Genomic and proteomic analysis of Tausonia pullulans reveals a key role for a GH15 glucoamylase in starch hydrolysis

Trochine, Andrea; Bellora, Nicolás; Nizovoy, Paula; Durán, Rosario; Greif, Gonzalo; García, Virginia de; Batthyány, Carlos; Robello, Carlos; Libkind, Diego

doi:10.1007/s00253-022-12025-7

Cited by 7 publications

(3 citation statements)

References 68 publications

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“…This yeast is commonly found in plant material and soil samples and is considered a psychrotolerant, with a set of produced cold-active enzymes, such as lignin-degrading enzymes or lipases. It also harbors a collection of hydrolytic enzymes, whose activities provide nutrients for fungal growth from various substrates [103,104]. Basidiomycota fungi are generally found in association with substrates rich in nutrients, such as wood and manure, within underground settings.…”

Section: Discussionmentioning

confidence: 99%

Soil and Sediments in Natural Underground Ecosystems as a Source of Culturable Micromycetes: A Case Study of the Brestovská Cave (Western Tatras, Slovakia)

Ogórek,

Borzęcka,

Spychała

et al. 2024

Applied Sciences

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Soil and sediment host microorganisms are able to survive in extremely resource-limited environments. Therefore, more and more attention is being paid to cave sediments as a reservoir of microbiota. The aim of this study is the speleomycological evaluation of the culturable soil and sediment fungal communities in the Brestovská Cave. To explore the origins of fungi, speleomycological studies were conducted both inside and outside the cave under investigation. Additionally, two incubation temperatures (5 and 24 °C) were used to increase the species spectrum of isolated fungi. To achieve the most accurate species identification, we combined an assessment of morphological characteristics of the isolates with molecular sequencing (ITS, internal transcribed spacer). Twenty different species were found and the most frequent was Penicillium commune, followed by Trichosporiella cerebriformis and Pseudogymnoascus pannorum. To our knowledge, our study has enabled the first identification of fungal species such as Penicillium swiecicki, Cephalotrichum hinnuleum, Cosmpospora berkeleyana, Lecythophora hoffmannii, Ambomucor seriatoinflatus, and Mortierella minutissima in underground sites. Our data showed that the abundance and composition of the fungal community varied between the indoor and outdoor samples and thus from the entrance and less visited sites deeper in the cave.

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Section: Discussionmentioning

confidence: 99%

Soil and Sediments in Natural Underground Ecosystems as a Source of Culturable Micromycetes: A Case Study of the Brestovská Cave (Western Tatras, Slovakia)

Ogórek,

Borzęcka,

Spychała

et al. 2024

Applied Sciences

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“…Few crop- or field-specific eukaryotic core nSVs were present in the nSVs; however, five eukaryotic SVs (SV_2, 4, 28, 43, and 63) were not only common nSVs but also core nSVs in more than two networks (Supplementary Table S10 ). Two abundant SVs (SV_2 and SV_4) were derived from the Basidiomycota Tausonia and Solicoccozyma genera that respectively contains enzyme-producing yeasts 65 and plant-growth promoting microorganisms 66 . SV_28, SV_43, and SV_63 were assigned to the Spongomonas and Heteromita genera in Cercozoa and the Colpoda genus in Ciliophora respectively.…”

Section: Discussionmentioning

confidence: 99%

Distinct prokaryotic and eukaryotic communities and networks in two agricultural fields of central Japan with different histories of maize–cabbage rotation

Kenmotsu,

Masuma,

Murakami

et al. 2023

Sci Rep

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Crop rotation is an important agricultural practice for homeostatic crop cultivation. Here, we applied high-throughput sequencing of ribosomal RNA gene amplicons to investigate soil biota in two fields of central Japan with different histories of maize–cabbage rotation. We identified 3086 eukaryotic and 17,069 prokaryotic sequence variants (SVs) from soil samples from two fields rotating two crops at three different growth stages. The eukaryotic and prokaryotic communities in the four sample groups of two crops and two fields were clearly distinguished using β-diversity analysis. Redundancy analysis showed the relationships of the communities in the fields to pH and nutrient, humus, and/or water content. The complexity of eukaryotic and prokaryotic networks was apparently higher in the cabbage-cultivated soils than those in the maize-cultivated soils. The node SVs (nSVs) of the networks were mainly derived from two eukaryotic phyla: Ascomycota and Cercozoa, and four prokaryotic phyla: Pseudomonadota, Acidobacteriota, Actinomycetota, and Gemmatimonadota. The networks were complexed by cropping from maize to cabbage, suggesting the formation of a flexible network under crop rotation. Ten out of the 16 eukaryotic nSVs were specifically found in the cabbage-cultivated soils were derived from protists, indicating the potential contribution of protists to the formation of complex eukaryotic networks.

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“…When these approaches are integrated, they shed light on the critical aspects of parasite–host interactions and parasite biology. Each addition adds to our understanding of the transcriptome profiles, genomic architecture, proteomic targets, and chromatin landscape organization of T. cruzi [ 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Exploring Peripheral Blood-Derived Extracellular Vesicles as Biomarkers: Implications for Chronic Chagas Disease with Viral Infection or Transplantation

Madeira,

Meneghetti,

Lozano

et al. 2024

Microorganisms

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Extracellular vesicles (EVs) are lipid bilayer envelopes that encapsulate cell-specific cargo, rendering them promising biomarkers for diverse diseases. Chagas disease, caused by the parasite Trypanosoma cruzi, poses a significant global health burden, transcending its initial epicenter in Latin America to affect individuals in Europe, Asia, and North America. In this study, we aimed to characterize circulating EVs derived from patients with chronic Chagas disease (CCD) experiencing a reactivation of acute symptoms. Blood samples collected in EDTA were processed to isolate plasma and subsequently subjected to ultracentrifugation for particle isolation and purification. The EVs were characterized using a nanoparticle tracking analysis and enzyme-linked immunosorbent assay (ELISA). Our findings revealed distinctive differences in the size, concentration, and composition of EVs between immunosuppressed patients and those with CCD. Importantly, these EVs play a critical role in the pathophysiology of Chagas disease and demonstrate significant potential as biomarkers in the chronic phase of the disease. Overall, our findings support the potential utility of the CL-ELISA assay as a specific sensitive tool for detecting circulating EVs in chronic Chagasic patients, particularly those with recurrent infection following an immunosuppressive treatment or with concurrent HIV and Chagas disease. Further investigations are warranted to identify and validate the specific antigens or biomarkers responsible for the observed reactivity in these patient groups, which may have implications for diagnosis, the monitoring of treatment, and prognosis.

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Genomic and proteomic analysis of Tausonia pullulans reveals a key role for a GH15 glucoamylase in starch hydrolysis

Cited by 7 publications

References 68 publications

Soil and Sediments in Natural Underground Ecosystems as a Source of Culturable Micromycetes: A Case Study of the Brestovská Cave (Western Tatras, Slovakia)

Soil and Sediments in Natural Underground Ecosystems as a Source of Culturable Micromycetes: A Case Study of the Brestovská Cave (Western Tatras, Slovakia)

Distinct prokaryotic and eukaryotic communities and networks in two agricultural fields of central Japan with different histories of maize–cabbage rotation

Exploring Peripheral Blood-Derived Extracellular Vesicles as Biomarkers: Implications for Chronic Chagas Disease with Viral Infection or Transplantation

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