Shigeru; Yoshikawa, Yasuhiro; Akashi, Hiroomi

doi:10.1007/s00705-012-1410-z

Cited by 27 publications

(33 citation statements)

References 20 publications

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“…In two species with relatively large sample sizes (Miniopterus australis and M. schreibersii in south-east Queensland), 25% of 154 individuals and 27% of 238 individuals, respectively, were positive for coronavirus RNA. Studies elsewhere have shown similar prevalence rates (Tsuda et al 2012;Suzuki et al 2014); others have reported much lower prevalence (Anthony et al 2013). Seroprevalence in our study was also high in some populations, including those that were negative for viral RNA.…”

Section: Discussionsupporting

confidence: 81%

“…In some cases, the limited sample size may have constrained detection of viral RNA in the sampled population. Although there have been fewer serological studies than molecular studies in bats, others have also found high coronavirus antibody prevalence in some bat species, albeit using a different serological assay (Tsuda et al 2012). Indeed, given that our ELISA was based on SARS-CoV antigens, it may be that broader coronavirus seroprevalence is underestimated.…”

Section: Discussionmentioning

confidence: 94%

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Coronavirus Infection and Diversity in Bats in the Australasian Region

et al. 2016

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Following the SARS outbreak, extensive surveillance was undertaken globally to detect and identify coronavirus diversity in bats. This study sought to identify the diversity and prevalence of coronaviruses in bats in the Australasian region. We identified four different genotypes of coronavirus, three of which (an alphacoronavirus and two betacoronaviruses) are potentially new species, having less than 90% nucleotide sequence identity with the most closely related described viruses. We did not detect any SARS-like betacoronaviruses, despite targeting rhinolophid bats, the putative natural host taxa. Our findings support the virus-host co-evolution hypothesis, with the detection of Miniopterus bat coronavirus HKU8 (previously reported in Miniopterus species in China, Hong Kong and Bulgaria) in Australian Miniopterus species. Similarly, we detected a novel betacoronavirus genotype from Pteropus alecto which is most closely related to Bat coronavirus HKU9 identified in other pteropodid bats in China, Kenya and the Philippines. We also detected possible cross-species transmission of bat coronaviruses, and the apparent enteric tropism of these viruses. Thus, our findings are consistent with a scenario wherein the current diversity and host specificity of coronaviruses reflects co-evolution with the occasional host shift.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 94%

Coronavirus Infection and Diversity in Bats in the Australasian Region

et al. 2016

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“…A significant diversity of CoVs was found in pteropodids both in Cambodia and Lao PDR. Overall, CoV detection rates (6.5% in Lao PDR and 4.85% in Cambodia) were in the same range as those found in Thailand (7%) (Wacharapluesadee et al, 2015), but lower than those reported in Hong Kong (12%) or Philippines (29.6%) (Tsuda et al, 2012;Woo et al, 2006). Interestingly, for the first time, coronaviruses were detected in Megaerops niphanae and Myotis horsfieldii.…”

Section: Discussionsupporting

confidence: 55%

“…Bats have been identified as the natural host of the SARS-CoV, , and recently, the NeoCoV from the clade the Middle Eastern Respiratory Syndrome (MERS-CoV) was detected in a sub-Saharan bat (Neoromicia capensis) besides its camel host (Corman et al, 2014). A growing number of coronaviruses have been detected in bats since the SARS-CoV outbreak (Chu et al, 2006(Chu et al, , 2008Lau et al, 2007Lau et al, , 2010Lau et al, , 2012Watanabe et al, 2010;Gouilh et al, 2011;Tsuda et al, 2012;Wacharapluesadee et al, 2013Wacharapluesadee et al, , 2015Anindita et al, 2015;Xu et al, 2016, Kim et al, 2016 including a high diversity of coronaviruses, recently detected in five Thai provinces neighboring Cambodia (Wacharapluesadee et al, 2013(Wacharapluesadee et al, , 2015.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of coronaviruses in bats in Lao PDR and Cambodia

