Genome-wide profiling of sperm DNA methylation in relation to buffalo (Bubalus bubalis) bull fertility

Verma, Arpana; Rajput, Sandeep; De, Sachinandan; Kumar, Rakesh; Chakravarty, A. K.; Datta, Tirtha Kumar

doi:10.1016/j.theriogenology.2014.06.012

Cited by 54 publications

(47 citation statements)

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“…CCNB2 was identified as significantly associated with developmental competence of bovine oocytes [25]. PDZRN4 is associated with sperm motility of Holstein-Friesian cattle and EEFSEC is related to buffalo bull fertility [26, 27]. Health traits: TMEM154 can reduce lentivirus susceptibility in sheep [28] and GWAS indicate this gene to be associated with susceptibility to and control of ovine lentivirus [29].…”

Section: Resultsmentioning

confidence: 99%

“…For reproduction traits, we found no major genes controlling reproduction prolificacy, such as GDF9 and BMPR1B ; however, we found some genes which can influence development of the oocyte and sperm. For example, EIF3F , CCNB2 and SLC8A3 affect oocyte development [24, 25, 43] and PDZRN4 and EEFSEC affect sperm [26, 27]. For meat traits, ALDOA , STK32B and FAM190A are related to marbling in cattle[19, 20, 58].…”

Section: Discussionmentioning

confidence: 99%

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Genome-Wide Specific Selection in Three Domestic Sheep Breeds

et al. 2015

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BackgroundCommercial sheep raised for mutton grow faster than traditional Chinese sheep breeds. Here, we aimed to evaluate genetic selection among three different types of sheep breed: two well-known commercial mutton breeds and one indigenous Chinese breed.ResultsWe first combined locus-specific branch lengths and di statistical methods to detect candidate regions targeted by selection in the three different populations. The results showed that the genetic distances reached at least medium divergence for each pairwise combination. We found these two methods were highly correlated, and identified many growth-related candidate genes undergoing artificial selection. For production traits, APOBR and FTO are associated with body mass index. For meat traits, ALDOA, STK32B and FAM190A are related to marbling. For reproduction traits, CCNB2 and SLC8A3 affect oocyte development. We also found two well-known genes, GHR (which affects meat production and quality) and EDAR (associated with hair thickness) were associated with German mutton merino sheep. Furthermore, four genes (POL, RPL7, MSL1 and SHISA9) were associated with pre-weaning gain in our previous genome-wide association study.ConclusionsOur results indicated that combine locus-specific branch lengths and di statistical approaches can reduce the searching ranges for specific selection. And we got many credible candidate genes which not only confirm the results of previous reports, but also provide a suite of novel candidate genes in defined breeds to guide hybridization breeding.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genome-Wide Specific Selection in Three Domestic Sheep Breeds

et al. 2015

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“…Epigenetics is the study of genomic structural modifications that affect gene expression without altering the underlying nucleotide sequence (1)(2)(3). Epigenetic mechanisms involved in the regulation of gene expression include the regulation of non-coding RNA, chromatin remodeling, DNA methylation and histone modifications (4,5).…”

Section: Introductionmentioning

confidence: 99%

“…Epigenetic mechanisms involved in the regulation of gene expression include the regulation of non-coding RNA, chromatin remodeling, DNA methylation and histone modifications (4,5). Of these mechanisms, DNA methylation has been implicated in numerous biological functions, such as the development of spermatozoa and early embryos, and the repression of endogenous retrotransposons, while it also has a wide range of effects in gene expression (2,3,6). The dysregulation of DNA methylation has previously been associated with various human disorders, and has been shown to increase the risk of fertilization failure, dysfunction in embryogenesis, perinatal mortality, congenital abnormalities, preterm birth and low birth weight (7)(8)(9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

