Genomic and evolutionary features of two AHPND positive Vibrio parahaemolyticus strains isolated from shrimp (Penaeus monodon) of south-west Bangladesh

Ahmmed, Shawon; Khan, A. A.; Eshik, Md. Mostavi Enan; Punom, Nusrat Jahan; Islam, Abul B.M.M.K.; Rahman, Mohammad Shamsur

doi:10.1186/s12866-019-1655-8

Cited by 18 publications

(8 citation statements)

References 50 publications

(64 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our study is in accordance to report by Chosin et al (2015), none of the eight AP-positive AHPND V. parahaemolyticus strains possesses the genes for the conventional virulence factors affecting humans, such as tdh, trh and type III secretion system 2 (14) . In Bangladesh, Ahmmed et al (2019) found tlh, pirA and pirB genes from two AHPND positive V. parahaemolyticus strains isolated from shrimp (Penaeus monodon) (15) . The previous studies in Malaysia showed that the most virulent of the non-cholera vibrios (V. vulnificus) have various virulence factors that facilitate the development of clinical disease (16) .…”

Section: Isolation Of Pathogenic Vibriomentioning

confidence: 99%

Genetic diversity and toxic genes analysis of vibrio spp. isolated from white leg shrimp and marine fishes cultured in Tam G iang lagoon in Thua Thien Hue province, Vietnam

Quảng¹

2020

IJST

View full text Add to dashboard Cite

Objective: This study was done to report the results of genetic diversity and toxic genes analysis of Vibrio pathogen isolated from white leg shrimp and marine fishes cultured in Thua Thien Hue province, Vietnam. Methods/statistical analysis: Pathogen Vibrio spp. were isolated from shrimps and fishes, and were identified by 16S rRNA sequencing. The presence of toxin genes in Vibrio spp. strains were determined through the presence of genes encoding toxic proteins (pirAvp, pir-Bvp, tlh, tdh and trh) based on specific primers for these genes. Genetic diversity of Vibrio strains was analysed by RAPD technique. Findings: A total of 120 Vibrio colonies from shrimps (with Acute Hepatopancreatic Necrosis Disease) and fishes (with hemorrhagic disease) cultured in Tam Giang lagoon in Thua Thien Hue, Vietnam were isolated. Of which, 14/54 strains from shrimps had pirAvp and pirBvp genes and 18/66 strains from fishes had tlh gene, and none of Vibrio strains had tdh and trh genes. Randomly amplified polymorphic DNA (RAPD) analysis of 36 Vibrio samples showed the 148 polymorphic DNA fragments from ten random primers. The genetic diversity is high within studied species. In there, V. parahaemolyticus has the highest diversity level (h=0.1645 and I=0.2695) while V. shilonii is lowest (h=0.0136) and I=0.0207). The degree of genetic differentiation among species is also high (Gst=0.4827). Genetic identity between V. parahaemolyticus and V. vulnificus (0.9545) is highest while between V. shilonii and V. harveyi (0.7416) is lowest. The dendrogram also showed that V. parahaemolyticus is closely related to V. vulnificus whereas V. shilonii and V. harveyi have large distance. Application/improvements: This study is aimed to provide scientific data as the basis for the study and production of rapid diagnostic kits in the future.

show abstract

Section: Isolation Of Pathogenic Vibriomentioning

confidence: 99%

Genetic diversity and toxic genes analysis of vibrio spp. isolated from white leg shrimp and marine fishes cultured in Tam G iang lagoon in Thua Thien Hue province, Vietnam

Quảng¹

2020

IJST

View full text Add to dashboard Cite

show abstract

“…The collected protein concentrations decreased gradually from EF3 to EF6 elution fractions, reaching the lowest in EF6 (17.46 µg/ml). (Ahmmed et al, 2019). The results of genomic analysis of V.…”

Section: Pira Recombinant Antigen Purificationmentioning

confidence: 99%

Cloning and Expression of PirA gene of Vibrio parahaemolyticus Strain K5 Causing Acute Hepatopancreatic Necrosis Disease in Whiteleg Shrimp in E. coli Host Cell

