Genomic adaptation of Pseudomonas strains to acidity and antibiotics in hydrothermal vents at Kolumbo submarine volcano, Greece

Bravakos, Panos; Mandalakis, Manolis; Nomikou, Paraskevi; Anastasiou, Thekla I.; Kristoffersen, Jon Bent; Stavroulaki, M.; Kilias, Stephanos P.; Kotoulas, Georgios; Magoulas, Antoniοs; Polymenakou, Paraskevi N.

doi:10.1038/s41598-020-79359-y

Cited by 10 publications

(12 citation statements)

References 67 publications

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“…Until the SANTORY observatory, sampling for microbial diversity was performed sporadically. Despite the vital role of microorganisms in hydrothermal vent ecosystems, only recently have bio-geochemical (Kilias et al, 2013;Christakis et al, 2018) and metagenomics and genomic investigations (Oulas et al, 2016;Mandalakis et al, 2019;Bravakos et al, 2021) been performed at Kolumbo volcano. These investigations revealed that both Kolumbo crater and Santorini caldera harbor highly complex prokaryotic communities (Oulas et al, 2016;Christakis et al, 2018) and microbes with an enhanced co-tolerance to acidity and antibiotics (Mandalakis et al, 2019;Bravakos et al, 2021).…”

Section: Expected Results-discussionmentioning

confidence: 99%

“…Despite the vital role of microorganisms in hydrothermal vent ecosystems, only recently have bio-geochemical (Kilias et al, 2013;Christakis et al, 2018) and metagenomics and genomic investigations (Oulas et al, 2016;Mandalakis et al, 2019;Bravakos et al, 2021) been performed at Kolumbo volcano. These investigations revealed that both Kolumbo crater and Santorini caldera harbor highly complex prokaryotic communities (Oulas et al, 2016;Christakis et al, 2018) and microbes with an enhanced co-tolerance to acidity and antibiotics (Mandalakis et al, 2019;Bravakos et al, 2021). The observatory will enable microbiologists to locate and revisit specific microbiological features of the vent field, to study the microbial communities' composition, structure and response to changes in the volcanic/hydrothermal system and to understand the physicochemical factors that shape the antibiotic resistome.…”

Section: Expected Results-discussionmentioning

confidence: 99%

“…x) Moreover, the associated chemosynthetic microbial biomes and the presence and risks of potential pathogens are poorly known (e.g., Oulas et al, 2016;Christakis et al, 2018;Mandalakis et al, 2019;Bravakos et al, 2021). Together with the geochemical and isotopic characterization, samplers for water and microbial mat collection will be also deployed at the vents and the collected samples will be further processed in the lab.…”

Section: Shore-based Mineralogical Geochemical and Biological Analysesmentioning

confidence: 99%

“…Hydrothermal activity also supports an astonishing diversity of seafloor vent ecosystems influencing global carbon and nutrient cycles (Van Dover et al, 2002;Sander and Koschinsky, 2011;Hawkes et al, 2015), aspects that have been widely studied in different geodynamic settings (Lupton et al, 1990;Taran et al, 1992;Lilley et al, 1993;Tsunogai et al, 1994;Butterfield et al, 1997;Lupton et al, 2006;Lupton et al, 2008;Lupton et al,2009;Caracausi et al, 2005a;Lan et al, 2010;Hannington et al, 2011;Kilias et al, 2013;Rizzo et al, 2016a;Rizzo et al, 2019;Bravakos et al, 2021). Most of the known modern seafloor hydrothermal systems, with their associated mineral deposits and chemosynthetic microbial biomes, occur at MORs and mature back-arc spreading centers, typically at water depths of 2000 to 4000m (e.g., Butterfield et al, 1990;Lilley et al, 1993;Lupton et al, 1999;Price and Giovanneli, 2017).…”

Section: Introductionmentioning

confidence: 99%

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SANTORY: SANTORini’s Seafloor Volcanic ObservatorY

