Genomic Analyses of Two Populations of Ageniaspis citricola (Hymenoptera: Encyrtidae) Suggest That a Cryptic Species May Exist

Hoy, Marjorie A.; Jeyaprakash, Ayyamperumal; Morakote, Rut; Lo, P. K. C.; Nguyen, Ru

doi:10.1006/bcon.1999.0775

Cited by 35 publications

(21 citation statements)

References 22 publications

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“…Schonrogge et al (2002) singled out koinobiont parasitoids, along with true parasites and social parasites, as being particularly susceptible to cryptic speciation. Binodoxys species are koinobiont parasitoids, as are many parasitoid groups that have been found to contain cryptic species complexes (Hoy et al 2000;Kankare et al 2005a,b;Quicke et al 2006;Smith et al 2006Smith et al , 2007Smith et al , 2008Heraty et al 2007;Kathirithamby 2009). However, cryptic species have been found in idiobiont parasitoid lineages as well (Clarke 1993, Fernando and Walter 1997, Woolley et al 1994, Heimpel et al 1997, Pinto et al 2003, Bernardo et al 2008, and in members of the genus Eretmocerus (Hymenoptera: Aphelindiae), which are facultative koino/idiobionts (Hunter et al 1996, Jones andGreenberg 1998).…”

Section: Discussionmentioning

confidence: 99%

Cryptic Species of Parasitoids Attacking the Soybean Aphid (Hemiptera: Aphididae) in Asia: Binodoxys communis and Binodoxys koreanus (Hymenoptera: Braconidae: Aphidiinae)

Desneux

Starý

Delebecque

et al. 2009

Annals of the Entomological Society of America

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Collections of parasitoids attacking the soybean aphid, Aphis glycines Matsumura (Hemiptera: Aphididae), in South Korea yielded specimens that were originally identified as Binodoxys communis (Gahan) (Hymenoptera: Braconidae). We report here on laboratory studies indicating that this population is actually a previously unknown species of Binodoxys. Four classes of comparisons were made between the Korean population and a Chinese population that also had been identified as B. communis. The comparisons included 1) mating trials coupled with behavioral observations and spermathecal examinations, 2) assessment of nucleotide divergence at two mitochondrial and two nuclear gene loci, 3) patterns of host use, and 4) reassessment of morphological characters. These studies revealed premating reproductive isolation of the two populations and minor nucleotide differences in mitochondrial cytochrome oxidase I sequences and nuclear internal transcriber spacer 1 sequences, providing strong indications that they are different species. Subtle morphological differences also were discovered that confirmed that the Chinese population corresponds to B. communis, whereas the Korean population does not. We propose the name Binodoxys koreanus StarÝ, sp. n. for the Korean population. The two species exhibited similar host ranges in the laboratory, the most notable exception being that B. koreanus, sp. n. is better able to develop in a population of Aphis craccivora Koch that harbors the bacterial endosymbiont Hamiltonella defensa Moran, which seems to strongly interfere with the development of B. communis. We discuss the implications of our results for biological control introductions against the soybean aphid in North America.

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Section: Discussionmentioning

confidence: 99%

Cryptic Species of Parasitoids Attacking the Soybean Aphid (Hemiptera: Aphididae) in Asia: Binodoxys communis and Binodoxys koreanus (Hymenoptera: Braconidae: Aphidiinae)

Desneux

Starý

Delebecque

et al. 2009

Annals of the Entomological Society of America

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“…To determine whether two colonies of Ageniaspis citricola Logvinovskaya that differ slightly in their behaviour and biology were cryptic species, Hoy et al. (2000) employed RAPD‐PCR.…”

Section: Molecular Diagnostics In Parasitoid Researchmentioning

confidence: 99%

Parasitoids, predators and PCR: the use of diagnostic molecular markers in biological control of Arthropods

