Genomic and lipidomic actions of nandrolone on detached rotator cuff muscle in sheep

Flück, Martin; Ruoss, Severin; Möhl, Christoph B.; Valdivieso, Paola; Benn, Mario C.; Rechenberg, Brigitte von; Laczkó, Endre; Hu, Jingyu; Wieser, Karl; Meyer, Dominik C.; Gerber, Christian

doi:10.1016/j.jsbmb.2016.08.005

Cited by 18 publications

(30 citation statements)

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“…Equal loading and blotting was verified via signal intensity of the actin band on the Ponceau S-stained nitrocellulose membrane; after blotting signal intensity was assessed from background-corrected band intensities using PxI system (Syngene) as described. 52 All blots derived from the same experiment and were processed in parallel.…”

Section: Methodsmentioning

confidence: 99%

Sarcolab pilot study into skeletal muscle’s adaptation to long-term spaceflight

et al. 2018

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Spaceflight causes muscle wasting. The Sarcolab pilot study investigated two astronauts with regards to plantar flexor muscle size, architecture, and function, and to the underlying molecular adaptations in order to further the understanding of muscular responses to spaceflight and exercise countermeasures. Two crew members (A and B) spent 6 months in space. Crew member A trained less vigorously than B. Postflight, A showed substantial decrements in plantar flexor volume, muscle architecture, in strength and in fiber contractility, which was strongly mitigated in B. The difference between these crew members closely reflected FAK-Y397 abundance, a molecular marker of muscle’s loading history. Moreover, crew member A showed downregulation of contractile proteins and enzymes of anaerobic metabolism, as well as of systemic markers of energy and protein metabolism. However, both crew members exhibited decrements in muscular aerobic metabolism and phosphate high energy transfer. We conclude that countermeasures can be effective, particularly when resistive forces are of sufficient magnitude. However, to fully prevent space-related muscular deterioration, intersubject variability must be understood, and intensive exercise countermeasures programs seem mandatory. Finally, proteomic and metabolomic analyses suggest that exercise benefits in space may go beyond mere maintenance of muscle mass, but rather extend to the level of organismic metabolism.

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Section: Methodsmentioning

confidence: 99%

Sarcolab pilot study into skeletal muscle’s adaptation to long-term spaceflight

et al. 2018

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“…Further complicating the evaluation of SCs in the context of RC disease are other potential environmental contributors to SC dysfunction known to occur in the torn RC. The torn RC environment demonstrates altered expression of soluble factors as well as increased inflammation and oxidative stress, which are all potent mediators of SC function that may be exacerbated by muscle injury. Pro‐inflammatory signaling and oxidative stress stimulate SC proliferation, but blocks differentiation, and self‐renewal via the TNF/TWEAK/NF‐κB and Notch signaling axes, while anti‐inflammatory signals aid in differentiation (Fig.…”

Section: Can Satellite Cells Maintain Muscle Homeostasis Post‐injury?mentioning

confidence: 99%

The role of mechanobiology in progression of rotator cuff muscle atrophy and degeneration

Gibbons

Singh

Engler

et al. 2017

Journal Orthopaedic Research

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Rotator cuff (RC) muscles undergo several detrimental changes following mechanical unloading resulting from RC tendon tear. In this review, we highlight the pathological causes and consequences of mechanical alterations at the whole muscle, muscle fiber, and muscle resident cell level as they relate to RC disease progression. In brief, the altered mechanical loads associated with RC tear lead to architectural, structural, and compositional changes at the whole-muscle and muscle fiber level. At the cellular level, these changes equate to direct disruption of mechanobiological signaling, which is exacerbated by mechanically regulated biophysical and biochemical changes to the cellular and extra-cellular environment (also known as the stem cell "niche"). Together, these data have important implications for both pre-clinical models and clinical practice. In pre-clinical models, it is important to recapitulate both the atrophic and degenerative muscle loss found in humans using clinically relevant modes of injury. Clinically, understanding the mechanics and underlying biology of the muscle will impact both surgical decision-making and rehabilitation protocols, as interventions that may be good for atrophic muscle will have a detrimental effect on degenerating muscle, and vice versa. © 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:546-556, 2018.

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“…Each gel included both samples from 1 sheep for each of the 3 interventions in adjacent lanes. Proteins of interest were detected by immunoblotting with specific antibodies based on enhanced chemiluminescence, as described previously The following primary antibodies were used: Tenascin‐C antibody B28.13 (gift from Prof. R. Chiquet‐Ehrismann, Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland), laminin antibody (PA1‐32130, Thermo Fisher, Life Technologies Europe, Zug, Switzerland) recognizing the α2 (150 kDa) and β1γ1 (200 kDa) subumits, PPARG antibody (LS‐C178333; Lifespan Biosciences, LabForce AG, Nunningen, Switzerland), myogenin antibody F5D (SC‐12732; Santa Cruz Biotechnology, LabForce AG, Nunningen, Switzerland), myoD antibody (SC‐304; Santa Cruz Biotechnology, LabForce AG), CaMKII antibody (No. 611293; BD Biosciences, Allschwil, Switzerland), and skeletal α actin (A‐2172; Sigma, Buchs, Switzerland).…”

Section: Methodsmentioning

confidence: 99%