DNA methylation in spermatogenesis and male infertility

Cui¹,

Xuan

Wu³

et al. 2016

Experimental and Therapeutic Medicine

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Abstract. Infertility is a significant problem for human reproduction, with males and females equally affected. However, the molecular mechanisms underlying male infertility remain unclear. Spermatogenesis is a highly complex process involving mitotic cell division, meiosis cell division and spermiogenesis; during this period, unique and extensive chromatin and epigenetic modifications occur to bring about specific epigenetic profiles in spermatozoa. It has recently been suggested that the dysregulation of epigenetic modifications, in particular the methylation of sperm genomic DNA, may serve an important role in the development of numerous diseases. The present study is a comprehensive review on the topic of male infertility, aiming to elucidate the association between sperm genomic DNA methylation and poor semen quality in male infertility. In addition, the current status of the genetic and epigenetic determinants of spermatogenesis in humans is discussed. Contents1. Introduction 2. DNA methylation 3. DNA methylation and spermatogenesis 4. DNA methylation and genomic imprinting 5. DNA methylation and male infertility 6. Conclusion IntroductionEpigenetics is the study of genomic structural modifications that affect gene expression without altering the underlying nucleotide sequence (1-3). Epigenetic mechanisms involved in the regulation of gene expression include the regulation of non-coding RNA, chromatin remodeling, DNA methylation and histone modifications (4,5). Of these mechanisms, DNA methylation has been implicated in numerous biological functions, such as the development of spermatozoa and early embryos, and the repression of endogenous retrotransposons, while it also has a wide range of effects in gene expression (2,3,6). The dysregulation of DNA methylation has previously been associated with various human disorders, and has been shown to increase the risk of fertilization failure, dysfunction in embryogenesis, perinatal mortality, congenital abnormalities, preterm birth and low birth weight (7-11). The present review assesses the significance of DNA methylation in spermatogenesis in order to elucidate the association between the dysregulation of DNA methylation and male infertility. This may provide a basis for the prevention and treatment of male infertility, as well as permit the evaluation of the epigenetic quality of sperm in order to reduce the risk of epigenetic diseases in cases where conception is performed by assisted reproductive technology (ART). DNA methylationEpigenetic mechanisms are critical regulators of gene expression during spermatogenesis that may influence male fertility (12-14). Cytosine, a key DNA base, is methylated at the position, typically in the context of CpG dinucleotides. The methylation of constitutive heterochromatic and promoter regions is generally associated with reduced gene transcription (Fig. 1A) (15-18). Therefore, DNA methylation is a type of epigenetic modification that can effectively promote gene silencing (Fig. 1B). The methyl group for this chemical modif...

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“…In addition, there is an interesting report that implies that bull spermatozoa with a morphologically abnormal head are less capable of swimming up to the ampulla of the oviduct than morphologically normal spermatozoa, but that the spermatozoa with vacuoles in the head can reach oocytes in vivo, as well as morphologically normal spermatozoa 32. For examinations of the sperm genome for the prediction of bull AI fertility, the sperm chromatin structure assay with acridine orange staining,33, 34 evaluation of DNA damage by the terminal deoxynucleotidyl transferase‐mediated dUTP nick end labeling (TUNEL) assay,35 and sperm DNA methylation analyses36 are available for cattle. Moreover, one of the well‐working assays is the superovulation/AI embryo‐collection test, which was designed for Japanese Black cattle by Fukushima et al.…”

Section: Introductionmentioning

confidence: 99%

Protein biomarkers for male artificial insemination subfertility in bovine spermatozoa

Harayama

Minami

Kishida

et al. 2017

Reprod Medicine & Biology

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BackgroundAlthough artificial insemination (AI) technique is an established biotechnology for bovine reproduction, the results of AI (conception rates) have a tendency to decline gradually. To our annoyance, moreover, AI‐subfertile bulls have been occasionally found in the AI centers. To resolve these serious problems, it is necessary to control the sperm quality more strictly by the examinations of sperm molecules.MethodsWe reviewed a number of recent articles regarding potentials of bovine sperm proteins as the biomarkers for bull AI‐subfertility and also showed our unpublished supplemental data on the bull AI‐subfertility associated proteins.Main findingsBull AI‐subfertility is caused by the deficiency or dysfunctions of various molecules including regulatory proteins of ATP synthesis, acrosomal proteins, nuclear proteins, capacitation‐related proteins and seminal plasma proteins.ConclusionIn order to control the bovine sperm quality more strictly by the molecular examinations, it is necessary to select suitable sperm protein biomarkers for the male reproductive problems which happen in the AI centers.

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Genome-wide profiling of sperm DNA methylation in relation to buffalo (Bubalus bubalis) bull fertility

Cited by 54 publications

References 55 publications

Genome-Wide Specific Selection in Three Domestic Sheep Breeds

Genome-Wide Specific Selection in Three Domestic Sheep Breeds

DNA methylation in spermatogenesis and male infertility

Protein biomarkers for male artificial insemination subfertility in bovine spermatozoa

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