Genomic analyses of KEL alleles in alloimmunized thalassemia patients from Iran

Sarihi, Reyhaneh; Amirizadeh, Naser; Oodi, Arezoo

doi:10.1016/j.transci.2020.102840

Cited by 2 publications

(1 citation statement)

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“…Ten cases were found to have discrepancies in the Kell blood group system that most of them (six cases) were related to the state of the K− k+ phenotype and KEL*01/KEL*02 genotype (Reyhaneh et al, 2020 ). Consequently, using molecular typing and accurate determination of K (KEL*01) antigen, searching for K‐negative RBC units in these patients, are not needed and saving a significant number of K‐negative RBC units.…”

Section: Discussionmentioning

confidence: 99%

Blood group genotyping in alloimmunized multi‐transfused thalassemia patients from Iran

Sarihi

Oodi

tehrani

et al. 2021

Molec Gen & Gen Med

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Objectives Serological methods may not be reliable for RBC antigen typing, especially in multi‐transfused patients. The blood group systems provoking the most severe transfusion reactions are mainly Rh, Kell, Kidd, and Duffy. We intended to determine the genotype of these blood group system antigens among Iranian alloimmunized thalassemia patients using molecular methods and compare the results with serological phenotyping. Methods Two hundred patients participated in this study. Blood group phenotype and genotype were determined using the serological method and PCR‐SSP, respectively. The genotypes of patients with incompatibility between phenotype and genotype were re‐evaluated by RFLP‐PCR and confirmed by DNA sequencing. Results Discrepancies between phenotype and genotype results were found in 132 alleles and 83 (41.5%) patients; however, there was complete accordance between the three genotyping methods. Most discrepancies were detected in Rh and Duffy systems with 47 and 45 cases, respectively, and the main discrepancy was in the FY*B/FY*B allele when serologically showed Fy(a+b+). All 39 undetermined phenotypes, due to mixed‐field reactions, were resolved by molecular genotyping. Conclusion Molecular genotyping is more reliable compared with the serological method, especially in multi‐transfused patients. Therefore, the addition of blood group genotyping to serological assays can lead to an antigen‐matched transfusion in these patients.

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Section: Discussionmentioning

confidence: 99%