Genomic analysis of the response of Arabidopsis thaliana to trinitrotoluene as revealed by cDNA microarrays

Mentewab, Ayalew; Cardoza, Vinitha; Stewart, C. Neal

doi:10.1016/j.plantsci.2004.10.021

Cited by 24 publications

(14 citation statements)

References 55 publications

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“…It is generally thought that antioxidation activity related to class III peroxidases could contribute in part to metal tolerance (Chiang et al, 2006). Trinitrotoluene (TNT) treatment that also results in oxidative stress induced one specific gene in common with aluminium treatment and another one in common with arsenic treatment (Mentewab et al, 2005), possibly indicating a role for these peroxidases in antioxidation activity.…”

Section: Stresses Defence and Tolerancementioning

confidence: 99%

Specific functions of individual class III peroxidase genes

Cosio

Dunand

2009

Journal of Experimental Botany

361

288

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In higher plants, class III peroxidases exist as large multigene families (e.g. 73 genes in Arabidopsis thaliana). The diversity of processes catalysed by peroxidases as well as the large number of their genes suggests the possibility of a functional specialization of each isoform. In addition, the fact that peroxidase promoter sequences are very divergent and that protein sequences contain both highly conserved domains and variable regions supports this hypothesis. However, two difficulties are associated with the study of the function of specific peroxidase genes: (i) the modification of the expression of a single peroxidase gene often results in no visible mutant phenotype, because it is compensated by redundant genes; and (ii) peroxidases show low substrate specificity in vitro resulting in an unreliable indication of peroxidase specific activity unless complementary data are available. The generalization of molecular biology approaches such as whole transcriptome analysis and recombinant DNA combined with biochemical approaches provide unprecedented tools for overcoming these difficulties. This review highlights progress made with these new techniques for identifying the specific function of individual class III peroxidase genes taking as an example the model plant A. thaliana, as well as discussing some other plants.

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Section: Stresses Defence and Tolerancementioning

confidence: 99%

Specific functions of individual class III peroxidase genes

Cosio

Dunand

2009

Journal of Experimental Botany

361

288

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“…Their application has immense potential for phytoremediation research, given our scarce knowledge of relevant cellular processes (see above). While these tools have been used mainly to investigate metal hyperaccumulation (reviewed recently by Hooda, 2007), less studies have focused on the molecular changes occurring in the presence of xenobiotics such as herbicides (Baerson et al, 2005;Castro et al, 2005;Mezzari et al, 2005;Zhang et al, 2007) or explosives (Ekman et al, 2003(Ekman et al, , 2005Patel et al, 2004;Mentewab et al, 2005). By using a proteomic approach, several poplar proteins putatively involved in the degradation of PCBs have been recently identified (Fig.…”

Section: «Omics» Approachesmentioning

confidence: 99%

Phytoremediation of organic pollutants: a review

Campos

Merino

Casado

et al. 2008

Span. j. agric. res.

