Genomic and biochemical characterization of antifungal compounds produced by Bacillus subtilis PMB102 against Alternaria brassicicola

Wu, Je-Jia; Chou, Hau-Ping; Huang, Jer-Shing; Deng, Wen-Ling

doi:10.1016/j.micres.2021.126815

Cited by 21 publications

(16 citation statements)

References 109 publications

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“…Compared to Bacillus vallismortis R2 and Bacillus megaterium L2, which secrete fengycins against A. alternata by eliciting swollen hyphae ( Li et al, 2015 ; Kaur et al, 2017 ), CEL showed a better effect regarding fungicidal activity. Wu et al also recently demonstrated that fengycin B produced by Bacillus subtilis PMB102 had antifungal activity against A. brassicae ABA-31 by activating swollen mycelia ( Wu et al, 2021 ). It is generally accepted that the action mode of lipopeptides relies on cell wall and membrane disruption, causing leakage of the cytoplasm and cell death ( Kaspar et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Genomic and Biocontrol Potential of the Crude Lipopeptide by Streptomyces bikiniensis HD-087 Against Magnaporthe oryzae

Liu

Wang

et al. 2022

Front. Microbiol.

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Rice blast caused by Magnaporthe oryzae is one of the most destructive plant diseases. The secondary metabolites of Streptomyces have potential as biological control agents against M. oryzae. However, no commercial secondary antimicrobial products of Streptomyces have been found by gene prediction, and, particularly relevant for this study, a biocontrol agent obtained from Streptomyces bikiniensis has yet to be found. In this research, genomic analysis was used to predict the secondary metabolites of Streptomyces, and the ability to develop biocontrol pharmaceuticals rapidly was demonstrated. The complete genome of the S. bikiniensis HD-087 strain was sequenced and revealed a number of key functional gene clusters that contribute to the biosynthesis of active secondary metabolites. The crude extract of lipopeptides (CEL) predicted by NRPS gene clusters was extracted from the fermentation liquid of S. bikiniensis HD-087 by acid precipitation followed by methanol extraction, and surfactins, iturins, and fengycins were identified by liquid chromatography-mass spectrometry (LC–MS). In vitro, the CEL of this strain inhibited spore germination and appressorial formation of M. oryzae by destroying membrane integrity and through the leakage of cellular components. In vivo, this CEL reduced the disease index of rice blast by approximately 76.9% on detached leaves, whereas its control effect on leaf blast during pot experiments was approximately 60%. Thus, the S. bikiniensis CEL appears to be a highly suitable alternative to synthetic chemical fungicides for controlling M. oryzae.

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Section: Discussionmentioning

confidence: 99%

Genomic and Biocontrol Potential of the Crude Lipopeptide by Streptomyces bikiniensis HD-087 Against Magnaporthe oryzae

Liu

Wang

et al. 2022

Front. Microbiol.

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“…Oxford cups were placed and respectively filled with 100 μL of two crude extracts, coating with 100 μg of sediment and methanol as a control, shown in Figure 5 a. The PDA plates were incubated at 28 °C for 5 days [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

“…are promising. A phyllosphere bacterium, B. subtilis PMB102 [ 18 ], isolated from the tomato leaf, was found to inhibit the growth of Alternaria brassicicola ABA-31 on PDA by 56.8% and suppress the Alternaria leaf spot on Chinese cabbage ( Brassica rapa ). Magnaporthe oryzae , which causes rice blast disease, can be controlled by using Bacillus safensis ; in field experiments, spraying with B. safensis B21 fermentation broth (29.48%) resulted in a significantly lower disease index compared with when carbendazim was used (37.88%) [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Bacillus amyloliquefaciens XJ-BV2007 on Growth of Alternaria alternata and Production of Tenuazonic Acid

Jia

Fan

Duan

et al. 2023

Toxins

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Large amounts of processing tomato are grown in Xinjiang, China. Tomato black spot disease, caused by Alternaria spp., and the produced alternaria toxins in tomato products are posing risks to human health. In this study, we isolated a rhizospheric bacterium, XJ-BV2007, from tomato (Solanum lycopersicum) fields, which we identified as Bacillus amyloliquefaciens. We found that this bacterium has a strong antagonistic effect against Alternaria alternata and reduces the accumulation of alternaria toxins in tomatoes. According to the antifungal activity of the bacteria-free filtrate, we revealed that B. amyloliquefaciens XJ-BV2007 suppresses A. alternata by the production of antifungal metabolites. Combining semi-preparative high-performance liquid chromatography, we employed UPLC-QTOF-MS analysis and the Oxford cup experiment to find that fengycin plays an important role in inhibiting A. alternata. This paper firstly reported that B. amyloliquefaciens efficiently controls tomato black spot disease and mycotoxins caused by A. alternata. B. amyloliquefaciens XJ-BV2007 may provide an alternative biocontrol strain for the prevention of tomato black spot disease.

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“…Bacillus species, especially its most known member bacillus subtilis , have long been known for their biocontrol properties, tackling diseases caused by fungal phytopathogens. Their antifungal activity has been attributed to a multitude of compounds including but not limited to lipopeptides (surfactins, iturins, fengycins), polyketides (bacillaene, macrolactin), enzymes, such as chitinases, and volatile compounds, such as pyrazine [ 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 , 125 , 126 , 127 , 128 , 129 ]. Although an increasing number of antifungal agents have been identified and purified from both bacillus and pseudomonas species, none have been able to make it through drug development for clinical adaptation.…”

Section: Natural Productsmentioning

confidence: 99%

Sources of Antifungal Drugs

Vanreppelen

Wuyts

Dijck

et al. 2023

JoF

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Due to their eukaryotic heritage, the differences between a fungal pathogen’s molecular makeup and its human host are small. Therefore, the discovery and subsequent development of novel antifungal drugs are extremely challenging. Nevertheless, since the 1940s, researchers have successfully uncovered potent candidates from natural or synthetic sources. Analogs and novel formulations of these drugs enhanced the pharmacological parameters and improved overall drug efficiency. These compounds ultimately became the founding members of novel drug classes and were successfully applied in clinical settings, offering valuable and efficient treatment of mycosis for decades. Currently, only five different antifungal drug classes exist, all characterized by a unique mode of action; these are polyenes, pyrimidine analogs, azoles, allylamines, and echinocandins. The latter, being the latest addition to the antifungal armamentarium, was introduced over two decades ago. As a result of this limited arsenal, antifungal resistance development has exponentially increased and, with it, a growing healthcare crisis. In this review, we discuss the original sources of antifungal compounds, either natural or synthetic. Additionally, we summarize the existing drug classes, potential novel candidates in the clinical pipeline, and emerging non-traditional treatment options.

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Genomic and biochemical characterization of antifungal compounds produced by Bacillus subtilis PMB102 against Alternaria brassicicola

Cited by 21 publications

References 109 publications

Genomic and Biocontrol Potential of the Crude Lipopeptide by Streptomyces bikiniensis HD-087 Against Magnaporthe oryzae

Genomic and Biocontrol Potential of the Crude Lipopeptide by Streptomyces bikiniensis HD-087 Against Magnaporthe oryzae

Effects of Bacillus amyloliquefaciens XJ-BV2007 on Growth of Alternaria alternata and Production of Tenuazonic Acid

Sources of Antifungal Drugs

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