Genomic analysis of parallel-evolved cyanobacterium Synechocystis sp. PCC 6803 under acid stress

Uchiyama, Junji; Kanesaki, Yu; Iwata, Naoya; Asakura, Ryousuke; Funamizu, Kento; Tasaki, Rizumu; Agatsuma, Mina; Tahara, Hiroko; Matsuhashi, Ayumi; Yoshikawa, Hideki; Ogawa, Satoshi; Ohta, Hisataka

doi:10.1007/s11120-015-0111-3

Cited by 29 publications

(12 citation statements)

References 52 publications

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“…with an improved growth performance under acid stress at pH 5.5. A subsequent whole-genome sequence analysis suggested that SNPs in certain genes are involved in acid stress tolerance [ 136 ]. Similarity, heat-tolerant strains of Synechocystis sp.…”

Section: Adaptive Laboratory Evolutionmentioning

confidence: 99%

Microalgae for the production of lipid and carotenoids: a review with focus on stress regulation and adaptation

Sun

Ren

Zhao

et al. 2018

Biotechnol Biofuels

318

129

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Microalgae have drawn great attention as promising sustainable source of lipids and carotenoids. Their lipid and carotenoids accumulation machinery can be trigged by the stress conditions such as nutrient limitation or exposure to the damaging physical factors. However, stressful conditions often adversely affect microalgal growth and cause oxidative damage to the cells, which can eventually reduce the yield of the desired products. To overcome these limitations, two-stage cultivation strategies and supplementation of growth-promoting agents have traditionally been utilized, but developing new highly adapted strains is theoretically the simplest strategy. In addition to genetic engineering, adaptive laboratory evolution (ALE) is frequently used to develop beneficial phenotypes in industrial microorganisms during long-term selection under specific stress conditions. In recent years, many studies have gradually introduced ALE as a powerful tool to improve the biological properties of microalgae, especially for improving the production of lipid and carotenoids. In this review, strategies for the manipulation of stress in microalgal lipids and carotenoids production are summarized and discussed. Furthermore, this review summarizes the overall state of ALE technology, including available selection pressures, methods, and their applications in microalgae for the improved production of lipids and carotenoids.

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Section: Adaptive Laboratory Evolutionmentioning

confidence: 99%

Microalgae for the production of lipid and carotenoids: a review with focus on stress regulation and adaptation

Sun

Ren

Zhao

et al. 2018

Biotechnol Biofuels

318

129

View full text Add to dashboard Cite

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“…Recent genomic analysis of stress-evolved Synechocystis sp. PCC 6803 strains also revealed interesting information in adaptive evolution and stress response under high temperature or low pH [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of Specific Variations in a Non-Motile Strain of Cyanobacterium Synechocystis sp. PCC 6803 Originated from ATCC 27184 by Whole Genome Resequencing

Ding

Wei

2015

IJMS

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Cyanobacterium Synechocystis sp. PCC 6803 is a widely used model organism in basic research and biofuel biotechnology application. Here, we report the genomic sequence of chromosome and seven plasmids of a glucose-tolerant, non-motile strain originated from ATCC 27184, GT-G, in use at Guangzhou. Through high-throughput genome re-sequencing and verification by Sanger sequencing, eight novel variants were identified in its chromosome and plasmids. The eight novel variants, especially the five non-silent mutations might have interesting effects on the phenotype of GT-G strains, for example the truncated Sll1895 and Slr0322 protein. These resequencing data provide background information for further research and application based on the GT-G strain and also provide evidence to study the evolution and divergence of Synechocystis 6803 globally.

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“…However, overall internal pH (the average of cytosol and thylakoid pH) across the same external pH range was only somewhat affected, suggesting that the observed limitation of energy supply might not be due to ΔpH alone. In support of the hypothesis that ATP supply might limit growth as pH is reduced, two strains of Synechocystis 6803 cells acclimated to pH 5.5 growth over 3 months independently acquired mutations in genes encoding F 1 –F 0 ATP synthase components ( Uchiyama et al, 2015 ), although these mutations are, as yet, functionally uncharacterized. Furthermore, experimental investigation of this hypothesis would require greater investigation of internal pH changes within the cell during photosynthesis and respiration, which to date has proven difficult ( Berry et al, 2005 ).…”

Section: Proposed Effects Of Environmental Ph On Ps II Mutantsmentioning

confidence: 99%

Environmental pH and the Requirement for the Extrinsic Proteins of Photosystem II in the Function of Cyanobacterial Photosynthesis

2016

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In one of the final stages of cyanobacterial Photosystem II (PS II) assembly, binding of up to four extrinsic proteins to PS II stabilizes the oxygen-evolving complex (OEC). Growth of cyanobacterial mutants deficient in certain combinations of these thylakoid-lumen-associated polypeptides is sensitive to changes in environmental pH, despite the physical separation of the membrane-embedded PS II complex from the external environment. In this perspective we discuss the effect of environmental pH on OEC function and photoautotrophic growth in cyanobacteria with reference to pH-sensitive PS II mutants lacking extrinsic proteins. We consider the possibilities that, compared to pH 10.0, pH 7.5 increases susceptibility to PS II-generated reactive oxygen species (ROS) causing photoinhibition and reducing PS II assembly in some mutants, and that perturbations to channels in the lumenal regions of PS II might alter the accessibility of water to the active site as well as egress of oxygen and protons to the thylakoid lumen. Reduced levels of PS II in these mutants, and reduced OEC activity arising from the disruption of substrate/product channels, could reduce the trans-thylakoid pH gradient (ΔpH), leading to the impairment of photosynthesis. Growth of some PS II mutants at pH 7.5 can be rescued by elevating CO2 levels, suggesting that the pH-sensitive phenotype might primarily be an indirect result of back-pressure in the electron transport chain that results in heightened production of ROS by the impaired photosystem.

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Genomic analysis of parallel-evolved cyanobacterium Synechocystis sp. PCC 6803 under acid stress

Cited by 29 publications

References 52 publications

Microalgae for the production of lipid and carotenoids: a review with focus on stress regulation and adaptation

Microalgae for the production of lipid and carotenoids: a review with focus on stress regulation and adaptation

Identification of Specific Variations in a Non-Motile Strain of Cyanobacterium Synechocystis sp. PCC 6803 Originated from ATCC 27184 by Whole Genome Resequencing

Environmental pH and the Requirement for the Extrinsic Proteins of Photosystem II in the Function of Cyanobacterial Photosynthesis

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