Genomewide identification and analysis of the OSCA gene family in barley (Hordeum vulgare L.)

Cai, Qian; Wang, Yuexue; Ni, Shoufei; Mu, Jingjiao; Liu, Mengdi; Wang, Yanfang; Zhao, Yanhong

doi:10.1007/s12041-022-01375-w

Cited by 3 publications

(5 citation statements)

References 50 publications

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“…The RSN1_7TM functional domain is the signature domain of the OSCA family. Our results showed that all MsOSCA proteins contained the RSN1_7TM functional domain (Figure 2), which was consistent with maize (Cao et al, 2020), barley (Cai et al, 2022) and legumes (Chakraborty et al, 2023). In addition, some proteins (MsOSCA1/-2/-3/-4/-6/-7/-9/-10/-13) of the MsOSCA family contained RSN1_TM and PHM7_cyt functional domains, similar to wheat (TaOSCA-1/-2/-3/-4/-14/-27) (Tong et al, 2021).…”

Section: Discussionsupporting

confidence: 82%

“…In this study, a total of 14 OSCA genes were identified in the alfalfa genome (Table 1), and this number was different from the number of OSCA genes in Arabidopsis (Yuan et al, 2014). The number of OSCA genes varied significantly between homologous species in systematic genome-wide analysis, which typically ranged from 11 to 15 in plants (Cai et al, 2022). However, 42 OSCA genes were identified in the wheat genome, far more than in any other species (Tong et al, 2021), indicating that the wheat OSCA gene family has undergone fragment and tandem duplication events during evolution.…”

Section: Discussionmentioning

confidence: 74%

“…MsOSCAs were expressed in both roots and leaves, indicating that they might play important roles in vegetative growth. However, the expression of MsOSCA12 was not detected in the stems and petioles (Figure 6), and transcriptome data analysis showed that MsOSCA12 was only expressed in flowers (Figure 5), so we hypothesized that the gene may be related to circadian rhythm, osmotic stress sensing site and reproductive growth and development (Cai et al, 2022;Miao et al, 2022). MsOSCA8 was significantly more highly expressed in roots than in other tissues, which may be closely related to the perception and response of osmotic stress.…”

Section: Discussionmentioning

confidence: 99%

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Genome-wide investigation and expression analysis of OSCA gene family in response to abiotic stress in alfalfa

Li,

Wang,

et al. 2023

Front. Plant Sci.

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Alfalfa is an excellent leguminous forage crop that is widely cultivated worldwide, but its yield and quality are often affected by drought and soil salinization. Hyperosmolality-gated calcium-permeable channel (OSCA) proteins are hyperosmotic calcium ion (Ca2+) receptors that play an essential role in regulating plant growth, development, and abiotic stress responses. However, no systematic analysis of the OSCA gene family has been conducted in alfalfa. In this study, a total of 14 OSCA genes were identified from the alfalfa genome and classified into three groups based on their sequence composition and phylogenetic relationships. Gene structure, conserved motifs and functional domain prediction showed that all MsOSCA genes had the same functional domain DUF221. Cis-acting element analysis showed that MsOSCA genes had many cis-regulatory elements in response to abiotic or biotic stresses and hormones. Tissue expression pattern analysis demonstrated that the MsOSCA genes had tissue-specific expression; for example, MsOSCA12 was only expressed in roots and leaves but not in stem and petiole tissues. Furthermore, RT–qPCR results indicated that the expression of MsOSCA genes was induced by abiotic stress (drought and salt) and hormones (JA, SA, and ABA). In particular, the expression levels of MsOSCA3, MsOSCA5, MsOSCA12 and MsOSCA13 were significantly increased under drought and salt stress, and MsOSCA7, MsOSCA10, MsOSCA12 and MsOSCA13 genes exhibited significant upregulation under plant hormone treatments, indicating that these genes play a positive role in drought, salt and hormone responses. Subcellular localization results showed that the MsOSCA3 protein was localized on the plasma membrane. This study provides a basis for understanding the biological information and further functional analysis of the MsOSCA gene family and provides candidate genes for stress resistance breeding in alfalfa.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

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Genome-wide investigation and expression analysis of OSCA gene family in response to abiotic stress in alfalfa

Li,

Wang,

et al. 2023

Front. Plant Sci.

