Genomic and In Vitro Phenotypic Comparisons of Epidemic and Non-Epidemic Getah Virus Strains

Mohamed-Romai-Noor, Noor-Adila; Sam, Sing‐Sin; Teoh, Boon-Teong; Hamim, Zur-Raiha; AbuBakar, Sazaly

doi:10.3390/v14050942

Cited by 3 publications

(1 citation statement)

References 38 publications

(54 reference statements)

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“…The Getah virus (GETV), is an alphavirus that is transmitted by mosquitoes and belongs to the Togaviridae family 1 . Currently, the GETV is widely reported in Southeast Asia.…”

Section: Introductionmentioning

confidence: 99%

The roles of 6K protein on Getah virus replication and pathogenicity

Meng,

Mou,

Zhang

et al. 2023

Journal of Medical Virology

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Alphavirus is a type of arbovirus that can infect both humans and animals. The amino acid sequence of the 6K protein, being one of the structural proteins of the alphavirus, is not conserved. Deletion of this protein will result in varying effects on different alphaviruses. Our study focuses on the function of the Getah virus (GETV) 6K protein in infected cells and mice. We successfully constructed infectious clone plasmids and created resulting viruses (rGETV and rGETV‐Δ6K). Our comprehensive microscopic analysis revealed that the 6 K protein mainly stays in the endoplasmic reticulum. In addition, rGETV‐Δ6K has lower thermal stability and sensitivity to temperature than GETV. Although the deletion of the 6K protein does not reduce virion production in ST cells, it affects the release of virions from host cells by inhibiting the process of E2 protein transportation to the plasma membrane. Subsequent in vivo testing demonstrated that neonatal mice infected with rGETV‐Δ6K had a lower virus content, less significant pathological changes in tissue slices, and milder disease than those infected with the wild‐type virus. Our results indicate that the 6K protein effectively reduces the viral titer by influencing the release of viral particles. Furthermore, the 6K protein play a role in the clinical manifestation of GETV disease.

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“…The Getah virus (GETV), is an alphavirus that is transmitted by mosquitoes and belongs to the Togaviridae family 1 . Currently, the GETV is widely reported in Southeast Asia.…”

Section: Introductionmentioning

confidence: 99%

The roles of 6K protein on Getah virus replication and pathogenicity

Meng,

Mou,

Zhang

et al. 2023

Journal of Medical Virology

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Development of a monoclonal antibody specifically recognizing a linear epitope on the capsid protein of the emerging Group III Getah virus

Zhou,

Ren,

Liu

et al. 2024

Virology

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Evaluating the mosquito host range of Getah virus and the vector competence of selected medically important mosquitoes in Getah virus transmission

et al. 2023

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Background The Getah virus (GETV) is a mosquito-borne Alphavirus (family Togaviridae) that is of significant importance in veterinary medicine. It has been associated with major polyarthritis outbreaks in animals, but there are insufficient data on its clinical symptoms in humans. Serological evidence of GETV exposure and the risk of zoonotic transmission makes GETV a potentially medically relevant arbovirus. However, minimal emphasis has been placed on investigating GETV vector transmission, which limits current knowledge of the factors facilitating the spread and outbreaks of GETV. Methods To examine the range of the mosquito hosts of GETV, we selected medically important mosquitoes, assessed them in vitro and in vivo and determined their relative competence in virus transmission. The susceptibility and growth kinetics of GETVs in various mosquito-derived cell lines were also determined and quantified using plaque assays. Vector competency assays were also conducted, and quantitative reverse transcription-PCR and plaque assays were used to determine the susceptibility and transmission capacity of each mosquito species evaluated in this study. Results GETV infection in all of the investigated mosquito cell lines resulted in detectable cytopathic effects. GETV reproduced the fastest in Culex tritaeniorhynchus- and Aedes albopictus-derived cell lines, as evidenced by the highest exponential titers we observed. Regarding viral RNA copy numbers, mosquito susceptibility to infection, spread, and transmission varied significantly between species. The highest vector competency indices for infection, dissemination and transmission were obtained for Cx. tritaeniorhynchus. This is the first study to investigate the ability of Ae. albopictus and Anopheles stephensi to transmit GETV, and the results emphasize the role and capacity of other mosquito species to transmit GETV upon exposure to GETV, in addition to the perceived vectors from which GETV has been isolated in nature. Conclusions This study highlights the importance of GETV vector competency studies to determine all possible transmission vectors, especially in endemic regions. Graphical Abstract

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Genomic and In Vitro Phenotypic Comparisons of Epidemic and Non-Epidemic Getah Virus Strains

Cited by 3 publications

References 38 publications

The roles of 6K protein on Getah virus replication and pathogenicity

The roles of 6K protein on Getah virus replication and pathogenicity

Development of a monoclonal antibody specifically recognizing a linear epitope on the capsid protein of the emerging Group III Getah virus

Evaluating the mosquito host range of Getah virus and the vector competence of selected medically important mosquitoes in Getah virus transmission

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