Genomewide Analysis of Divergence of Antibiotic Resistance Determinants in Closely Related Isolates of
            <i>Acinetobacter baumannii</i>

Adams, Mark D.; Chan, E. Ricky; Molyneaux, Neil; Bonomo, Robert A.

doi:10.1128/aac.00057-10

Cited by 102 publications

(98 citation statements)

References 41 publications

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“…In support of these observations, Adams et al (2) similarly found variability in resistance genes associated with insertion sequences, plasmids, and the AbaR chromosomal resistance gene island among closely related clinical isolates of A. baumannii. Some of the differences between isolates were of a more general nature, such as the association of cusS with representatives of international clone I (and III) but not with those of international clone II.…”

Section: Discussionsupporting

confidence: 51%

“…In addition, a number of "AbaR"-type resistance islands, inserted in an ATPase gene (here called comM) and carrying genes associated with resistance to antibiotics and heavy metals, have been described previously (1,2,11,16,17,20,21). The latter show diversity, with that described in an isolate of international clone II (AbaR2 in ACICU) being considerably smaller than that found in isolates of international clone I; among the latter, a number of different islands have already been described.…”

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confidence: 97%

“…The presence of different acquired carbapenem-hydrolyzing oxacillinase (CHDL) genes in isolates of the international clonal lineages (and other isolates) is well documented, with bla OXA-58-like being particularly associated with isolates from Greece and Italy (10,24), bla OXA-40-like being associated with isolates from Spain and Portugal (5,6,22,23), and bla OXA-23-like being associated with those from the United Kingdom, Asia, and South America (3,13,19,32). The study by Adams et al (2) demonstrated that the resistance genotype of closely related isolates was variable, with each isolate examined having a unique genetic repertoire. Our aim was to seek genes of the accessory genome in numerous isolates, of known international clonal lineage and PFGE and VNTR type and various degrees of relatedness, to see if such analysis would be helpful in epidemiological studies.…”

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confidence: 99%

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Use of the Accessory Genome for Characterization and Typing of Acinetobacter baumannii

Turton¹,

Baddal²,

Perry³

2011

J Clin Microbiol

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Outbreak strains of Acinetobacter baumannii are highly clonal, and cross-infection investigations can be difficult. We sought targets based on AbaR resistance islands and on other genes found in some, but not all, sequenced isolates of A. baumannii among a set of clinical isolates (n ‫؍‬ 70) that included multiple representatives of a number of pulsed-field gel electrophoresis (PFGE)-defined types. These included representatives that varied in their profiles at two variable-number tandem repeat (VNTR) loci, which can provide discrimination within a PFGE cluster. Detection, or not, of each element sought provided some degree of discrimination among the set, with the presence or absence of genes coding for a phage terminase (ACICU_02185), a sialic acid synthase (ACICU_00080), a polysaccharide biosynthesis protein (AB57_0094), aphA1, bla TEM , and integron-associated orfX (Kyoto Encyclopedia of Genes and Genomes [KEGG] no. K03830) proving the most helpful in discriminating between closely related isolates in our panel. The results support VNTR data in describing distinct populations of highly similar isolates. Such analysis, in combination with other typing methods, can inform epidemiological investigations and provide additional characterization of isolates. Most genotypes carrying bla OXA-23-like were PCR positive for a yeeA-bla OXA-23 fragment found in an AbaR4-type island, suggesting that this is widespread.

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Section: Discussionsupporting

confidence: 51%

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confidence: 97%

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confidence: 99%

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Use of the Accessory Genome for Characterization and Typing of Acinetobacter baumannii

Turton¹,

Baddal²,

Perry³

2011

J Clin Microbiol

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“…Patient 4, who had a prolonged stay in ICU A during the outbreak, harbored isolates both with and without this plasmid. Although plasmids have been shown to be important in the dissemination of antibiotic resistance during outbreaks (80,81), they are of limited value for defining transmission pathways, because they are frequently gained and lost by closely related circulating strains (74, …”

Section: Resultsmentioning

confidence: 99%

Utility of Whole-Genome Sequencing in Characterizing Acinetobacter Epidemiology and Analyzing Hospital Outbreaks

