Genomic Analyses of Weissella cibaria W25, a Potential Bacteriocin-Producing Strain Isolated from Pasture in Campos das Vertentes, Minas Gerais, Brazil

Teixeira, Camila Gonçalves; Rodrigues, Rafaela da Silva; Yamatogi, Ricardo Seiti; Lucau-Danila, Anca; Drider, Djamel; Nero, Luís Augusto; Carvalho, Antônio Fernandes de

doi:10.3390/microorganisms10020314

Cited by 8 publications

(8 citation statements)

References 30 publications

(32 reference statements)

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“…Culture-independent approaches including PCR-DGGE and next-generation sequencing approaches such as metagenetics and metagenomics have allowed the detection of weissellas in various ecological niches ( Table 2 ). Whole-genome sequencing is another approach that is being widely used to identify and characterize weissellas (Benomar et al, 2011 ; Kim et al, 2011 ; Amari et al, 2012 ; Lee J. H. et al, 2012 ; Figueiredo et al, 2014a , b , 2015 ; Tanizawa et al, 2014 ; Malik et al, 2016 ; Heng et al, 2017 ; Ku et al, 2017 ; Li et al, 2017 ; Du et al, 2018 ; Garcia-Cancino et al, 2019 ; Kwak et al, 2019 ; Panthee et al, 2019 ; Lin et al, 2020 ; Månberger et al, 2020 ; Baugh et al, 2021 ; Contente et al, 2021 ; Jang et al, 2021 ; Patrone et al, 2021 ; Yuan et al, 2021 ; Apostolakos et al, 2022 ; Fanelli et al, 2022 ; Fukuda and Nolasco-Hipolito, 2022 ; Surachat et al, 2022 ; Teixeira et al, 2022 ).…”

Section: Detection and Typing Of Weissellamentioning

confidence: 99%

“…Teixeira et al ( 2022 ) sequenced the genome of W. cibaria W25, isolated from a Brazilian pasture samples of a Brazilian dairy farm, and found that this strain had the possibility of producing two different bacteriocins. Thereafter, the same authors (Teixeira et al, 2023 ) demonstrated a putative bacteriocinogenic activity of W. cibaria W25 against Salmonella enterica Newport, Kocuria rhizophila, Listeria innocua , and E. coli strains.…”

Section: An Update Of the Probiotic Potential Of Weissella ...mentioning

confidence: 99%

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The Weissella and Periweissella genera: up-to-date taxonomy, ecology, safety, biotechnological, and probiotic potential

Fusco,

Chieffi,

Fanelli

et al. 2023

Front. Microbiol.

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Bacteria belonging to the genera Weissella and Periweissella are lactic acid bacteria, which emerged in the last decades for their probiotic and biotechnological potential. In 2015, an article reviewing the scientific literature till that date on the taxonomy, ecology, and biotechnological potential of the Weissella genus was published. Since then, the number of studies on this genus has increased enormously, several novel species have been discovered, the taxonomy of the genus underwent changes and new insights into the safety, and biotechnological and probiotic potential of weissellas and periweissellas could be gained. Here, we provide an updated overview (from 2015 until today) of the taxonomy, ecology, safety, biotechnological, and probiotic potential of these lactic acid bacteria.

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Section: Detection and Typing Of Weissellamentioning

confidence: 99%

Section: An Update Of the Probiotic Potential Of Weissella ...mentioning

confidence: 99%

The Weissella and Periweissella genera: up-to-date taxonomy, ecology, safety, biotechnological, and probiotic potential

Fusco,

Chieffi,

Fanelli

et al. 2023

Front. Microbiol.

