Genome-Wide Screening of Genes Showing Altered Expression in Liver Metastases of Human Colorectal Cancers by cDNA Microarray

Yanagawa, Rempei; Furukawa, Yoichi; Tsunoda, Tatsuhiko; Kitahara, Osamu; Kameyama, Masao; Murata, Kohei; Ishikawa, Osamu; Nakamura, Yusuke

doi:10.1038/sj.neo.7900185

Cited by 86 publications

(60 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, the studies have been mainly conducted on primary tumours and only very few data are available on metastases (Yanagawa et al, 2001;Koehler et al, 2004). This study first reports the gene expression profiling of liver metastases from colorectal cancer.…”

Section: Discussionmentioning

confidence: 99%

“…normal mucosa, and also to evaluating the potential for metastases developing (Alon et al, 1999;Notterman et al, 2001;Yanagawa et al, 2001;Bertucci et al, 2004;Koehler et al, 2004;Li et al, 2004;Wang et al, 2004;Eschrich et al, 2005). Moreover, the studies have been mainly conducted on primary tumours and only very few data are available on metastases (Yanagawa et al, 2001;Koehler et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Gene expression profiling of liver metastases from colorectal cancer as potential basis for treatment choice

et al. 2008

View full text Add to dashboard Cite

At present no reports on gene expression profiling of liver metastases from colorectal cancer are available. We identified two different signatures using Affymetrix platform: epidermal growth factor receptor pathway was upregulated in metachronous lesions, whereas the pathway mainly related to angiogenesis was in synchronous lesions. Synchronous or metachronous liver metastases could be treated differently on the basis of different molecular pathways.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Gene expression profiling of liver metastases from colorectal cancer as potential basis for treatment choice

et al. 2008

View full text Add to dashboard Cite

show abstract

“…Gene expression analysis of colorectal biopsies using high density oligonucleotide microarrays could help detect such gene expression profiles that would establish the basis for new molecular diagnostic methods, and efforts have been made in this direction. In order to understand the gene expression background of CRC progression and metastasis development, many previous studies have primarily focused on the pair-wise comparisons of discriminative mRNA expression signatures between CRC and normal colorectal mucosa (NOR) samples, between CRA and NOR samples, as well as between the early and advanced stages of CRC (15)(16)(17)(18)(19)(20)(21)(22)(23)(24). Results from various studies have demonstrated that CRC and/or CRA have widespread genetic alterations compared to NOR (25)(26)(27).…”

Section: Identification Of An Intermediate Signature That Marks the Imentioning

confidence: 99%

Identification of an intermediate signature that marks the initial phases of the colorectal adenoma-carcinoma transition

Tang

Guo²,

Chao³

et al. 2010

Int J Mol Med

View full text Add to dashboard Cite

Abstract. The colorectal adenoma-carcinoma sequence describes the stepwise progression from normal to dysplastic epithelium and then to carcinoma. Only a small proportion of colorectal adenomas (CRAs) progress to colorectal carcinomas (CRCs). Endoscopic intervention is currently being used on patients with high grade dysplasia CRAs, with diameters of >1 cm, or villous components of >25% who are at higher risk than other CRA sufferers. During the process, biopsy samples are taken for conventional histological diagnosis, but poor pathomorphological sensitivity and specificity greatly limit the diagnostic accuracy. Unfortunately, there are no reliable molecular criteria available that can predict the potential development of CRA to CRC. Gene expression profiles of normal colorectal mucosa (NOR), CRA and different Dukes' stages of CRC biopsy specimens, which represent the gradual progress of the CRA to CRC sequence, were determined by Affymetrix technology. Representative regulated genes were further analyzed by quantitative real-time PCR (qRT-PCR) and immunohistochemistry (IHC). Intersectional analyses of discriminative expression signatures of CRC vs. CRA and CRA vs. NOR allowed the identification of an intermediate signature of 463 probe sets (psets) that mark the NOR➝ CRA➝CRC progression. This signature represents a reservoir of candidate markers for the early diagnosis of higher-risk CRA, thus allowing for timely therapeutic intervention and more selective treatment. A further 279 CRC-specific psets pointing to the malignant transition from CRA to CRC were identified and these could represent potential therapeutic targets for CRC. The reliability of the results was further confirmed by qRT-PCR and IHC analyses of the 4-gene sets randomly selected from the 463 psets.

show abstract

“…In addition to the mutations to these important oncogenes, colon cancer initiation and progression are accompanied by changes in gene expression. Several microarray studies have been undertaken to more fully elucidate the changes in gene expression that occur during CRC initiation, progression and metastasis (3)(4)(5)(6).…”

Section: Introductionmentioning

confidence: 99%

Tissue-dependent and -independent gene expression changes in metastatic colon cancer

Gmeiner

Hellmann²,

Shen³

2008

Oncol Rep

View full text Add to dashboard Cite

Abstract. The goal of this study was to identify systematic alterations in key cell signaling and metabolic pathways that occur during colon cancer carcinogenesis and metastasis. Understanding gene expression changes in the context of specific pathways may increase our understanding of carcinogenesis and help guide treatment. Ten cases, with matched controls, were profiled for expression of >18,000 human transcripts using Affymetrix U133A chips. Data were filtered using GeneSifter™. Gene expression levels for primary colon samples were compared to a normal colon while metastatic tissues were compared to the primary colon. Differentially regulated genes were associated using the Kyoto encyclopedia of genes and genome pathways to identify cell signaling and metabolic pathways altered during carcinogenesis and metastasis. Primary colon samples displayed high positive z-scores (indicating a gene ontology term that occurs more frequently than expected) for genes involved in Wnt-signaling (4.11), nitrogen metabolism (7.30) and inositol phosphate metabolism (2.47). Expression level changes for individual genes in each cluster were statistically significant (e.g. p=0.017 for cyclin D1 in the Wnt-signaling cluster). Metastatic tissue from the liver and omentum, but not the lung, displayed a decreased expression of genes important for oxidative phosphorylation. The metastatic tissue from all sites displayed a substantially decreased expression for genes involved in butanoate and propanoate metabolism and valine, leucine and isoleucine degradation. Our results demonstrate that systematic changes in gene expression occur for proteins involved in key cell signaling and metabolic pathways during the course of carcinogenesis and metastasis. These expression level changes complement the spectrum of mutations that characterize the progression of colorectal cancer.

show abstract

Genome-Wide Screening of Genes Showing Altered Expression in Liver Metastases of Human Colorectal Cancers by cDNA Microarray

Cited by 86 publications

References 10 publications

Gene expression profiling of liver metastases from colorectal cancer as potential basis for treatment choice

Gene expression profiling of liver metastases from colorectal cancer as potential basis for treatment choice

Identification of an intermediate signature that marks the initial phases of the colorectal adenoma-carcinoma transition

Tissue-dependent and -independent gene expression changes in metastatic colon cancer

Contact Info

Product

Resources

About