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Many endeavours associated with human progress and welfare have the undesirable side-effect of spreading vast amounts of hazardous compounds and heavy metals into the environment. These pollutants enter the food web relentlessly and pose a serious threat to human health and the integrity of ecosystems worldwide. The cleanup of polluted substrates is carried out mainly through energy-intensive engineering processes (Salvato et al., 2003). These require sophisticated and costly equipment but often give unsatisfactory results, such as incomplete pollutant removal, emission of greenhouse gases, the destruction of soil structure or severe landscape alteration. For many pollutants, feasible and cost-effective technologies have yet to be developed. Review. Phytoremediation of organic pollutants AbstractPhytoremediation consists of a set of innovative technologies for environmental cleanup that takes advantage of the unique extractive and metabolic capabilities of plants. This technology presents clear benefits over traditional methods, including wide applicability, ecological value and cost-effectiveness. Whereas organic pollutants can be degraded to less toxic forms by plants, or even mineralized, most research has focused so far on heavy metals, which are immutable. We analyze here the possible causes of this disparity and present an overview of current knowledge on the mechanisms used by plants to detoxify relevant organic pollutants. The impact of recent advances in molecular technology and the prospects of using transgenic plants are discussed.Additional key words: organic pollutants, phytotechnology, plant genetic engineering, xenobiotics. Resumen Revisión. Fitorremediación de contaminantes orgánicosLa fitorremediación comprende un conjunto de tecnologías innovadoras de descontaminación ambiental, que trata de explotar la extraordinaria capacidad extractiva y metabólica de las plantas. Esta tecnología ofrece numerosas ventajas frente a los métodos tradicionales de descontaminación, incluida su amplia aplicabilidad y claros beneficios ecológicos y económicos. Aunque las plantas pueden transformar los contaminantes orgánicos en moléculas menos tóxicas, o incluso degradarlos por completo, las investigaciones en este campo se han centrado hasta ahora en los metales pesados. En esta revisión se analizan brevemente las causas y se presenta una visión panorámica de los mecanismos que permiten a las plantas degradar contaminantes orgánicos relevantes. También se discute brevemente el impacto que están teniendo las nuevas tecnologías del ámbito molecular y las posibilidades que ofrece el uso de plantas transgénicas.Palabras clave adicionales: contaminantes orgánicos, fitotecnología, ingeniería genética de plantas, xenobióticos.

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“…To overcome these challenges, the Army needs improved technological approaches to the assessment of the environmental effects of contaminants [5]. The di-versity of wildlife and the large number of threatened and endangered species inhabiting these 25 million acres are a unique resource for the nation.…”

Section: Introductionmentioning

confidence: 99%

“…Plants are also used as tools in bioremediation (phytoremediation) due to their ability to take up metals or other contaminants [4,[10][11][12][13]. Currently, genetically modified Arabidopsis plants are being designed to indicate the presence of explosives in areas used for military training through the expression of genes for color changes in the plants [5]. Currently, genetically modified Arabidopsis plants are being designed to indicate the presence of explosives in areas used for military training through the expression of genes for color changes in the plants [5].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, land use needs for activities such as training exercises, munitions production, and weapon systems testing are constrained by the challenge of maintaining environmentally sound ecosystems, preserving biodiversity, and managing natural resources effectively and sensibly. To overcome these challenges, the Army needs improved technological approaches to the assessment of the environmental effects of contaminants [5].…”

Section: Introductionmentioning

confidence: 99%

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DNA microarrays detect effects of soil contamination on Arabidopsis thaliana gene expression

Magrini

Basu

Spotila

et al. 2008

Enviro Toxic and Chemistry

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Soil contamination, such as heavy metals and benzene compounds, is a widespread problem on military installations. It is important to be able to determine the effects of soil contamination before any adverse effects appear in organisms in surrounding areas. We examined gene expression in Arabidopsis thaliana grown in soil from three sites at the Radford Army Ammunition Plant in Radford, Virginia, USA, using DNA microarrays. We analyzed soil, germination, and growth rate to compare with the microarray data. Soil contamination affected both external phenotype and gene expression. Plants grown in soil with high levels of contaminants were chloritic and were smaller than control plants grown in potting soil. Plants grown in soil with the highest copper concentration had the lowest growth rates and had genes up-regulated across several functional groups. Plants grown in soils with elevated lead had many genes down-regulated that were related to photosystem II, metabolism, cellular transport, and protein synthesis. Genes consistently up-regulated across most microarrays were genes related to photosystem I, genes related to water deprivation and oxidative stress response, heat shock proteins, and toxin catabolism genes such as glutathiones. DNA microarrays, in concert with a model genetic organism such as A. thaliana, were an effective assessment tool to determine the presence of toxic substances in soil at a site used for the production of military explosives.

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Genomic analysis of the response of Arabidopsis thaliana to trinitrotoluene as revealed by cDNA microarrays

Cited by 24 publications

References 55 publications

Specific functions of individual class III peroxidase genes

Specific functions of individual class III peroxidase genes

Phytoremediation of organic pollutants: a review

DNA microarrays detect effects of soil contamination on Arabidopsis thaliana gene expression

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