Genomic and functional evolution of the Drosophila melanogaster sperm proteome

Dorus, Steve; Busby, Scott A.; Gerike, Ursula; Shabanowitz, Jeffrey; Hunt, Donald F.; Karr, Timothy L.

doi:10.1038/ng1915

Cited by 246 publications

(339 citation statements)

References 27 publications

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“…First, at least four of them are expressed at the mRNA level and collectively account for one-fifth of the functional Mst77F-like mRNA. The Mst77Y mRNAs most likely are translated, since they are similar to the Mst77F mRNA; unfortunately none of these proteins (Mst77F or Mst77Y) were detected in the D. melanogaster sperm proteome (Dorus et al 2006) or in the whole male reproductive system (Takemori and Yamamoto 2009), so we cannot prove (or disprove) the expression of the Mst77Y genes at the protein level.…”

Section: Discussionmentioning

confidence: 99%

Functional Copies of the Mst77F Gene on the Y Chromosome of Drosophila melanogaster

et al. 2010

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The Y chromosome of Drosophila melanogaster has ,20 protein-coding genes. These genes originated from the duplication of autosomal genes and have male-related functions. In 1993, Russell and Kaiser found three Y-linked pseudogenes of the Mst77F gene, which is a testis-expressed autosomal gene that is essential for male fertility. We did a thorough search using experimental and computational methods and found 18 Y-linked copies of this gene (named Mst77Y-1-Mst77Y-18). Ten Mst77Y genes encode defective proteins and the other eight are potentially functional. These eight genes produce $20% of the functional Mst77F-like mRNA, and molecular evolutionary analysis shows that they evolved under purifying selection. Hence several Mst77Y genes have all the features of functional genes. Mst77Y genes are present only in D. melanogaster, and phylogenetic analysis confirmed that the duplication is a recent event. The identification of functional Mst77Y genes reinforces the previous finding that gene gains play a prominent role in the evolution of the Drosophila Y chromosome.

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Section: Discussionmentioning

confidence: 99%

Functional Copies of the Mst77F Gene on the Y Chromosome of Drosophila melanogaster

et al. 2010

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“…The recently reported D. melanogaster sperm proteome has revealed that there has been a significant proportion of duplications among sperm protein genes (Dorus et al 2006), which could fuel rapid evolution across species. However, in comparisons between D. melanogaster and D. simulans, Dorus et al (2006) found that more than half of the sperm proteome genes had d N values ,0.01, suggesting that selection is constraining the evolution of structurally and functionally important genes.…”

Section: Discussionmentioning

confidence: 99%

Evolution in the Fast Lane: Rapidly Evolving Sex-Related Genes in Drosophila

et al. 2007

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A large portion of the annotated genes in Drosophila melanogaster show sex-biased expression, indicating that sex and reproduction-related genes (SRR genes) represent an appreciable component of the genome. Previous studies, in which subsets of genes were compared among few Drosophila species, have found that SRR genes exhibit unusual evolutionary patterns. Here, we have used the newly released genome sequences from 12 Drosophila species, coupled to a larger set of SRR genes, to comprehensively test the generality of these patterns. Among 2505 SRR genes examined, including ESTs with biased expression in reproductive tissues and genes characterized as involved in gametogenesis, we find that a relatively high proportion of SRR genes have experienced accelerated divergence throughout the genus Drosophila. Several testis-specific genes, male seminal fluid proteins (SFPs), and spermatogenesis genes show lineage-specific bursts of accelerated evolution and positive selection. SFP genes also show evidence of lineage-specific gene loss and/ or gain. These results bring us closer to understanding the details of the evolutionary dynamics of SRR genes with respect to species divergence.

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“…One intriguing possibility is that AgOrs act to mediate spermatozoa responses to endogenous signaling molecules. Indeed, several studies have suggested the existence of signaling pathways in insect sperm, including proteomics analyses in Aedes aegypti (18) and Drosophila melanogaster (19), although ORs were not identified in those studies. Importantly, OR expression in male germ cells has been reported for numerous mammalian species (20)(21)(22) and evidence for functional expression of ORs in human and mouse sperm have been described (23)(24)(25)(26)(27), although the requirement for human ORs in ligand recognition and fertilization has been seriously challenged (28).…”

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confidence: 99%

Odorant receptor-mediated sperm activation in disease vector mosquitoes

Pitts

Liu

Zhou

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Insects, such as the malaria vector mosquito, Anopheles gambiae, depend upon chemoreceptors to respond to volatiles emitted from a range of environmental sources, most notably blood meal hosts and oviposition sites. A subset of peripheral signaling pathways involved in these insect chemosensory-dependent behaviors requires the activity of heteromeric odorant receptor (OR) ion channel complexes and ligands for numerous A. gambiae ORs (AgOrs) have been identified. Although AgOrs are expressed in nonhead appendages, studies characterizing potential AgOr function in nonolfactory tissues have not been conducted. In the present study, we explore the possibility that AgOrs mediate responses of spermatozoa to endogenous signaling molecules in A. gambiae. In addition to finding AgOr transcript expression in testes, we show that the OR coreceptor, AgOrco, is localized to the flagella of A. gambiae spermatozoa where Orco-specific agonists, antagonists, and other odorant ligands robustly activate flagella beating in an Orco-dependent process. We also demonstrate Orco expression and Orco-mediated activation of spermatozoa in the yellow fever mosquito, Aedes aegypti. Moreover, we find Orco localization in testes across distinct insect taxa and posit that OR-mediated responses in spermatozoa may represent a general characteristic of insect reproduction and an example of convergent evolution.transcriptomics | cell signaling | gamete | chemical ecology | Culicidae

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Genomic and functional evolution of the Drosophila melanogaster sperm proteome

Cited by 246 publications

References 27 publications

Functional Copies of the Mst77F Gene on the Y Chromosome of Drosophila melanogaster

Functional Copies of the Mst77F Gene on the Y Chromosome of Drosophila melanogaster

Evolution in the Fast Lane: Rapidly Evolving Sex-Related Genes in Drosophila

Odorant receptor-mediated sperm activation in disease vector mosquitoes

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