2016

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bAcinetobacter baumannii frequently causes nosocomial infections and outbreaks. Whole-genome sequencing (WGS) is a promising technique for strain typing and outbreak investigations. We compared the performance of conventional methods with WGS for strain typing clinical Acinetobacter isolates and analyzing a carbapenem-resistant A. baumannii (CRAB) outbreak. We performed two band-based typing techniques (pulsed-field gel electrophoresis and repetitive extragenic palindromic-PCR), multilocus sequence type (MLST) analysis, and WGS on 148 Acinetobacter calcoaceticus-A. baumannii complex bloodstream isolates collected from a single hospital from 2005 to 2012. Phylogenetic trees inferred from core-genome single nucleotide polymorphisms (SNPs) confirmed three Acinetobacter species within this collection. Four major A. baumannii clonal lineages (as defined by MLST) circulated during the study, three of which are globally distributed and one of which is novel. WGS indicated that a threshold of 2,500 core SNPs accurately distinguished A. baumannii isolates from different clonal lineages. The bandbased techniques performed poorly in assigning isolates to clonal lineages and exhibited little agreement with sequence-based techniques. After applying WGS to a CRAB outbreak that occurred during the study, we identified a threshold of 2.5 core SNPs that distinguished nonoutbreak from outbreak strains. WGS was more discriminatory than the band-based techniques and was used to construct a more accurate transmission map that resolved many of the plausible transmission routes suggested by epidemiologic links. Our study demonstrates that WGS is superior to conventional techniques for A. baumannii strain typing and outbreak analysis. These findings support the incorporation of WGS into health care infection prevention efforts.A n important role of clinical microbiology is to identify relationships between bacterial isolates. At a broad level, phenotypic and genotypic tests are used to categorize bacterial isolates into the same or different species. Within a bacterial species, techniques are used to group isolates into clonal lineages, which are groups of closely related bacteria that share a recent common ancestor but have spread regionally or globally. At a more local level, infection control practitioners must determine whether a group of isolates constitutes a hospital outbreak by ascertaining whether the isolates have a degree of similarity consistent with a common source within the hospital. Once isolates belonging to a hospital outbreak have been identified, similarities and differences between these isolates can be exploited to generate a transmission map to aid in finding the source of the outbreak and in disrupting ongoing pathways of transmission. For some groups of bacteria, such as Acinetobacter, discernment at each level has medically important consequences and therefore must be accomplished by hospital-associated clinical microbiology laboratories.Within the Acinetobacter genus, the Acinetobacter calcoaceticusAcinetobac...

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“…Mobile elements and resistance determinants associated to antibiotic resistance have been well studied for A. baumannii [10][11][12], in contrast there are few reports of these elements in A. junii [13,14].…”

Section: Introductionmentioning

confidence: 99%

Bacteremia caused by an Acinetobacter junii strain harboring class 1 integron and diverse DNA mobile elements

Traglia

Almuzara²,

Vilacoba

et al. 2014

J Infect Dev Ctries

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Introduction: Infections caused by Acinetobacter junii are rarely reported. However, some outbreaks of septicemia in neonates and pediatric oncology patients, as well as meningitis, peritonitis, and ocular infection have been described. Since it is highly infrequent to find the molecular characterization of A. junii strains in literature, in this study we described the molecular characterization of A. junii isolates recovered from blood samples of a renal transplant patient. Methodology: The case was defined as a catheter-related bacteremia caused by A. junii. The patient responded favorably after catheter removal and treatment with ciprofloxacin. Results and Conclusion: The complete molecular characterization of the isolate showed that it harbored a class 1 integron and diverse DNA mobile elements. This explains its genomic plasticity for acquiring antimicrobial resistance determinants and for adapting to a nosocomial niche.

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Genomewide Analysis of Divergence of Antibiotic Resistance Determinants in Closely Related Isolates of Acinetobacter baumannii

Cited by 102 publications

References 41 publications

Use of the Accessory Genome for Characterization and Typing of Acinetobacter baumannii

Use of the Accessory Genome for Characterization and Typing of Acinetobacter baumannii

Utility of Whole-Genome Sequencing in Characterizing Acinetobacter Epidemiology and Analyzing Hospital Outbreaks

Bacteremia caused by an Acinetobacter junii strain harboring class 1 integron and diverse DNA mobile elements

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