Genomic Analysis of<i>Blomia tropicalis</i>Identifies Novel Allergens for Component-Resolved Diagnosis of Mite Allergy

Xiong, Qing; Liu, Xiaoyu; Wan, Angel Tsz-Yau; Malainual, Nat; Xiao, Xiaojun; Tang, Man-Fung; Ng, Judy Kin-Wing; Shin, Soo-Kyung; Sio, Yang Yie; WANG, MINGQIANG; Sun, Baoqing; Leung, Ting‐Fan; Chew, Fook Tim; Tungtrongchitr, Anchalee; Tsui, Stephen Kwok-Wing

doi:10.1101/2023.02.09.527948

Cited by 3 publications

(1 citation statement)

References 45 publications

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“…New mite allergen groups are constantly being identified worldwide [9,11,14,15]. So far, there are 33 different groups acknowledged for Dermatophagoides pteronyssinus allergens [9,11].…”

Section: Introductionmentioning

confidence: 99%

Removal of N-Terminal Peptide Impacts Structural Aspects of an IgE-Reactive Recombinant Der p 5

Vieira,

Silva,

Silveira

et al. 2023

Allergies

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(1) Background: Modification of the structural elements of allergens is widely used in the field of allergies. The goal of the present research was to express, purify, and characterize the shortened recombinant group 5 allergen of Dermatophagoides pteronyssinus (rDer p 5). (2) Methods: rDer p 5 storage stability and aggregation capacity were explored through in silico analysis, dynamic light scattering (DLS), and SDS-PAGE. Serum IgE reactivity and cytokine amount were investigated in sera or cell culture supernatants through ELISA, MULTIPLEX®, and Western blot analysis using sera from sensitized humans from Brazil, Colombia, and Ecuador. (3) Results: Dimeric rDer p 5 was detected through native PAGE, and this result was confirmed by data from DLS. The protein was thermically stable, as it did not degrade at 4 °C for 21 days. The shortened rDer p 5 was classified as a major IgE allergen in Brazil and Colombia, but minor in Ecuador. IL-13, IL-10, IL-1β, and IL-6 were significantly elevated in the sera of rDer p 5-reactive patients. The same cytokines plus IL-5 were more secreted by human cells upon rDer p 5 stimulation. (4) Conclusions: N-terminal peptide deletion led to a higher rDer p 5 folding stability, which, even though dimeric, was an IgE-reactive protein. Therefore, rDer p 5 could be used for molecular diagnostic applications or as backbone for hypoallergen design.

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“…New mite allergen groups are constantly being identified worldwide [9,11,14,15]. So far, there are 33 different groups acknowledged for Dermatophagoides pteronyssinus allergens [9,11].…”

Section: Introductionmentioning

confidence: 99%

Removal of N-Terminal Peptide Impacts Structural Aspects of an IgE-Reactive Recombinant Der p 5

Vieira,

Silva,

Silveira

et al. 2023

Allergies

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Predicting Blomia tropicalis allergens using a multiomics approach

Hubert,

Vrtala,

Sopko

et al. 2023

Clinical & Translational All

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BackgroundThe domestic mite Blomia tropicalis is a major source of allergens in tropical and subtropical regions. Despite its great medical importance, the allergome of this mite has not been sufficiently studied. Only 14 allergen groups have been identified in B. tropicalis thus far, even though early radioimmunoelectrophoresis techniques (27 uncharacterized allergen complexes) and comparative data based on 40 allergen groups officially recognized by the World Health Organization (WHO)/IUIS in domestic astigmatid mites suggest the presence of a large set of additional allergens.MethodsHere, we employ a multiomics approach to assess the allergome of B. tropicalis using genomic and transcriptomic sequence data and perform highly sensitive protein abundance quantification.FindingsAmong the 14 known allergen groups, we confirmed 13 (one WHO/IUIS allergen, Blo t 19, was not found) and identified 16 potentially novel allergens based on sequence similarity. These data indicate that B. tropicalis shares 27 known/deduced allergen groups with pyroglyphid house dust mites (genus Dermatophagoides). Among these groups, five allergen‐encoding genes are highly expressed at the transcript level: Blo t 1, Blo t 5, Blo t 21 (known), Blo t 15, and Blo t 18 (predicted). However, at the protein level, a different set of most abundant allergens was found: Blo t 2, 10, 11, 20 and 21 (mite bodies) or Blo t 3, 4, 6 and predicted Blo t 13, 14 and 36 (mite feces).InterpretationWe report the use of an integrated omics method to identify and predict an array of mite allergens and advanced, label‐free proteomics to determine allergen protein abundance. Our research identifies a large set of novel putative allergens and shows that the expression levels of allergen‐encoding genes may not be strictly correlated with the actual allergenic protein abundance in mite bodies.

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PredictingBlomia tropicalisallergens using a multiomics approach

Hubert,

Vrtala,

Sopko

et al. 2023

Preprint

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The domestic mite Blomia tropicalis is a major source of allergens in tropical and subtropical regions. Despite its great medical importance, the allergome of this mite is not sufficiently studied. Only 14 allergen groups have been identified in B. tropicalis so far, even though early radioimmunoelectrophoresis techniques (27 uncharacterized allergen complexes) and comparative data based on 40 allergen groups officially recognized by WHO/IUIS in domestic astigmatid mites, suggest that a large set of additional allergens may be present. Here, we use a multiomics approach to assess the allergome of B. tropicalis using genomic, transcriptomic sequence data, and a highly sensitive protein abundance quantification. Out of 14 known allergen groups, we confirmed 13 (one WHO/IUIS allergen, Blo t 19, was found to be a contaminant originated from a nematode) and identified 16 potentially novel allergens using sequence similarity. These data indicate that B. tropicalis shares 27 known/deduced allergen groups with pyroglyphid house dust mites (genus Dermatophagoides). Among them, five allergen-encoding genes were highly expressed at the transcript level: Blo t 1, Blo t 5, Blo t 21 (known), Blo t 15, and Blo t 18 (predicted). However, at the protein level, a different set of most abundant allergens was found: Blo t 2, 10, 11, 20 and 21 (mite bodies) or Blo t 3, 4, 6 and predicted Blo t 13, 14 and 36 (mite feces). We show the use of an integrated omics method to identify and predict an array of mite allergens and advanced, label-free proteomics to determine allergen protein abundance. Our research identifies a large set of novel putative allergens and shows that expression levels of allergen-encoding genes may not be strictly correlated with the actual allergenic protein abundance in the mite bodies.

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Genomic Analysis ofBlomia tropicalisIdentifies Novel Allergens for Component-Resolved Diagnosis of Mite Allergy

Cited by 3 publications

References 45 publications

Removal of N-Terminal Peptide Impacts Structural Aspects of an IgE-Reactive Recombinant Der p 5

Removal of N-Terminal Peptide Impacts Structural Aspects of an IgE-Reactive Recombinant Der p 5

Predicting Blomia tropicalis allergens using a multiomics approach

PredictingBlomia tropicalisallergens using a multiomics approach

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