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Section: Genome Sequencing Assembly and Annotationmentioning

confidence: 99%

“…The genome sequence of the W25 strain was previously deposited in GenBank under accession number JAFNKE000000000 (Teixeira et al, 2022). The whole genomes of W. cibaria W21 and W42 were sequenced, trimmed, and assembled as recently performed for the W25 strain (Teixeira et al, 2022). Gene prediction and annotation were performed using the Rapid Prokaryotic Genome Annotation (PROKKA) v. 1.14.5 (Seemann, 2014), executed with default parameters, and also performed by RAST automated web server (Aziz et al, 2008).…”

Section: Genome Sequencing Assembly and Annotationmentioning

confidence: 99%

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Antimicrobial properties of Weissella cibaria strains isolated from Campos Das Vertentes, Minas Gerais, Brazil and its potential use as probiotics and bacteriocins producers

Teixeira¹

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The genus Weissella is composed of bacteria belonging to the group known as lactic acid bacteria (LAB), are autochthonous to both dairy and non-dairy environments and many strains can produce various compounds of interest to the food industry. This work began with deep research regarding the use of Weissella strains for the food and pharmaceutical industry and the probiotic potential of this genus. A large number of scientific studies reported and support the use of Weissella strains in this field due to their health-promoting properties and technological features for the food industry. This work continues with the study of the biodiversity of Weissella strains isolated in the regions producing artisanal cheeses in Brazil. For this, the analysis of PFGE and Rep- PCR was carried out and it was observed that each study region represents a specific ecological niche. After, the antimicrobial potential of some representative strains was analyzed against the most common foodborne pathogens. Most of the isolates tested showed good inhibition of the pathogens. Also, the technological potential of thirteen strains was evaluated. Some strains showed positive results for most of the tests performed. After, three strains with good inhibitory activity were selected. The genomes ware sequenced and assembled. With the first strain, Weissella cibaria W25 has carried out a comprehensive and comparative genomic analysis with W. cibaria 110, known to pro-duce the weissellicin 110 bacteriocin, and four other non- bacteriocin-producing W. cibaria strains. The analyses showed that the strain W25 has its unique protein cluster genes which can be related to bacteriocins genes indicated by AntiSmash, confirming the possibility of producing two different bacteriocins. Based on these results the genome of the three selected strains was deeply analyzed. The presence of genes for secondary metabolites was investigated and they have a common region in the genome with a putative bacteriocin identified as Bacteriocin_IIc. This putative bacteriocin has no similarity with other bacteriocins from the genus Weissella so far described. Finally, the safety of using these three strains for humanand/or animal consumption and their capacity to be used as probiotics were analyzed. All three strains have no acquired antimicrobial resistance genes and no mobile elements in the genome. W. cibaria strains were resistant to vancomycin which is intrinsic to this genus, had no hemolytic activity, and no one of the known virulence factors was detected. They possess the defense system CRISPR-Cas. Besides they didn’t present any inflammation or cytotoxicity capacity. W. cibaria W21 demonstrates excellent results of adhesion to intestinal cells and exclusion of S. aureus MRSA SA1. However, none of the three strains survived completely through the simulated gastrointestinal tract, but some protective ways can be further studied. In conclusion, this work allowed us to understand the diversity and distribution of Weissella species in different regions producing artisanal cheeses in Brazil. It also showed that some of them have a favorable technological and safety potential for future uses in the food industry or as a probiotic culture. Keywords: Weissella. Technological properties. Antimicrobial activity. Bacteriocins.

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Weissella and the two Janus faces of the genus

Kim

Yang

Kim

2023

Appl Microbiol Biotechnol

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Genomic Analyses of Weissella cibaria W25, a Potential Bacteriocin-Producing Strain Isolated from Pasture in Campos das Vertentes, Minas Gerais, Brazil

Cited by 8 publications

References 30 publications

The Weissella and Periweissella genera: up-to-date taxonomy, ecology, safety, biotechnological, and probiotic potential

The Weissella and Periweissella genera: up-to-date taxonomy, ecology, safety, biotechnological, and probiotic potential

Antimicrobial properties of Weissella cibaria strains isolated from Campos Das Vertentes, Minas Gerais, Brazil and its potential use as probiotics and bacteriocins producers

Weissella and the two Janus faces of the genus

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