Genomic and Proteomic Profiling of Responses to Toxic Metals in Human Lung Cells

Andrew, Angeline S.; Warren, Amy J.; Barchowsky, Aaron; Temple, Kaili A.; Klei, Linda R.; Soucy, Nicole V.; O’Hara, Kimberley A.; Hamilton, Joshua W.

doi:10.1289/ehp.6249

Cited by 54 publications

(70 citation statements)

References 23 publications

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“…Previous studies showed that the expression of HSP90 at the transcriptional level varied greatly in different species responding to different heavy metal stress. For instance, HSP90a gene expression in human lung cells was up-regulated by As (5 or 50 lmol/L), while down-regulated by heavy metals such as Cd (3 lmol/L), Ni (3 lg/cm 2 ), and Cr (10 lmol/L) (Andrew et al 2003). HSP90a in common carp liver was up-regulated when the fish were kept in water containing 10 mg/L Cd, while in the kidney, it was up-regulated and reached a peak then down-regulated (Hermesz et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Cloning and characterization of the HSP90 beta gene from Tanichthys albonubes Lin (Cyprinidae): effect of copper and cadmium exposure

Liu

Chen

Jing

et al. 2011

Fish Physiol Biochem

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Heat shock protein 90 (HSP90) is a highly conserved molecular chaperone important in the maturation of a broad spectrum of proteins. In order to evaluate the effect of copper (Cu(2+)) and cadmium (Cd(2+)) on the expression of HSP90 from Tanichthys albonubes (designated TaHSP90), the full-length complementary DNA (cDNA) of TaHSP90 was cloned using reverse transcription PCR and rapid amplification of cDNA ends (RACE) techniques. A 2,687-bp sequence was sequenced and consisted of an open reading frame (ORF) of 2,181 bp encoding a polypeptide of 727 amino acids with five HSP90 family signatures. Homologous analysis revealed that TaHSP90 gene shared high similarity with other known HSP90 genes and belonged to HSP90β subtype. Fluorescent real-time quantitative PCR was used to examine the expression pattern of TaHSP90β mRNA in different tissues (liver, muscle, gill, fin, eye, ovary, intestine and brain), and the result indicated that TaHSP90β was widely expressed in all examined tissues at different levels. Sensitivity of TaHSP90β to copper and cadmium was examined by exposing fish to different concentrations of Cu(2+) (0, 13.50 and 27.00 μg/L) and Cd(2+) (0, 1.15, 2.31 mg/L) for 24, 48, 72 and 96 h, respectively. The copper treatment induced TaHSP90β expression slight increase only at 24 and 48 h, while cadmium treatment caused slight down-regulation of TaHSP90β only 72 and 96 h. Our data suggest that the cloning and expression analysis of T. albonubes HSP90β gene provided useful molecular information of T. albonubes responses in stress conditions and potential ways to monitor the chronic stressors in T. albonubes culture environments.

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Section: Discussionmentioning

confidence: 99%

Cloning and characterization of the HSP90 beta gene from Tanichthys albonubes Lin (Cyprinidae): effect of copper and cadmium exposure

Liu

Chen

Jing

et al. 2011

Fish Physiol Biochem

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“…Thangaradjou et al [39] reported interspecific and intraspecific differences in the accumulation of different metals (Mn, Al, Fe, Cr, Cu, Zn, Pb, Cd, Co and Ni) in seagrasses of Andaman Islands. The presence of heavy metals in food and medicinal plants may cause severe health problems including renal failure, chronic toxicity and liver damage [44][45][46]. According to the World Health Organization [47], the concentration of heavy metals must be controlled in medicinal plants in order to meet the quality assurance.…”

Section: Introductionmentioning

confidence: 99%

Chemometric Studies of Multielemental Composition of Few Seagrasses from Gulf of Mannar, India

Rengasamy

Rajasekaran

Anantharaman

2010

Biol Trace Elem Res

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This paper discusses the determination of minerals content (cadmium, cobalt, chromium, copper, nickel, lead, manganese, magnesium, iron, zinc, sodium, potassium and calcium) of six seagrass samples, Enhalus acoroides, Thalassia hemprichii, Halodule pinifolia, Syringodium isoetifolium, Cymodocea serrulata and Cymodocea rotundata using inductively coupled plasma optical emission spectrophotometry and flame photometer. Principal component analysis (PCA) and hierarchical cluster analysis revealed different mineral compositions of the seagrass samples. Among the 13 elements investigated, Ni 1.513, Na 690.167 and Ca 220.333; Cr 3.957; Mn 23.427, Zn 17.593 and Fe 156.567; Cd 0.357, Co 0.431, Pb 2.040, Mg 912.733 and K 300.9; Cu 7.8 mg/kg dry weight, respectively, were found at high concentrations in E. acoroides; T. hemprichii; H. pinifolia; S. isoetifolium and C. rotundata. PCA analysis confirmed the presence of three components with 91.28% of the total variance. The toxic elements Pb, Cr and Cd were also found in all six seagrasses, although the concentrations were below the permissible limits proposed by the World Health Organization.

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“…Consequently, arsenic may act on stem or progenitor cells of the bronchial epithelium by altering the expression of genes involved in key regulatory pathways. Indeed, arsenic has been shown to inhibit protein tyrosine and serine/threonine phosphatases, regulatory enzymes that often carry a thiol group in their active center, and to alter the expression of many kinases implicated in signaling pathways (Andrew et al 2003;Cavigelli et al 1996;Huang et al 1995;Tapio et al 2005). In line with this, large-scale expression analysis revealed that speciWc lung developmental pathways are reactivated in NSCLC (Borczuk et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Major histopathological patterns of lung cancer related to arsenic exposure in German uranium miners

Taeger

Johnen

Wiethege

et al. 2008

Int Arch Occup Environ Health

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Genomic and Proteomic Profiling of Responses to Toxic Metals in Human Lung Cells

Cited by 54 publications

References 23 publications

Cloning and characterization of the HSP90 beta gene from Tanichthys albonubes Lin (Cyprinidae): effect of copper and cadmium exposure

Cloning and characterization of the HSP90 beta gene from Tanichthys albonubes Lin (Cyprinidae): effect of copper and cadmium exposure

Chemometric Studies of Multielemental Composition of Few Seagrasses from Gulf of Mannar, India

Major histopathological patterns of lung cancer related to arsenic exposure in German uranium miners

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