Lacroix

Duong

Hul

et al. 2017

Infection, Genetics and Evolution

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South-East Asia is a hot spot for emerging zoonotic diseases, and bats have been recognized as hosts for a large number of zoonotic viruses such as Severe Acute Respiratory Syndrome (SARS), responsible for acute respiratory syndrome outbreaks. Thus, it is important to expand our knowledge of the presence of viruses in bats which could represent a risk to humans. Coronaviruses (CoVs) have been reported in bat species from Thailand, China, Indonesia, Taiwan and the Philippines. However no such work was conducted in Cambodia or Lao PDR. Between 2010 and 2013, 1965 bats were therefore sampled at interfaces with human populations in these two countries. They were tested for the presence of coronavirus by consensus reverse transcription-PCR assay. A total of 93 samples (4.7%) from 17 genera of bats tested positive. Sequence analysis revealed the presence of potentially 37 and 56 coronavirus belonging to alpha-coronavirus (αCoV) and beta-CoV (βCoV), respectively. The βCoVs group is known to include some coronaviruses highly pathogenic to human, such as SARS-CoV and MERS-CoV. All coronavirus sequences generated from frugivorous bats (family Pteropodidae) (n=55) clustered with other bat βCoVs of lineage D, whereas one coronavirus from Pipistrellus coromandra fell in the lineage C of βCoVs which also includes the MERS-CoV. αCoVs were all detected in various genera of insectivorous bats and clustered with diverse bat αCoV sequences previously published. A closely related strain of PEDV, responsible for severe diarrhea in pigs (PEDV-CoV), was detected in 2 Myotis bats. We highlighted the presence and the high diversity of coronaviruses circulating in bats from Cambodia and Lao PDR. Three new bat genera and species were newly identified as host of coronaviruses, namely Macroglossus sp., Megaerops niphanae and Myotis horsfieldii.

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“…These ectoparasites are of veterinary importance as potential vectors that enable pathogens and potentially pathogenic microorganisms to bypass barriers such as fur and skin (Messenger et al, 2003;Dick and Patterson, 2006). In the Philippines, bats were recently shown to harbour parasitic protozoans (Murakoshi et al, 2016) and viruses (Watanabe et al, 2010;Taniguchi et al, 2011;Tsuda et al, 2012), although their zoonosis are still indefinite. Bat flies are also reported to harbour fungi belonging to Order Laloulbeniales (Blackwell, 1980;Marshall, 1982).…”

Section: Introductionmentioning

confidence: 99%

A contribution to the ectoparasite fauna of bats (Mammalia: Chiroptera) in Mindoro Island, Philippines: I. Blood sucking Diptera (Nycteribiidae, Streblidae) and Siphonaptera (Ischnopsyllidae)

Alvarez

Lit

Alviola

et al. 2016

Int. J. Trop. Insect Sci.

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New data on bat ectoparasites from Mindoro Island, Philippines are reported. Eighty-three individuals of ectoparasitic insects representing seven species of Nycteribiidae and five species of Streblidae (both Diptera), and one species of Ischnopsyllidae (Siphonaptera) were recorded from 11 bat species captured in Naujan Lake National Park, Mindoro Oriental Province, Philippines. Raymondia pagodarum is a new record for the country. Eight species are also newly recorded for Mindoro Island, including Cyclopodia garrula, Leptocyclopodia ferrarii mabuhai, Megastrebla parvior, Brachytarsina amboinensis, B. werneri, R. pagodarum, R. pseudopagodarum and Thaumapsylla longiforceps. Five species are newly documented on various hosts: C. horsfieldi on Pteropus pumilus, M. parvior on Macroglossus minimus, B. amboinensis on Hipposideros diadema, B. werneri on Rhinolophus arcuatus and R. pagodarum on Hipposideros bicolor.

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Genomic and serological detection of bat coronavirus from bats in the Philippines

Cited by 27 publications

References 20 publications

Coronavirus Infection and Diversity in Bats in the Australasian Region

Coronavirus Infection and Diversity in Bats in the Australasian Region

Genetic diversity of coronaviruses in bats in Lao PDR and Cambodia

A contribution to the ectoparasite fauna of bats (Mammalia: Chiroptera) in Mindoro Island, Philippines: I. Blood sucking Diptera (Nycteribiidae, Streblidae) and Siphonaptera (Ischnopsyllidae)

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