Genome-wide study of salivary miRNAs identifies miR-423-5p as promising diagnostic and prognostic biomarker in oral squamous cell carcinoma

Romani, Chiara; Salviato, Elisa; Paderno, Alberto; Zanotti, Laura; Ravaggi, Antonella; Deganello, Alberto; Berretti, Giulia; Gualtieri, Tommaso; Marchini, Sergio; D’Incalci, Maurizio; Mattavelli, Davide; Piazza, Cesare; Bossi, Paolo; Romualdi, Chiara; Nicolai, Piero; Bignotti, Eliana

doi:10.7150/thno.45157

Cited by 40 publications

(41 citation statements)

References 45 publications

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“…In the past decade, great progress has been made in the research and treatment of LSCC [13,14]. However, treatment resistance increases the recurrence rate of LSCC and patients who undergo total laryngectomy have a poor quality of life [15].…”

Section: Discussionmentioning

confidence: 99%

Plasma cell-free circRNAs panel act as fingerprint predicts the occurrence of laryngeal squamous cell carcinoma

Han

Lin

Dong

2021

Aging

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Background: Circular RNAs (circRNAs) have recently emerged as a new class of RNAs, highly enriched in the human tissues and very stable within cells, exosomes and body uids. In this study, we aimed to screen the plasma cell-free derived circRNAs in laryngeal squamous cell carcinoma (LSCC) and investigate whether these circRNAs could predicted LSCC as potential biomarkers.Methods: The circRNA microarray was employed with three samples in each group to screen the dysregulated circRNAs isolated from plasma samples. The top 20 circRNAs were rst selected as candidates with the upregulated level in the plasma of LSCC.Results: Further validation found that only circ_0019201, circ_0011773 and circ_0122790 was consistent with training set. The ROC curve also revealed a high diagnostic ability an area under ROC curve value (AUC) for single circRNA and combined. The AUC for circ_0019201, circ_0011773 and circ_0122790 and the combined was 0.933, 0.908, 0.965 and 0.990 in training set. For the validation set, the AUC was 0.766, 0.864, 0.908 and 0.951. The three circRNAs were further investigated with stable expression in human plasma samples.Conclusions: The plasma derived circ_0019201, circ_0011773 and circ_0122790 might be the potential biomarker for predicting the LSCC.

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Section: Discussionmentioning

confidence: 99%

Plasma cell-free circRNAs panel act as fingerprint predicts the occurrence of laryngeal squamous cell carcinoma

Han

Lin

Dong

2021

Aging

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“…The cell-free RNA containing supernatant was aliquoted (500 µL) and stored at −80 °C until further RNA extraction. As a validation set, the data of OSCC patients at Medical Oncology, ASST Spedali Civili di Brescia, Brescia, Italy (SCUB cohort), previously described by Romani and colleagues [ 24 ], were kindly provided to the authors. The dataset included microarray analysis performed on 50 OSCC saliva samples and 42 samples from healthy subjects.…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, their stability and tissue- and disease-specific expression pattern in human peripheral blood and bodily fluids provide miRNAs with ideal biomarker properties for early diagnosis and prognosis of cancer [ 18 ]. Recent studies have suggested that thousands of miRNAs are present in salivary exosomes and they can be used as minimally invasive diagnostic biomarkers to detect OSCC and other malignancies [ 19 , 20 , 21 , 22 , 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Salivary miR-30c-5p as Potential Biomarker for Detection of Oral Squamous Cell Carcinoma

Mehterov

Vladimirov

Sacconi³

et al. 2021

Biomedicines

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The levels of different classes of extracellular RNAs (exRNAs) remain stable in bodily fluids. The detection of either enriched or depleted specific subsets of salivary microRNAs (miRNAs) has the potential to serve as a non-invasive approach for biomarker development. Thus, salivary miRNAs have emerged as a promising molecular tool for early diagnosis and screening of oral squamous cell carcinoma (OSCC). Total RNA was extracted from saliva supernatant of 33 OSCC patients and 12 controls (discovery set), and the differential expression of 8 cancer-related miRNAs was detected by TaqMan assay. Among the screened miRNAs, miR-30c-5p (p < 0.04) was significantly decreased in OSCC saliva. The same transcriptional behavior of miR30c-5p was observed in an additional validation set. miR-30c-5p showed a significant statistical difference between cases and controls with areas under the curve (AUC) of 0.82 (95% CI: 0.71–0.89). The sensitivity and the specificity of miR-30c-5p were 86% and 74%, respectively. The target identification analysis revealed enrichment of miR-30c-5p targets in p53 and Wnt signaling pathways in OSCC. Additionally, the miR-30c-5p targets had clinical significance related to overall survival. In conclusion, these findings show that downregulated miR-30c-5p has the potential to serve as a novel, non-invasive biomarker for early OSCC detection.

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“…High levels of miR-21, miR-345 and miR-181b and low levels of miR 142-5 are associated with malignant progression of OPMD; final stages of carcinogenesis may also involve changes in miR-196a and miR-206. Various reports support the role of these miRs in carcinogenesis due to the involvement in the regulation of target genes, such as TPM1, PTEN, and bcl-2 in the regulation of immune system [88][89][90][91][92][93]. Regarding epigenetic modifications, in some series, 40% of cases had hypermethylation of p16 inhibitor of cyclin-dependent kinase4A, and almost all of these cases evolved to squamous cell carcinoma [94].…”

Section: Molecular Alterationsmentioning

confidence: 99%

Overview of Oral Potentially Malignant Disorders: From Risk Factors to Specific Therapies

Lorini

Bescós‐Atín

Thavaraj

et al. 2021

Cancers

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Oral squamous cell carcinoma (OSCC) is a very aggressive cancer, representing one of the most common malignancies worldwide. Oral potentially malignant disorders (OPMDs) regroup a variegate set of different histological lesions, characterized by the potential capacity to transform in OSCC. Most of the risk factors associated with OSCC are present also in OPMDs’ development; however, the molecular mechanisms and steps of malignant transformation are still unknown. Treatment of OSCC, including surgery, systemic therapy and radiotherapy (alone or in combination), has suffered a dramatic change in last years, especially with the introduction of immunotherapy. However, most cases are diagnosed during the advanced stage of the disease, decreasing drastically the survival rate of the patients. Hence, early diagnosis of premalignant conditions (OPMDs) is a priority in oral cancer, as well as a massive education about risk factors, the understanding of mechanisms involved in malignant progression and the development of specific and more efficient therapies. The aim of this article is to review epidemiological, clinical, morphological and molecular features of OPMDs, with the purpose to lay the foundation for an exhaustive comprehension of these lesions and their ability of malignant transformation and for the development of more effective and personalized treatments.

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Genome-wide study of salivary miRNAs identifies miR-423-5p as promising diagnostic and prognostic biomarker in oral squamous cell carcinoma

Cited by 40 publications

References 45 publications

Plasma cell-free circRNAs panel act as fingerprint predicts the occurrence of laryngeal squamous cell carcinoma

Plasma cell-free circRNAs panel act as fingerprint predicts the occurrence of laryngeal squamous cell carcinoma

Salivary miR-30c-5p as Potential Biomarker for Detection of Oral Squamous Cell Carcinoma

Overview of Oral Potentially Malignant Disorders: From Risk Factors to Specific Therapies

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