Genomic analysis of carbapenemase-producing Enterobacteriaceae in Queensland reveals widespread transmission of bla
            IMP-4 on an IncHI2 plasmid

Roberts, Leah W.; Catchpoole, Elizabeth; Jennison, Amy V.; Bergh, Haakon; Hume, Anna; Heney, Claire; George, Narelle; Paterson, David L.; Schembri, Mark A.; Beatson, Scott A.; Harris, Patrick N A

doi:10.1099/mgen.0.000321

Cited by 24 publications

(29 citation statements)

References 42 publications

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“…The ter-type 2 operons were located on the IncHI2 plasmids of E. coli strains, and were also found on the IncHI2 plasmids carried by Y. pseudotuberculosis and K. pneumoniae (Supplementary Figure 2), suggesting that this type of operon was horizontally transferred in E. coli as well as within the Enterobacteriaceae through plasmids. The conjugative IncHI2 plasmid is widely found in Enterobacteriaceae and is known to mediate various drug resistance genes (Herrera-León et al, 2011;Roberts et al, 2020). In fact, the plasmids found to harbor the ter-type 2 operons in this study carried three or more drug resistance genes (Figure 5).…”

Section: Discussionmentioning

confidence: 69%

Diversity of the Tellurite Resistance Gene Operon in Escherichia coli

et al. 2021

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Tellurite is highly toxic to most bacteria owing to its strong oxidative ability. However, some bacteria demonstrate tellurite resistance. In particular, some Escherichia coli strains, including Shiga toxin-producing E. coli O157:H7, are known to be resistant to tellurite. This resistance is involved in ter operon, which is usually located on a prophage-like element of the chromosome. The characteristics of the ter operon have been investigated mainly by genome analysis of pathogenic E. coli; however, the distribution and structural characteristics of the ter operon in other E. coli are almost unknown. To clarify these points, we examined 106 E. coli strains carrying the ter operon from various animals. The draft genomes of 34 representative strains revealed that ter operons were clearly classified into four subtypes, ter-type 1–4, at the nucleotide sequence level. Complete genomic sequences revealed that operons belonging to three ter-types (1, 3, and 4) were located on the prophage-like elements on the chromosome, whereas the ter-type 2 operon was located on the IncHI2 plasmid. The positions of the tRNASer, tRNAMet, and tRNAPhe indicated the insertion sites of elements carrying the ter operons. Using the PCR method developed in this study, 106 strains were classified as type 1 (n = 66), 2 (n = 13), 3 (n = 8), and 4 (n = 17), and two strains carried both types 1 and 2. Furthermore, significant differences in the minimum inhibitory concentration (MIC) of tellurite were observed between strains carrying ter-type 4 and the others (p < 0.05). The ter-type was also closely related to the isolation source, with types 2 and 4 associated with chickens and deer, respectively. This study provided new insights related not only to genetic characteristics of the ter operons, but also to phenotypic and ecological characteristics that may be related to the diversity of the operon.

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Section: Discussionmentioning

confidence: 69%

Diversity of the Tellurite Resistance Gene Operon in Escherichia coli

et al. 2021

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“…Because of the physical proximity of these ARGs and the presence of two bordering IS903 copies, the ARG locus-2 of pCM18-216 has the potential to facilitate the simultaneous horizontal gene transfer of the ESBL gene bla SHV-12 and the fluoroquinolone resistance gene qnr B2, along with other antimicrobial resistance genes, through a single transposition event. In Australia, bla IMP-4 genes have been associated with IncHI2 plasmids carried by E. hormaechei with various MLST profiles, but only two ST110 isolates [ 53 ]. These antimicrobials are considered of very high importance and their use in companion animals is not generally recommended ( https://vetantibiotics.fvas.unimelb.edu.au/ ).…”

Section: Discussionmentioning

confidence: 99%

Colonization of a hand washing sink in a veterinary hospital by an Enterobacter hormaechei strain carrying multiple resistances to high importance antimicrobials

