Genomewide screening for genes involved in biofilm formation and miconazole susceptibility inSaccharomyces cerevisiae

Abstract

Infections related to fungal biofilms are difficult to treat due to the reduced susceptibility of sessile cells to most antifungal agents. Previous research has shown that 1-10% of sessile Candida cells survive treatment with high doses of miconazole (a fungicidal imidazole). The aim of this study was to identify genes involved in fungal biofilm formation and to unravel the mechanisms of resistance of these biofilms to miconazole. To this end, a screening of a Saccharomyces cerevisiae deletion mutant bank was carried out. Our results revealed that genes involved in peroxisomal transport and the biogenesis of the respiratory chain complex IV play an essential role in biofilm formation. On the other hand, genes involved in transcription and peroxisomal and mitochondrial organization seem to highly influence the susceptibility to miconazole of yeast biofilms. Additionally, our data confirm previous findings on genes involved in biofilm formation and in general stress responses. Our data suggest the involvement of peroxisomes in biofilm formation and miconazole resistance in fungal biofilms.

Crowded growth leads to the spontaneous evolution of semistable coexistence in laboratory yeast populations

“…Instead, the association between adherence and slower growth may be incidental to mutations disrupting the ergosterol pathway. Previous studies have shown that such mutations reduce growth rate (38) and that deletions of ERG3 and ERG4 can increase and decrease adherence, respectively (39). Most likely, A mutants are more hydrophobic, because adherence to polystyrene is itself a measure of cell-surface hydrophobicity (40,41).…”

Antifungal activity of cathelicidin peptides against planktonic and biofilm cultures of Candida species isolated from vaginal infections

“…biofilms [79]. The underlying mechanism seems rather complex and is still the subject of active research [80]. In the present study, a significant decrease in metabolic activity (Fig.…”

Differential Proteome Analysis of a Flor Yeast Strain under Biofilm Formation

“…Based on the large differences in contents between BFC and NBFC, its functions and the fact that until now it has only been located in the extracellular region [43], Ygp1p may be a constituent of the extracellular matrix, the composition of which remains unknown [44]. A high content in protein Pst2p, but similar under BFC (0.55 mol %) and NBFC (0.42 mol %) here, was previously correlated with the formation of a yeast biofilm [41]. …”

“…By contrast, Ygp1p exhibited the largest differences between BFC and NBFC. Ygp1p is an extensively N -glycosylated cell wall-related secretory protein potentially involved in cellular adaptations prior to the stationary phase, cellular amino acid metabolism, cell wall assembly and, also, possibly, biofilm formation [41]. Destruelle et al [42] observed YGP1 induction in response to nutrient limitation and repression by high glucose concentrations.…”