Linh¹,

Nguyen²,

Tran³

et al. 2022

Preprint

View full text Add to dashboard Cite

Background: Acute hepatopancreatic necrosis disease (AHPND), is a bacterial disease of whiteleg shrimp, which has a high mortality rate (100%) and incurs economic losses. Our objective was to identify the genes which lead to cell and organ damage and investigate bioproducts to prevent and treat. Methods: Litopenaeus vannamei shrimp in Thua Thien Hue province, Vietnam were collected from an infected pond and analysed at the Institute of Biotechnology, Hue University. The PirA gene of Vibrio parahaemolyticus strain K5 was isolated and analyzed for nucleotide sequence and paired with the expression vector pQE30. The expression vector was transformed into E. coli strain M15, the PirA recombinant protein was expressed in the form of 6xHis-PirA fusion protein of about 15 kDa. PirA recombinant protein was purified and determined the PirAvp binding ratio, cloning and sequencing of PirA gene from Vibrio parahaemolyticus strain K5 causing AHPND by PCR method with specific primers and molecular weights of PirAvp and the PirAvp complex. Results: PirA gene from Vibrio parahaemolyticus strain K5 was cloned into pGEM-T easy vector (Promega, USA) and screened E. coli TOP10 colonies containing pGEM T easy/PirA recombinant plasmid on LB agar/ampicillin/IPTG/X-Gal medium. PCR showing a band of about 347 bp, matching the size of PirA gene and two nucleotide sequences (BamHI and HindIII). The results showed that PirA gene has a length of 336 bp and similar to PirA gene on GenBank (Code: KU556825.1). The results of protein extracted from E. coli M15 recombinant cells and 6xHis-PirA target protein was collected in elution fractions from EF2 to EF6, showed that the concentration of 6xHis-PirA protein and EF3 elution fraction collected a highest protein concentration (1,586.54 µg/ml). Conclusions: The purified PirA recombinant protein will provide materials for development research to create biological products to prevent and treat AHPND.

show abstract

“…However, further investigations are required to identify/confirm the causative agent of these problems for intensive shrimp farming in Bangladesh. Recent publications suggested AHPND infection in shrimp farms in Bangladesh (Ahmmed et al, 2019).…”

Section: Disease Occurrence and Health Management Practicesmentioning

confidence: 99%

Economic performance characterization of intensive shrimp (Penaeus monodon) farming systems in Bangladesh

Ali¹,

Rahman²,

Jaman³

et al. 2022

Aquaculture Fish & Fisheries

View full text Add to dashboard Cite

Intensive shrimp (Penaeus monodon) production system is relatively new in Bangladesh, and has not yet been adequately described or its viability assessed. The aims of this study were to characterize, assess the economic performance and identify challenges for intensive shrimp farming in Bangladesh. A survey was conducted using a structured questionnaire enumerated between October 2016 to June 2017 with 53 farmers in three districts of Khulna Province, southwest Bangladesh. The surveyed farms were categorized into small, medium and large scale based on the number of culture ponds. The average production, operational costs, gross and net income, net income, and cost-benefit ratios were higher in large farms, followed by medium and small farms.A Cobb-Douglas production function model was used to identify factors influencing shrimp yields, with feed management, health management, pond depth and aeration identified as significant factors. The benefit-cost ratio was higher than 1, indicative of a positive investment efficiency of intensive shrimp farming system for farmers. Major challenges were associated with quality of inputs, high investment, maintenance of biosecurity and disease outbreaks, water quality, limited number of input suppliers and lack of diagnostic services and technical information at farm level. Our findings suggest that there is an urgent need for human capacity development for shrimp farm owners, workers and technicians. It is also important to improve access to quality inputs, rapid and affordable diagnostics and other technical services.

show abstract

Genomic and evolutionary features of two AHPND positive Vibrio parahaemolyticus strains isolated from shrimp (Penaeus monodon) of south-west Bangladesh

Cited by 18 publications

References 50 publications

Genetic diversity and toxic genes analysis of vibrio spp. isolated from white leg shrimp and marine fishes cultured in Tam G iang lagoon in Thua Thien Hue province, Vietnam

Genetic diversity and toxic genes analysis of vibrio spp. isolated from white leg shrimp and marine fishes cultured in Tam G iang lagoon in Thua Thien Hue province, Vietnam

Cloning and Expression of PirA gene of Vibrio parahaemolyticus Strain K5 Causing Acute Hepatopancreatic Necrosis Disease in Whiteleg Shrimp in E. coli Host Cell

Economic performance characterization of intensive shrimp (Penaeus monodon) farming systems in Bangladesh

Contact Info

Product

Resources

About