Gariépy

Kuhlmann

Gillott

et al. 2007

J Applied Entomology

171

144

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Abstract:The polymerase chain reaction (PCR) revolutionized the field of diagnostics, and today it has routine applications in medical, veterinary, forensic and botanical sciences. The fields of biological control and insect pest management have generally been slow to adopt PCR-based diagnostics in comparison with other fields of science. However, there has been increasing interest in the use of molecular diagnostic tools in arthropod biological control. In applied entomology, molecular techniques have generally been used for insect identification and systematics; however, PCR-based techniques are increasingly becoming recognized as valuable tools in ecological studies. Here, we review research that has used PCR-based techniques for parasitoid and predator/prey identification and detection, and place these studies in the context of their contributions to biological control of arthropods. The status and future directions of diagnostic molecular markers in applied entomology and insect pest management are also discussed.

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“…The ISSR-PCR DNA Þngerprinting method also readily distinguished the adult stages of E. diaspidicola and E. berlesei with two separate ISSR primers. Not sharing of bands is usually an indication of lack of gene ßow and reproductive isolation (Hoy et al 2000;de Leó n et al 2004ade Leó n et al , 2006bde Leó n et al , 2008. We have used this sensitive method extensively and have shown it to be very useful in uncovering species level divergences in a majority of cases, speciÞcally with egg parasitoids of sharpshooters belonging to the genus Gonatocerus Nees (de Leó n et al 2004a(de Leó n et al , 2006bde Leó n and Morgan 2007;Triapitsyn et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Molecular Markers Discriminate Closely Related Species <I>Encarsia diaspidicola</I> and <I>Encarsia berlesei</I> (Hymenoptera: Aphelinidae): Biocontrol Candidate Agents for White Peach Scale in Hawaii

León

Neumann

Follett

et al. 2010

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We genetically characterized Encarsia diapsidicola Silvestri and Encarsia berlesei Howard (Hymenoptera: Aphelinidae) by two molecular methods: phylogenetic analysis of the cytochrome oxidase subunit I gene (COI) and intersimple sequence repeat-polymerase chain reaction (ISSR-PCR) DNA fingerprinting. These two closely related endoparasitoids are candidate biological control agents for the white peach scale, Pseudaulacaspis pentagona Targioni-Tozetti (Hemiptera: Diaspididae), in Hawaii. We developed species-specific COI molecular markers that discriminated the two species, and we tested the utility of the E. diaspidicola-specific COI marker to detect parasitism of white peach scale. The COI sequence data uncovered 46-bp differences between the two Encarsia spp. The level of COI genetic divergence between the two species was 9.7%, and the two clustered into their own clade on a parismonious phylogram. ISSR-PCR readily discriminated the two Encarsia spp. because each was observed with fixed species-specific banding patterns. The COI molecular markers were specific for each species because cross-reactivity was not observed with nontarget species. The E. diaspidicola-specific COI markers were successful at detecting parasitism of white peach scale by E. diaspidicola by 24 h. Both molecular marker types successfully discriminated the two Encarsia spp., whereas the COI markers will be useful as tools to assess levels of parasitism in the field and to study competitive interactions between parasitoids.

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Genomic Analyses of Two Populations of Ageniaspis citricola (Hymenoptera: Encyrtidae) Suggest That a Cryptic Species May Exist

Cited by 35 publications

References 22 publications

Cryptic Species of Parasitoids Attacking the Soybean Aphid (Hemiptera: Aphididae) in Asia: Binodoxys communis and Binodoxys koreanus (Hymenoptera: Braconidae: Aphidiinae)

Cryptic Species of Parasitoids Attacking the Soybean Aphid (Hemiptera: Aphididae) in Asia: Binodoxys communis and Binodoxys koreanus (Hymenoptera: Braconidae: Aphidiinae)

Parasitoids, predators and PCR: the use of diagnostic molecular markers in biological control of Arthropods

Molecular Markers Discriminate Closely Related Species <I>Encarsia diaspidicola</I> and <I>Encarsia berlesei</I> (Hymenoptera: Aphelinidae): Biocontrol Candidate Agents for White Peach Scale in Hawaii

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