Genome-wide Survey of the Dehydrin Genes in Bread Wheat (Triticum Aestivum L.) and its Relatives: Identification, Evolution and Expression Profiling Under Various Abiotic Stresses

Abstract

Background: Bread wheat (Triticum aestivum) is an important and fundamental cereal worldwide. With increasingly severe environmental stress, it is very important to mine stress-resistant genes for wheat breeding. Dehydrin (DHN) genes are primary candidates because they are involved in the response to many stressors. Results: Here, a genome-wide analysis of this gene family was performed on the genomes of wheat and its three relatives. A total of 55 DHN genes in Triticum aestivum, 31 in Triticum dicoccoides, 15 in Triticum urartu, and 16 in Aegilops tauschii were identified. The phylogenetic, synteny, sequence and protein structure analyses showed that the DHN genes were divided into five groups, Genes in the same group share similar conserved motifs, protein structures, and potential functions. The tandem TaDHN genes responded strongly to drought, cold and high salinity stresses, while the non-tandem genes were responded weakly to all stress conditions. Further, multiple DHN proteins cooperation maybe an important way to prevent plants from abiotic stress according to the interaction network analysis. Conclusions: Conserved, duplicated DHN genes may have played an important role in the adaptation of wheat to a variety of conditions, hence, contributing to the distribution of bread wheat as a global staple food. This research illuminates the contributions of DHN genes to abiotic stresses in Triticeae species and offers helpful information for further functional study of DHN genes in these crops.

Ultrasound, as a hypomethylating agent, remodels DNA methylation and alters mRNA transcription in winter wheat (Triticum aestivum L.) seedlings

“…When the Aegilops tauschii (Tausch's goatgrass) genome was resequenced and reannotated, and then compared with the T. aestivum genome, these genes were identified as orthologous genes (Zhou et al 2021), which were similar LFC values (−4.22 and −4.23) detected in our present study (Table 1). TraesCS6A02G350300 gene encodes dehydrin (DHN) protein ( TaDHN10‐A gene) (Hao et al 2022). The taDHN10‐A gene was upregulated with high TPM (transcripts per kilobase million) values under drought and cold stresses (Wang et al 2014, Hao et al 2022).…”

“…The taDHN10‐A gene was upregulated with high TPM (transcripts per kilobase million) values under drought and cold stresses (Wang et al 2014, Hao et al 2022). DHN genes respond to biotic and abiotic stress on a high scale of gene expression intensity (up‐, or downregulation), depending on the stress type (Hao et al 2022). The DHN gene was reported earlier to be downregulated under ultrasonication treatment (Oda et al 2021).…”

“…TraesCS6A02G350300 gene encodes dehydrin (DHN) protein ( TaDHN10‐A gene) (Hao et al 2022). The taDHN10‐A gene was upregulated with high TPM (transcripts per kilobase million) values under drought and cold stresses (Wang et al 2014, Hao et al 2022). DHN genes respond to biotic and abiotic stress on a high scale of gene expression intensity (up‐, or downregulation), depending on the stress type (Hao et al 2022).…”