Genomic analysis of a lignocellulose degrading strain from the underexplored genus Meridianimaribacter

Abstract

The genus Meridianimaribacter is one of the least-studied genera within Cytophaga-Flavobacteria. To date, no genomic analysis of Meridianimaribacter has been reported. In this study, Meridianimar ibacter sp. strain CL38, a lignocellulose degrading halophile was isolated from mangrove soil. The genome of strain CL38 was sequenced and analyzed. The assembled genome contains 17 contigs with 3.33 Mbp, a GC content of 33.13% and a total of 2982 genes predicted. Lignocellulose degrading enzymes such as cellulases (GH3, 5, 9, 16, 74 and 144), xylanases (GH43 and CE4) and mannanases (GH5, 26, 27 and 130) are encoded in the genome. Furthermore, strain CL38 demonstrated its ability to decompose empty fruit bunch, a lignocellulosic waste residue arising from palm oil industry. The genome information coupled with experimental studies confirmed the ability of strain CL38 to degrade lignocellulosic biomass. Therefore, Meridianimar ibacter sp. strain CL38, with its halotolerance, could be useful for seawater based lignocellulosic biorefining.

Genome sequence of an uncharted halophilic bacterium Robertkochia marina with deciphering its phosphate-solubilizing ability

“…Halophiles are highly specialized organisms that thrive in hypersaline environments [1,2]. They possess the ability to produce enzymes with high biotechnological potential including proteases, amylases, cellulases, lipases, and phosphatases for industrial applications [3][4][5][6][7][8][9][10][11]. For instance, halophilic bacteria could enhance phosphate uptake by plants through solubilizing the insoluble phosphate into soluble forms by producing alkaline phosphatase [12].…”

A Review on Bacterial Contribution to Lignocellulose Breakdown into Useful Bio-Products

“…These methods allow for scarcely-studied species to be observed and more knowledge of their lignocellulolytic potential gathered. A lignocellulose degrading bacteria from the underexplored genus Meridianimaribacter was studied for lignocellulolytic properties and showed promising enzymatic activity [ 137 ]. Metagenomic analysis of the bacterial community in Vietnamese native goat rumen revealed the structure and diversity of lignocellulolytic bacteria in that microbial community and an understanding of lignocellulolytic functions.…”

Robertkochia solimangrovi sp. nov., isolated from mangrove soil, and emended description of the genus Robertkochia

“…The pH was verified after autoclaving. To test NaCl tolerance and optimal concentration, the bacteria were grown in a medium containing yeast extract (1.0 g l −1 ), peptone (5.0 g l −1 ), MgCl 2 (5.0 g l −1 ), MgSO 4 ·7H 2 O (2.0 g l −1 ), CaCl 2 (0.5 g l −1 ), KCl (1.0 g l −1 ) and NaCl (0, 0.5, 1–11 %, w/v) [ 10 ].…”

“…Due to periodic tidal flats, drastic changes in salinity and nutrient availability of the mangrove environment make it a unique ecosystem [7]. Free-living and symbiotic bacteria in such environments were found to play essential roles in maintaining mangrove ecosystem by, for example, recycling organic matter and biotransformation of minerals [8][9][10]. It was estimated that less than 5 % of species in mangrove environments have been described so far [11].…”