Kamathewatta¹,

Bushell²,

Rafa³

et al. 2020

Antimicrob Resist Infect Control

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Background Hospital intensive care units (ICUs) are known reservoirs of multidrug resistant nosocomial bacteria. Targeted environmental monitoring of these organisms in health care facilities can strengthen infection control procedures. A routine surveillance of extended spectrum beta-lactamase (ESBL) producers in a large Australian veterinary teaching hospital detected the opportunistic pathogen Enterobacter hormaechei in a hand washing sink of the ICU. The organism persisted for several weeks, despite two disinfection attempts. Four isolates were characterized in this study. Methods Brilliance-ESBL selective plates were inoculated from environmental swabs collected throughout the hospital. Presumptive identification was done by conventional biochemistry. Genomes of multidrug resistant Enterobacter were entirely sequenced with Illumina and Nanopore platforms. Phylogenetic markers, mobile genetic elements and antimicrobial resistance genes were identified in silico. Antibiograms of isolates and transconjugants were established with Sensititre microdilution plates. Results The isolates possessed a chromosomal Tn7-associated silver/copper resistance locus and a large IncH12 conjugative plasmid encoding resistance against tellurium, arsenic, mercury and nine classes of antimicrobials. Clusters of antimicrobial resistance genes were associated with class 1 integrons and IS26, IS903 and ISCR transposable elements. The blaSHV-12, qnrB2 and mcr-9.1 genes, respectively conferring resistance to cephalosporins, quinolones and colistin, were present in a locus flanked by two IS903 copies. ESBL production and enrofloxacin resistance were confirmed phenotypically. The isolates appeared susceptible to colistin, possibly reflecting the inducible nature of mcr-9.1. Conclusions The persistence of this strain in the veterinary hospital represented a risk of further accumulation and dissemination of antimicrobial resistance, prompting a thorough disinfection of the ICU. The organism was not recovered from subsequent environmental swabs, and nosocomial Enterobacter infections were not observed in the hospital during that period. This study shows that targeted routine environmental surveillance programs to track organisms with major resistance phenotypes, coupled with disinfection procedures and follow-up microbiological cultures are useful to control these risks in sensitive areas of large veterinary hospitals.

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“…Reports on the bla NDM-5 -carrying IncHI2 plasmid are scarce. Previous reports have also suggested that the IncHI2 plasmid was considered to be an important vehicle in the spread of carbapenem resistance genes such as bla NDM-1 , bla NDM-4 , bla NDM-9 and bla IMP-4 among Enterobacteriaceae from human and animals [ 13 , 23 , 24 ]. Since the discovery of mcr-1 gene in 2015 that threatens the effectiveness of polymyxins, the IncHI2 plasmid that carried mcr-1 has been found in Enterobacteria from a variety of sources, including humans [ 25 ], animals (cattle, pigs, chickens, ducks and turkeys) [ 26 , 27 ], food (chicken, beef, pork, milk and vegetables) [ 28 , 29 ] and the environment (farm soil and wastewater) [ 30 ] in several countries around the world.…”

Section: Discussionmentioning

confidence: 99%

Emergence of IncHI2 Plasmid-Harboring blaNDM-5 from Porcine Escherichia coli Isolates in Guangdong, China

et al. 2021

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Carbapenem resistance has posed potential harmful risks to human and animals. The objectives of this study were to understand the prevalence of blaNDM-5 in pigs and investigate the molecular characteristics of NDM-5-producing Escherichia coli isolates in Guangdong province in China. Carbapenem-resistant E. coli isolates were isolated from pigs and obtained using MacConkey plates containing 0.5 mg/L meropenem. Conjugation assay and antimicrobial susceptibility testing were conducted for the isolates and their transconjugants. Whole-genome sequence (WGS) was used to analyze the plasmid genetic feature. A total of five blaNDM-5-carrying E. coli isolates were obtained in the present investigations. They belonged to five ST types. The blaNDM-5 genes were found to be in IncX3 and IncHI2 plasmid. The IncX3 plasmid was 46,161 bp in size and identical to other reports. IncHI2 plasmid was 246,593 bp in size and similar to other IncHI2-ST3 plasmids. It consisted of a typical IncHI2 plasmid backbone region and a multiresistance region (MRR). The blaNDM-5 was closely associated with the IS3000-ISAba125-blaNDM-5-bleMBL-trpF-tat-IS26 unit. We first reported the blaNDM-5-carrying IncHI2 in E. coli isolates recovered from pigs and revealed the molecular characterization. Continued surveillance for the dissemination of blaNDM-5 among food-producing animals is required.

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Genomic analysis of carbapenemase-producing Enterobacteriaceae in Queensland reveals widespread transmission of bla IMP-4 on an IncHI2 plasmid

Cited by 24 publications

References 42 publications

Diversity of the Tellurite Resistance Gene Operon in Escherichia coli

Diversity of the Tellurite Resistance Gene Operon in Escherichia coli

Colonization of a hand washing sink in a veterinary hospital by an Enterobacter hormaechei strain carrying multiple resistances to high importance antimicrobials

Emergence of IncHI2 Plasmid-Harboring blaNDM-5 from Porcine Escherichia coli Isolates in Guangdong, China

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