et al. 2022

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Submarine hydrothermal systems along active volcanic ridges and arcs are highly dynamic, responding to both oceanographic (e.g., currents, tides) and deep-seated geological forcing (e.g., magma eruption, seismicity, hydrothermalism, and crustal deformation, etc.). In particular, volcanic and hydrothermal activity may also pose profoundly negative societal impacts (tsunamis, the release of climate-relevant gases and toxic metal(loid)s). These risks are particularly significant in shallow (<1000m) coastal environments, as demonstrated by the January 2022 submarine paroxysmal eruption by the Hunga Tonga-Hunga Ha’apai Volcano that destroyed part of the island, and the October 2011 submarine eruption of El Hierro (Canary Islands) that caused vigorous upwelling, floating lava bombs, and natural seawater acidification. Volcanic hazards may be posed by the Kolumbo submarine volcano, which is part of the subduction-related Hellenic Volcanic Arc at the intersection between the Eurasian and African tectonic plates. There, the Kolumbo submarine volcano, 7 km NE of Santorini and part of Santorini’s volcanic complex, hosts an active hydrothermal vent field (HVF) on its crater floor (~500m b.s.l.), which degasses boiling CO2–dominated fluids at high temperatures (~265°C) with a clear mantle signature. Kolumbo’s HVF hosts actively forming seafloor massive sulfide deposits with high contents of potentially toxic, volatile metal(loid)s (As, Sb, Pb, Ag, Hg, and Tl). The proximity to highly populated/tourist areas at Santorini poses significant risks. However, we have limited knowledge of the potential impacts of this type of magmatic and hydrothermal activity, including those from magmatic gases and seismicity. To better evaluate such risks the activity of the submarine system must be continuously monitored with multidisciplinary and high resolution instrumentation as part of an in-situ observatory supported by discrete sampling and measurements. This paper is a design study that describes a new long-term seafloor observatory that will be installed within the Kolumbo volcano, including cutting-edge and innovative marine-technology that integrates hyperspectral imaging, temperature sensors, a radiation spectrometer, fluid/gas samplers, and pressure gauges. These instruments will be integrated into a hazard monitoring platform aimed at identifying the precursors of potentially disastrous explosive volcanic eruptions, earthquakes, landslides of the hydrothermally weakened volcanic edifice and the release of potentially toxic elements into the water column.

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Section: Expected Results-discussionmentioning

confidence: 99%

Section: Expected Results-discussionmentioning

confidence: 99%

Section: Shore-based Mineralogical Geochemical and Biological Analysesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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SANTORY: SANTORini’s Seafloor Volcanic ObservatorY

et al. 2022

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“…In addition, microorganisms living in hydrothermal vents may tend to enrich various stress tolerance genes via HGT, which could be helpful for them to adapt to harsh environmental conditions (e.g., high temperatures and toxic gases) [33]. It has been suggested that ARGs are beneficial for thermal microorganisms not only in terms of coping with selective environmental stresses [34], but also for competing with other species [35]. Some prior investigations have shown that ARGs were ubiquitous in diverse pristine environments, including plateau lake sediments [36], polar soils [37], hot springs [38,39] and deep-sea hydrothermal vents [35]; however, it is difficult to compare the results of different studies as different methodologies were used for ARG analysis in different studies.…”

Section: Introductionmentioning

confidence: 99%

A Comprehensive Profile of Antibiotic Resistance Genes in the Water Column of a Shallow-Sea Hydrothermal Vent Ecosystem

Zhang

Yu-fang

et al. 2022

Sustainability

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Antibiotic resistance genes (ARGs) became an emerging contaminant, and were found to accumulate in natural and man-made environments. A comprehensive understanding of the diversity and abundance of ARGs in pristine environments is critical for defining the baseline levels of environmental ARGs. However, there is limited information available on the ARG profiles of pristine environments, especially for shallow-sea hydrothermal vents ecosystems. Here, we combined 16S rRNA gene full-length amplicon sequencing and high-throughput quantitative PCR (HT-qPCR) to study the bacterial communities, and ARG abundance and diversity in the shallow-sea hydrothermal vent ecosystem of the Kueishantao Islet. The results of the 16S rRNA gene amplicon sequencing showed that several sulfur-cycling related bacterial genera, including Thiomicrorhabdus, Thioreductor, Sulfurovum, Sulfurimonas and Lebetimonas, dominated in the water column of the shallow-sea system. Temperature was the significant factor shaping the bacterial communities. The results of HT-qPCR analysis showed that the Kueishantao shallow-sea system harbored the lowest diversity (average 10 ARG subtypes) and abundance (average 1.0 × 10−3 copy per bacterial cell) of ARGs compared with other pristine (i.e., Tibet lake sediments, marine water and sediments) and anthropogenic-disturbed (i.e., drinking water reservoirs, urban ponds and wastewater treatment plants) environments. Procrustes analysis demonstrated a concordant pattern between the compositions of bacterial communities and ARGs in the shallow-sea system, while variation partition analysis revealed that the shared effects of physicochemical and bacterial communities explained >80% of the variation in the composition of ARGs. These results suggest that the vent bacterial communities and local environmental factors played an important role in shaping the distribution of the ARG profiles. Our study provides the first comprehensive overview of the background level of ARGs in a shallow-sea hydrothermal vent ecosystem.

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Microbial Life in the Aegean Sea

Meziti

Kormas

2022

The Handbook of Environmental Chemistry

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Genomic adaptation of Pseudomonas strains to acidity and antibiotics in hydrothermal vents at Kolumbo submarine volcano, Greece

Cited by 10 publications

References 67 publications

SANTORY: SANTORini’s Seafloor Volcanic ObservatorY

SANTORY: SANTORini’s Seafloor Volcanic ObservatorY

A Comprehensive Profile of Antibiotic Resistance Genes in the Water Column of a Shallow-Sea Hydrothermal Vent Ecosystem

Microbial Life in the Aegean Sea

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