Genomic analysis provides insights into the Gordonia sp. W3S5 taxonomy and sulfur metabolism-related genes

Abstract

The Gordonia sp. W3S5, isolated from oil-polluted soil samples can remove sulfur from a variety of symmetric and asymmetric thiophenic compounds and diesel oil. Its draft genome sequence was comprised of 49 contigs, total genome size 4.86 Mb, and a G + C content of 67.50%. According to the current bacterial taxonomy procedures (16S rRNA gene sequence and overall genome-related index), the W3S5 was affiliated to Gordonia rubripertincta. Rapid Annotation using Subsystem Technology (ClassicRAST) server revealed that the W3S5 contains 4435 coding sequences, 404 subsystems and 60 sulfur metabolism genes. The RAST comparative genomic analysis showed that the genes connected with organic sulfur metabolism are majorly related to ssu and dszABC operons. Moreover, the comparison of orthologous gene clusters using OrthoVenn2 web server revealed a total of 4869 clusters, 2685 core orthologs, 632 shared orthologs and 112 unique ortholog clusters among the W3S5 and other type strains of Gordonia. This is the first report describing genome-based characterization of a Gordonia rubripertincta strain desulfurizing thiophenic compounds and diesel oil. The desulfurization potential of Gordonia rubripertincta W3S5 and genomic analyses revealed it as a valuable biocatalyst for process development to desulfurize a broad range of thiophenic sulfur-containing compounds, which are a major component of organic sulfur in petroleum oil.