Genomic and antigenic characterization of avipoxviruses

Schnitzlin, W.M.; Ghildyal, N. P.; Tripathy, Deoki N.

doi:10.1016/0168-1702(88)90058-5

Cited by 51 publications

(36 citation statements)

References 24 publications

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“…Because of the limited quantity of serum available from each bird, we could not verify our EIA results with another serologic test. Avipoxvirus species share a number of common antigens (Schnitzlein et al 1988). Antigens or pox virus DNA have been detected using immunofluorescent assay or PCR, respectively (Tripathy and Reed 1998).…”

Section: Methodsmentioning

confidence: 99%

RELATIVELY HIGH PREVALENCE OF POX-LIKE LESIONS IN HENSLOW'S SPARROW (AMMODRAMMUS HENSLOWII) AMONG NINE SPECIES OF MIGRATORY GRASSLAND PASSERINES IN WISCONSIN, USA

Ellison

Hofmeister

Ribic

et al. 2014

Journal of Wildlife Diseases

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ABSTRACT:Globally, Avipoxvirus species affect over 230 species of wild birds and can significantly impair survival. During banding of nine grassland songbird species (n5346 individuals) in southwestern Wisconsin, USA, we noted species with a 2-6% prevalence of poxlike lesions (possible evidence of current infection) and 4-10% missing digits (potential evidence of past infection). These prevalences approach those recorded among island endemic birds (4-9% and 9-20% for the Galapagos and Hawaii, respectively) for which Avipoxvirus species have been implicated as contributing to dramatic population declines. Henslow's Sparrow Ammodramus henslowii (n5165 individuals) had the highest prevalence of lesions (6.1%) and missing digits (9.7%). Among a subset of 26 Henslow's Sparrows from which blood samples were obtained, none had detectable antibody reactive to fowlpox virus antigen. However, four samples (18%) had antibody to canarypox virus antigen with test sample and negative control ratios (P/N values) ranging from 2.4 to 6.5 (median 4.3). Of four antibody-positive birds, two had lesions recorded (one was also missing a digit), one had digits missing, and one had no signs. Additionally, the birds with lesions or missing digits had higher P/N values than did the antibody-positive bird without missing digits or recorded lesions. This study represents an impetus for considering the impacts and dynamics of disease caused by Avipoxvirus among North American grassland bird species.

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Section: Methodsmentioning

confidence: 99%

RELATIVELY HIGH PREVALENCE OF POX-LIKE LESIONS IN HENSLOW'S SPARROW (AMMODRAMMUS HENSLOWII) AMONG NINE SPECIES OF MIGRATORY GRASSLAND PASSERINES IN WISCONSIN, USA

Ellison

Hofmeister

Ribic

et al. 2014

Journal of Wildlife Diseases

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“…Primary chicken embryo fibroblasts, chicken embryo kidney cells, chicken embryo dermis cells, or the permanent quail cell line QT-35, can also be used to propagate fowlpox virus (Ghildyal et al, 1989;Schnitzlein et al, 1988). The adaptation of virus strains to cell cultures is an important requirement for plaque formation, as not all strains will form plaques initially.…”

Section: Ii)mentioning

confidence: 99%

“…Restriction fragment length polymorphism (RFLP) analysis can be used for comparison of field isolates and vaccine strains of fowlpox virus (Ghildyal et al, 1989;Schnitzlein et al, 1988). However, this procedure is not used in routine diagnosis.…”

Section: Ii)mentioning

confidence: 99%

Isolation of Avipoxvirus from Tongue of Canaries (Serinus canaria) Show Severe Localized Proliferative Glossitis

et al. 2017

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“…3 However, even under these conditions, some antigenic heterogeneity among FPV vaccines and field isolates has been detected. 5 To possibly enhance the specificity of immunological tests and, more importantly, provide a means of differentiating between commercial FPV vaccines and naturally occurring viruses, we generated monoclonal antibodies (MAbs) against two distinct FPV proteins. 7 In the current study, the feasibility of using these 2 MAbs to identify and characterize FPV strains as well as to differentiate them from other avipoxviruses was evaluated.…”

mentioning

confidence: 99%

“…5 The FPV challenge strain was obtained from National Veterinary Science Laboratories. a For comparison, genetically and antigenically different avipox viruses were included in the study.…”

mentioning

confidence: 99%

Identification and Characterization of Fowlpox Virus Strains Using Monoclonal Antibodies

2003

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Abstract. The use of 2 monoclonal antibodies (MAbs), P1D9 and P2D4, which recognize different fowlpox virus (FPV) antigens, for the identification and characterization of FPV strains was evaluated. Initially, the MAbs were used in conjunction with a dot blot assay that enabled FPV to be differentiated from the avian herpesvirus, infectious laryngotracheitis virus. Confirmation of the specificity of these MAbs was provided by the demonstration that only FPV antigens were recognized by a combination of both antibodies when used for immunoblotting proteins contained in various avipoxviruses. Later, an antigenic characterization of 11 FPV field isolates, 6 FPV vaccine strains, and 3 pigeonpox virus vaccines was performed by Western blotting with the individual MAbs. Whereas MAb P2D4 consistently recognized a protein with an apparent molecular weight of 60 kD, there was variability in the size of the antigen that was immunoreactive with the other MAb. For example, MAb P1D9 recognized an antigen of apparent molecular weight of 46 kD in all vaccine strains except 2 of FPV origin. In these exceptions, either only a 39-kD or both a 42-and 46-kD protein were immunoreactive. As for the field isolates, a 39-kD antigen was recognized in 8 of them, whereas a 42-kD antigen was detected in the remaining 3. Therefore, the more extensive immunoblotting technique may facilitate FPV strain differentiation, whereas routine diagnosis of fowlpox could be accomplished by using the MAb-based dot blot assay.

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Genomic and antigenic characterization of avipoxviruses

Cited by 51 publications

References 24 publications

RELATIVELY HIGH PREVALENCE OF POX-LIKE LESIONS IN HENSLOW'S SPARROW (AMMODRAMMUS HENSLOWII) AMONG NINE SPECIES OF MIGRATORY GRASSLAND PASSERINES IN WISCONSIN, USA

RELATIVELY HIGH PREVALENCE OF POX-LIKE LESIONS IN HENSLOW'S SPARROW (AMMODRAMMUS HENSLOWII) AMONG NINE SPECIES OF MIGRATORY GRASSLAND PASSERINES IN WISCONSIN, USA

Isolation of Avipoxvirus from Tongue of Canaries (Serinus canaria) Show Severe Localized Proliferative Glossitis

Identification and Characterization of Fowlpox Virus Strains Using Monoclonal Antibodies

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