Genomic analysis of detoxification genes in the mosquito Aedes aegypti

Strode, Clare; Wondji, Charles S.; David, Jean-Philippe; Hawkes, Nicola J.; Lumjuan, Nongkran; Nelson, David R.; Drane, David R.; Karunaratne, S.H.P.P.; Hemingway, Janet; Black, William C.; Ranson, Hilary

doi:10.1016/j.ibmb.2007.09.007

Cited by 310 publications

(328 citation statements)

References 41 publications

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“…Afterward, the development of mosquito DNA microarrays allowed screening for resistance genes based on their differential transcription, leading to the identification of detoxification genes conferring resistance (David et al 2005Strode et al 2008;Vontas et al 2010;Edi et al 2014). However, these screenings focused on differential transcription while genomic changes such as CNVs and polymorphisms were neglected.…”

Section: Discussionmentioning

confidence: 99%

“…Most were previously found significantly overtranscribed in pyrethroid resistant Ae. aegypti populations according to data publicly available in the VectorBase expression browser (https://vectorbase.org, data sets from Strode et al 2008;Marcombe et al 2009;Poupardin et al 2012;David et al 2014). The majority of CNVs observed between resistant and susceptible populations were <2.5-fold, suggesting a predominance of duplications.…”

Section: Gene Amplifications Associated With Pyrethroid Resistancementioning

confidence: 99%

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Identifying genomic changes associated with insecticide resistance in the dengue mosquito Aedes aegypti by deep targeted sequencing

et al. 2015

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The capacity of mosquitoes to resist insecticides threatens the control of diseases such as dengue and malaria. Until alternative control tools are implemented, characterizing resistance mechanisms is crucial for managing resistance in natural populations. Insecticide biodegradation by detoxification enzymes is a common resistance mechanism; however, the genomic changes underlying this mechanism have rarely been identified, precluding individual resistance genotyping. In particular, the role of copy number variations (CNVs) and polymorphisms of detoxification enzymes have never been investigated at the genome level, although they can represent robust markers of metabolic resistance. In this context, we combined target enrichment with high-throughput sequencing for conducting the first comprehensive screening of gene amplifications and polymorphisms associated with insecticide resistance in mosquitoes. More than 760 candidate genes were captured and deep sequenced in several populations of the dengue mosquito Ae. aegypti displaying distinct genetic backgrounds and contrasted resistance levels to the insecticide deltamethrin. CNV analysis identified 41 gene amplifications associated with resistance, most affecting cytochrome P450s overtranscribed in resistant populations. Polymorphism analysis detected more than 30,000 variants and strong selection footprints in specific genomic regions. Combining Bayesian and allele frequency filtering approaches identified 55 nonsynonymous variants strongly associated with resistance. Both CNVs and polymorphisms were conserved within regions but differed across continents, confirming that genomic changes underlying metabolic resistance to insecticides are not universal. By identifying novel DNA markers of insecticide resistance, this study opens the way for tracking down metabolic changes developed by mosquitoes to resist insecticides within and among populations.

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Section: Discussionmentioning

confidence: 99%

Section: Gene Amplifications Associated With Pyrethroid Resistancementioning

confidence: 99%

Identifying genomic changes associated with insecticide resistance in the dengue mosquito Aedes aegypti by deep targeted sequencing

et al. 2015

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“…aegypti is not an isolated phenomenon; it has already been reported in latin America (Rodríguez et al 2002(Rodríguez et al , 2007 and the French West Indies (Marcombe et al 2009a). Studies from these regions demonstrated that multiple insecticide resistance may be due to the combination the overexpression of genes -such as mixed-function oxidases -involved in metabolic resistance (Flores et al 2006, Strode et al 2008, Marcombe et al 2009b) and target site mutations on the sodium channel gene. Such mutations are extensively described in insects and are known to confer resistance to pyrethroids and DDT called knockdown resistance (kdr).…”

Section: Table IImentioning

confidence: 96%

Multiple insecticide resistance in Aedes aegypti (Diptera: Culicidae) populations compromises the effectiveness of dengue vector control in French Guiana

Dusfour

Thalmensy

Gaborit

et al. 2011

Mem. Inst. Oswaldo Cruz

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In French Guiana, pyrethroids and organophosphates have been used for many years against Aedes aegypti. We aimed to establish both the resistance level of Ae. aegypti and the ultra low volume spray efficacy to provide mosquito control services with practical information to implement vector control and resistance management. Resistance to deltamethrin and fenitrothion was observed. In addition, the profound loss of efficacy of AquaK'othrine® and the moderate loss of efficacy of Paluthion® 500 were recorded. Fenitrothion remained the most effective candidate for spatial application in French Guiana until its removal in December 2010. Further investigation of the mechanism of resistance to deltamethrin demonstrated the involvement of mixed-function oxidases and, to a lesser extent, of carboxylesterases. However, these observations alone cannot explain the level of insecticide resistance we observed during tube and cage tests

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“…Genome sequencing projects have identified a large number of P450 sequences in eukaryotes (1)(2)(3)(4)(5). Originally identified as monooxygenases, P450 enzymes are now known to catalyze an extremely diverse range of chemical reactions important both in developmental processes and in the detoxification of foreign compounds (6)(7)(8).…”

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confidence: 99%

Characterization of Drosophila melanogaster cytochrome P450 genes

Chung

Sztal

Pasricha

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

255

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Cytochrome P450s form a large and diverse family of hemecontaining proteins capable of carrying out many different enzymatic reactions. In both mammals and plants, some P450s are known to carry out reactions essential for processes such as hormone synthesis, while other P450s are involved in the detoxification of environmental compounds. In general, functions of insect P450s are less well understood. We characterized Drosophila melanogaster P450 expression patterns in embryos and 2 stages of third instar larvae. We identified numerous P450s expressed in the fat body, Malpighian (renal) tubules, and in distinct regions of the midgut, consistent with hypothesized roles in detoxification processes, and other P450s expressed in organs such as the gonads, corpora allata, oenocytes, hindgut, and brain. Combining expression pattern data with an RNA interference lethality screen of individual P450s, we identify candidate P450s essential for developmental processes and distinguish them from P450s with potential functions in detoxification.detoxification genes ͉ in situ hybridization ͉ insecticide resistance ͉ multigene family ͉ RNAi

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Genomic analysis of detoxification genes in the mosquito Aedes aegypti

Cited by 310 publications

References 41 publications

Identifying genomic changes associated with insecticide resistance in the dengue mosquito Aedes aegypti by deep targeted sequencing

Identifying genomic changes associated with insecticide resistance in the dengue mosquito Aedes aegypti by deep targeted sequencing

Multiple insecticide resistance in Aedes aegypti (Diptera: Culicidae) populations compromises the effectiveness of dengue vector control in French Guiana

Characterization of Drosophila melanogaster cytochrome P450 genes

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