Genomic analyses of sodium channel α-subunit genes from strains of melon thrips, Thrips palmi, with different sensitivities to cypermethrin

Bao, Wen Xue; Kataoka, Yoko; Kohara, Yoko; Sonoda, Shoji

doi:10.1016/j.pestbp.2013.12.009

Cited by 4 publications

(4 citation statements)

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“…Pyrethroid resistance has been reported in thrips, such as T. tabaci, F. occidentalis, and T. palmi [20,35,42]. The M918T, L1014F, and T929I had been reported in several pyrethroidresistant T. tabaci strains in Japan [21].…”

Section: Discussionmentioning

confidence: 99%

Detection of Putative Mutation I873S in the Sodium Channel of Megalurothrips usitatus (Bagnall) Which May Be Associated with Pyrethroid Resistance

Gao

Qiu

et al. 2023

Insects

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Pyrethroid resistance of thrips has been reported in many countries, and knockdown resistance (kdr) has been identified as a main mechanism against pyrethroids in many insects. To characterize pyrethroid resistance in Megalurothrips usitatus from the Hainan Province of China, we conducted a biological assay and sequenced the voltage-gated sodium channel gene domain II from M. usitatus field populations. It showed high resistance to the pyrethroids for 2019 and 2020, in which LC50 to lambda-cyhalothrin of M. usitatus was 1683.521 mg/L from Sanya in 2020. The LC50 value of deltamethrin was lower in Haikou than in other locations, which mean the south of Hainan has higher resistance than the north of Hainan. Two mutations of I873S and V1015M were detected in the domain II region of the sodium channel in M. usitatus; however, the mutation frequency of V1015M was only 3.33% and that of I873S was 100%. One is homozygous and the other is a heterozygous mutant type. The three thrips-sensitive strains of sodium channel 873 are highly conserved in amino acids (isoleucine), while the M. usitatus pyrethroid-resistant strains are all serine, so I873S may be related to the resistance of M. usitatus to pyrethroids. The present study will contribute to the understanding of the evolution of pyrethroids resistance and contribute to the development of resistance management of M. usitatus in Hainan.

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Section: Discussionmentioning

confidence: 99%

Detection of Putative Mutation I873S in the Sodium Channel of Megalurothrips usitatus (Bagnall) Which May Be Associated with Pyrethroid Resistance

Gao

Qiu

et al. 2023

Insects

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“…T929I and T929V mutations were detected at a combined frequency of 100% in all of the populations tested and there was a complete absence of any wild‐type or T929C mutated alleles. Compared with T929V and T929C, 9 the role of T929I in pyrethroid resistance is considerably better documented in thrips species 9,29–32 . Given that there are no data to suggest that all three substitutions have the same role in resistance, future studies are needed to assess each mutation individually, by either running separate reactions or employing a higher order of multiplexing, in phenotypically characterized thrips field populations.…”

Section: Discussionmentioning

confidence: 99%

“…Compared with T929V and T929C, 9 the role of T929I in pyrethroid resistance is considerably better documented in thrips species. 9,[29][30][31][32] Given that there are no data to suggest that all three substitutions have the same role in resistance, future studies are needed to assess each mutation individually, by either running separate reactions or employing a higher order of multiplexing, in phenotypically characterized thrips field populations. The L1014F mutation was also present at frequencies ranging from 11.01% to 37.29%.…”

Section: Discussionmentioning

confidence: 99%

Molecular diagnostics for monitoring insecticide resistance in the western flower thrips Frankliniella occidentalis

Mavridis

Ilias

Papapostolou

et al. 2023

Pest Management Science

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BACKGROUND: Insecticide resistance has emerged in various western flower thrips (WFT) populations across the world, threatening the efficiency of chemical control applications. Elucidation of insecticide resistance mechanisms at the molecular level provides markers for the development of diagnostics to monitor the trait and support evidence-based resistance management.RESULTS: TaqMan and Droplet Digital polymerase chain reaction (ddPCR) diagnostics were developed and validated, against Sanger sequencing, in individual and pooled WFT samples respectively, for the G275E mutation (nicotinic acetylcholine receptor ⊍6 gene, nAChR ⊍6) associated with resistance to nAChR allosteric modulators, site I (spinosyns); L1014F, T929I, T929C and T292V mutations (voltage-gated sodium channel gene, vgsc) linked with pyrethroid resistance; and I1017M (chitin synthase 1 gene, chs1) conferring resistance to growth inhibitors affecting CHS1 (benzoylureas). The detection limits of ddPCR assays for mutant allelic frequencies (MAF) were in the range of 0.1%-0.2%. The assays were applied in nine WFT field populations from Crete, Greece. The G275E (MAF = 29.66%-100.0%), T929I and T929V (combined MAF = 100%), L1014F (MAF = 11.01%-37.29%), and I1017M (MAF = 17.74%-51.07%) mutations were present in all populations.CONCLUSION: The molecular diagnostics panel that was developed in this study can facilitate the quick and sensitive resistance monitoring of WFT populations at the molecular level, to support evidence-based insecticide resistance management strategies.

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“…The resistance of thrips to pyrethroids gradually increases with frequency and dosage (Tang et al, 2016). For example, T. palmi has developed resistance to cypermethrin at the time of invasion in Japan (Mullin & Scott, 1992) and moderate level resistance to λ‐pyrethroids (resistance ratio: 54.40; Bao et al, 2014). The strain in Shandong province of China has developed moderate levels of resistance to λ‐cyhalothrin (resistance ratio: 15.41; Zhang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Double sodium channel mutation, I265T/L1014F, is possibly related to pyrethroid‐resistant in Thrips palmi

Yuan

Jin

et al. 2023

Arch Insect Biochem Physiol

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Thrips palmi Karny (Thysanoptera: Thripidae) can harm a variety of agricultural crops and transmit plant viruses, causing heavy economic losses. In the Hainan province of China, pyrethroids were sprayed widely to control T. palmi, which leaded to resistance to pyrethroids in T. palmi. The bioassay has shown that the resistance ratio of T. palmi to pyrethroids increases annually. Resistance ratio to λ‐cyhalothrin has increased from 10.711 to 23.321 and to cypermethrin has increased from 5.507 to 23.051 for 3 years, 2020−2022. The double mutation (I265T/L1014F) was identified from the field strain for the first time, which were located in the domains I and II of the voltage‐gated sodium channel of T. palmi, respectively. The double mutation is probably the reason for the higher resistance of T. palmi in Hainan. The frequencies of the double mutation were 53.33% in HN2020, 70.00% in HN2021, and 96.67% in HN2022. Results indicated that T. palmi had developed different degrees of resistance to pyrethroids in Hainan. This study provides theoretical guidance for the use of insecticides in the field control of thrips.

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Genomic analyses of sodium channel α-subunit genes from strains of melon thrips, Thrips palmi, with different sensitivities to cypermethrin

Cited by 4 publications

References 20 publications

Detection of Putative Mutation I873S in the Sodium Channel of Megalurothrips usitatus (Bagnall) Which May Be Associated with Pyrethroid Resistance

Detection of Putative Mutation I873S in the Sodium Channel of Megalurothrips usitatus (Bagnall) Which May Be Associated with Pyrethroid Resistance

Molecular diagnostics for monitoring insecticide resistance in the western flower thrips Frankliniella occidentalis

Double sodium channel mutation, I265T/L1014F, is possibly related to pyrethroid‐resistant in Thrips palmi

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