Genomic analysis of multidrug-resistant Escherichia coli strains carrying the mcr-1 gene recovered from pigs in Lima-Peru

E., Luis Luna; Huamán, Dennis Carhuaricra; Cueva, Carmen Rodriguez; Gonzales, Carla Durán; León, Yennifer Ignación; Espejo, Thalia Silvestre; Monge, Geraldine Marcelo; A., Raúl Rosadio; Maturrano, Lenin

doi:10.1016/j.cimid.2023.102019

Cited by 5 publications

(7 citation statements)

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“…The growing demand for animal products has led to increased animal farming practices, leading to a number of consequences, including defective processing practices and a high risk of contamination by foodborne pathogens [ 3 , 4 ]. Pigs are one of the most consumed livestock in the world, contributing about 33% of the total global meat production [ 5 , 6 ]. As part of management measures to promote weight gain in pigs, antibiotics are used as growth enhancers to prevent the colonization of pathogenic bacteria and the uncontrolled growth of intestinal microorganisms [ 5 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…Pigs are one of the most consumed livestock in the world, contributing about 33% of the total global meat production [ 5 , 6 ]. As part of management measures to promote weight gain in pigs, antibiotics are used as growth enhancers to prevent the colonization of pathogenic bacteria and the uncontrolled growth of intestinal microorganisms [ 5 , 7 ]. Food-producing animals are considered important reservoirs of foodborne pathogens [ 3 ], especially antibiotic-resistant E. coli strains.…”

Section: Introductionmentioning

confidence: 99%

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Unveiling Antibiotic Resistance, Clonal Diversity, and Biofilm Formation in E. coli Isolated from Healthy Swine in Portugal

Silva,

Dapkevicius

et al. 2024

Pathogens

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Escherichia coli, a commensal microorganism found in the gastrointestinal tract of human and animal hosts, plays a central role in agriculture and public health. Global demand for animal products has promoted increased pig farming, leading to growing concerns about the prevalence of antibiotic-resistant E. coli strains in swine populations. It should be noted that a significant portion of antibiotics deployed in swine management belong to the critically important antibiotics (CIA) class, which should be reserved for human therapeutic applications. This study aimed to characterize the prevalence of antibiotic resistance, genetic diversity, virulence characteristics, and biofilm formation of E. coli strains in healthy pigs from various farms across central Portugal. Our study revealed high levels of antibiotic resistance, with resistance to tetracycline, ampicillin, tobramycin, and trimethoprim-sulfamethoxazole. Multidrug resistance is widespread, with some strains resistant to seven different antibiotics. The ampC gene, responsible for broad-spectrum resistance to cephalosporins and ampicillin, was widespread, as were genes associated with resistance to sulfonamide and beta-lactam antibiotics. The presence of high-risk clones, such as ST10, ST101, and ST48, are a concern due to their increased virulence and multidrug resistance profiles. Regarding biofilm formation, it was observed that biofilm-forming capacity varied significantly across different compartments within pig farming environments. In conclusion, our study highlights the urgent need for surveillance and implementation of antibiotic management measures in the swine sector. These measures are essential to protect public health, ensure animal welfare, and support the swine industry in the face of the growing global demand for animal products.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Unveiling Antibiotic Resistance, Clonal Diversity, and Biofilm Formation in E. coli Isolated from Healthy Swine in Portugal

Silva,

Dapkevicius

et al. 2024

Pathogens

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show abstract

“…The growing demand for animal products has resulted in increased animal farming, leading to a number of consequences, including defective processing practices, and a high risk of contamination by foodborne pathogens (Heredia and García 2018; Silva et al 2023). Pigs are one of the most consumed livestock in the world, contributing to about 33% of the total global meat production (Michele 2023;Espinoza et al 2023). Food-producing animals are considered important reservoirs of foodborne pathogens (Heredia and García 2018), especially antibiotic-resistant E. coli strains.…”

Section: Introductionmentioning

confidence: 99%

“…Our study revealed a complex landscape of antimicrobial resistance genes among the 46 E. coli isolates, with the most prominent gene, ampC, present in 86.95% of the isolates, but determinants of sulfonamide (sul2, sul3), tetracycline (tetA, tetB), aminoglycoside (aac(6)-Ib, aph(3), acc(3)-II, acc(3)-IV), chloramphenicol (cmlA), beta-lactam (blaTEM, blaIMP), and quinolone (parC, qnrS) resistance were also found. A study in Peru (Espinoza et al 2023) also identi ed aminoglycoside, uoroquinolone (qnrB1, and qnrS1 genes), tetracycline (tetA, tetB, and tetM), chloramphenicol (cmlA1), and sulfonamide resistance determinants (sul2 and sul3). The prevalence of sul2, sul3, and cmlA1 in isolates from pig farming suggests that resistance to these latter antibiotics is common in swine populations (Dawangpa et Bio lm formation in E coli is a survival strategy, granting resistance to antibiotics and disinfectants, but poses challenges for eradication and control, especially with the increasing prevalence of MDR strains (Lajhar et al 2018;Milton et al 2023).…”