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“…Since the discovery of the OSCA family, its members have been identified and characterized in many species [ 12 , 13 , 15 , 16 ]. The protein structure and biological function of OSCA1 have been reported because it plays an important role in plant sensing of hyperosmolality stress [ 9 , 10 , 11 , 17 ].…”

Section: Discussionmentioning

confidence: 99%

“…The OSCA gene (reduced hyperosmolality-induced [Ca 2+ ] i increase) has been characterized to code mechanosensitive calcium-permeable channels as osmosensors in plants [ 9 , 10 , 11 ]. In addition, there are multiple OSCA gene copies in the genome, and the OSCA gene family has been identified in many species, such as soybean, barley, wheat, and rice [ 12 , 13 , 14 , 15 , 16 ]. In Arabidopsis , the OSCA1 protein has been shown as a calcium-permeable channel sensitive to hyperosmotic conditions.…”

Section: Introductionmentioning

confidence: 99%

Long Noncoding RNAs in Response to Hyperosmolarity Stress, but Not Salt Stress, Were Mainly Enriched in the Rice Roots

Pang,

Zheng,

Min

et al. 2024

IJMS

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Due to their immobility and possession of underground parts, plants have evolved various mechanisms to endure and adapt to abiotic stresses such as extreme temperatures, drought, and salinity. However, the contribution of long noncoding RNAs (lncRNAs) to different abiotic stresses and distinct rice seedling parts remains largely uncharacterized beyond the protein-coding gene (PCG) layer. Using transcriptomics and bioinformatics methods, we systematically identified lncRNAs and characterized their expression patterns in the roots and shoots of wild type (WT) and ososca1.1 (reduced hyperosmolality-induced [Ca2+]i increase in rice) seedlings under hyperosmolarity and salt stresses. Here, 2937 candidate lncRNAs were identified in rice seedlings, with intergenic lncRNAs representing the largest category. Although the detectable sequence conservation of lncRNAs was low, we observed that lncRNAs had more orthologs within the Oryza. By comparing WT and ososca1.1, the transcription level of OsOSCA1.1-related lncRNAs in roots was greatly enhanced in the face of hyperosmolality stress. Regarding regulation mode, the co-expression network revealed connections between trans-regulated lncRNAs and their target PCGs related to OsOSCA1.1 and its mediation of hyperosmolality stress sensing. Interestingly, compared to PCGs, the expression of lncRNAs in roots was more sensitive to hyperosmolarity stress than to salt stress. Furthermore, OsOSCA1.1-related hyperosmolarity stress-responsive lncRNAs were enriched in roots, and their potential cis-regulated genes were associated with transcriptional regulation and signaling transduction. Not to be ignored, we identified a motif-conserved and hyperosmolarity stress-activated lncRNA gene (OSlncRNA), speculating on its origin and evolutionary history in Oryza. In summary, we provide a global perspective and a lncRNA resource to understand hyperosmolality stress sensing in rice roots, which helps to decode the complex molecular networks involved in plant sensing and adaptation to stressful environments.

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Insights into membrane lipids modification in barley leaves as an adaptation mechanism to cold stress

Zhao,

Li,

et al. 2024

Plant Growth Regul

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Genomewide identification and analysis of the OSCA gene family in barley (Hordeum vulgare L.)

Cited by 3 publications

References 50 publications

Genome-wide investigation and expression analysis of OSCA gene family in response to abiotic stress in alfalfa

Genome-wide investigation and expression analysis of OSCA gene family in response to abiotic stress in alfalfa

Long Noncoding RNAs in Response to Hyperosmolarity Stress, but Not Salt Stress, Were Mainly Enriched in the Rice Roots

Insights into membrane lipids modification in barley leaves as an adaptation mechanism to cold stress

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