mentioning

confidence: 99%

“…al. 2022;Espinoza et al 2023). A study on Thai swine farms(Dawangpa et al 2022) revealed that isolates from piglet feces had the highest numbers of antimicrobial resistance genes, and identi ed eight antimicrobial resistance determinants (blaTEM, tetA, drfA12, sul3, cmlA, aaadA1, Sul2, and catB), linked to trimethoprim, sulfonamide, aminoglycoside, and tetracycline resistance among these isolates(Dawangpa et al 2022).…”

mentioning

confidence: 99%

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Unveiling antibiotic resistance, clonal diversity, and biofilm formation in E. coli for safer pork production

Silva,

Dapkevicius,

Azevedo

et al. 2023

Preprint

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Escherichia coli, a commensal microorganism found in the gastrointestinal tract of human and animal hosts, plays a central role in agriculture and public health. Global demand for animal products has promoted an increase in pig farming, leading to growing concerns about the prevalence of antibiotic-resistant E. coli strains in swine populations. It should be noted that a significant portion of antibiotics deployed in swine management belong to the critically important antibiotics (CIA) class, which should be reserved for human therapeutic applications. This study aimed at characterizing the prevalence of antibiotic resistance, genetic diversity, and virulence characteristics of E. coli strains isolated in different pig farms in Portugal. Our study revealed high levels of antibiotic resistance, with resistance to tetracycline, ampicillin, tobramycin, and trimethoprim-sulfamethoxazole. Multidrug resistance is widespread, with some strains showing resistance to seven different antibiotics. The ampC gene, responsible for broad-spectrum resistance to cephalosporins and ampicillin, was widespread, as were genes associated with resistance to sulfonamide and beta-lactam antibiotics. The presence of high-risk clones, such as ST10, ST101 and ST48 are a concern due to their increased virulence and multidrug resistance. Regarding biofilm formation, it was observed that biofilm-forming capacity varied significantly across different compartments within pig farming environments. In conclusion, our study highlights the urgent need for surveillance and implementation of antibiotic management measures in the swine sector. These measures are essential to protect public health, ensure animal welfare and support the swine industry in the face of the growing global demand for animal products.

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Detecting Class 1 Integrons and Their Variable Regions in Escherichia coli Whole-Genome Sequences Reported from Andean Community Countries

Solis,

Loaiza,

Torres-Elizalde

et al. 2024

Antibiotics

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Various genetic elements, including integrons, are known to contribute to the development of antimicrobial resistance. Class 1 integrons have been identified in E. coli isolates and are associated with multidrug resistance in countries of the Andean Community. However, detailed information on the gene cassettes located on the variable regions of integrons is lacking. Here, we investigated the presence and diversity of class 1 integrons, using an in silico approach, in 2533 whole-genome sequences obtained from EnteroBase. IntFinder v1.0 revealed that almost one-third of isolates contained these platforms. Integron-bearing isolates were associated with environmental, food, human, and animal origins reported from all countries under scrutiny. Moreover, they were identified in clones known for their pathogenicity or multidrug resistance. Integrons carried cassettes associated with aminoglycoside (aadA), trimethoprim (dfrA), cephalosporin (blaOXA; blaDHA), and fluoroquinolone (aac(6′)-Ib-cr; qnrB) resistance. These platforms showed higher diversity and larger numbers than previously reported. Moreover, integrons carrying more than three cassettes in their variable regions were determined. Monitoring the prevalence and diversity of genetic elements is necessary for recognizing emergent patterns of resistance in pathogenic bacteria, especially in countries where various factors are recognized to favor the selection of resistant microorganisms.

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Genomic analysis of multidrug-resistant Escherichia coli strains carrying the mcr-1 gene recovered from pigs in Lima-Peru

Cited by 5 publications

References 76 publications

Unveiling Antibiotic Resistance, Clonal Diversity, and Biofilm Formation in E. coli Isolated from Healthy Swine in Portugal

Unveiling Antibiotic Resistance, Clonal Diversity, and Biofilm Formation in E. coli Isolated from Healthy Swine in Portugal

Unveiling antibiotic resistance, clonal diversity, and biofilm formation in E. coli for safer pork production

Detecting Class 1 Integrons and Their Variable Regions in Escherichia coli Whole-Genome Sequences Reported from Andean Community Countries

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