Genome-wide post-transcriptional regulation of bovine mammary gland response to Streptococcus uberis

Tabashiri, Raana; Sharifi, Seyed Davood; Pakdel, A.; Bakhtiarizadeh, Mohammad Reza; Pakdel, Mohammad Hosein; Tahmasebi, Ahmad; Hercus, Colin

doi:10.1007/s13353-022-00722-y

Cited by 5 publications

(5 citation statements)

References 88 publications

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“…miRNAs are small non-coding RNAs (~ 22 nucleotides) that post-transcriptionally regulate gene expression by binding to target mRNAs 27 . Several studies have reported differentially expressed (DE) miRNAs in response to S. uberis infection, such as bta-miR-200c , bta-miR-182 , bta-miR-30a-5p , bta-miR-146b , and bta-miR-125a , which are key amplifiers of monocyte inflammatory response networks 7 , 28 . LncRNAs, on the other hand, are longer non-coding RNAs (> 200 bp) that regulate gene expression through diverse mechanisms at the transcriptional, post-transcriptional, and translational levels.…”

Section: Introductionmentioning

confidence: 99%

Integrated analysis of inflammatory mRNAs, miRNAs, and lncRNAs elucidates the molecular interactome behind bovine mastitis

Hasankhani,

Bakherad,

Bahrami

et al. 2023

Sci Rep

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Mastitis is known as intramammary inflammation, which has a multifactorial complex phenotype. However, the underlying molecular pathogenesis of mastitis remains poorly understood. In this study, we utilized a combination of RNA-seq and miRNA-seq techniques, along with computational systems biology approaches, to gain a deeper understanding of the molecular interactome involved in mastitis. We retrieved and processed one hundred transcriptomic libraries, consisting of 50 RNA-seq and 50 matched miRNA-seq data, obtained from milk-isolated monocytes of Holstein–Friesian cows, both infected with Streptococcus uberis and non-infected controls. Using the weighted gene co-expression network analysis (WGCNA) approach, we constructed co-expressed RNA-seq-based and miRNA-seq-based modules separately. Module-trait relationship analysis was then performed on the RNA-seq-based modules to identify highly-correlated modules associated with clinical traits of mastitis. Functional enrichment analysis was conducted to understand the functional behavior of these modules. Additionally, we assigned the RNA-seq-based modules to the miRNA-seq-based modules and constructed an integrated regulatory network based on the modules of interest. To enhance the reliability of our findings, we conducted further analyses, including hub RNA detection, protein–protein interaction (PPI) network construction, screening of hub-hub RNAs, and target prediction analysis on the detected modules. We identified a total of 17 RNA-seq-based modules and 3 miRNA-seq-based modules. Among the significant highly-correlated RNA-seq-based modules, six modules showed strong associations with clinical characteristics of mastitis. Functional enrichment analysis revealed that the turquoise module was directly related to inflammation persistence and mastitis development. Furthermore, module assignment analysis demonstrated that the blue miRNA-seq-based module post-transcriptionally regulates the turquoise RNA-seq-based module. We also identified a set of different RNAs, including hub-hub genes, hub-hub TFs (transcription factors), hub-hub lncRNAs (long non-coding RNAs), and hub miRNAs within the modules of interest, indicating their central role in the molecular interactome underlying the pathogenic mechanisms of S. uberis infection. This study provides a comprehensive insight into the molecular crosstalk between immunoregulatory mRNAs, miRNAs, and lncRNAs during S. uberis infection. These findings offer valuable directions for the development of molecular diagnosis and biological therapies for mastitis.

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Section: Introductionmentioning

confidence: 99%

Integrated analysis of inflammatory mRNAs, miRNAs, and lncRNAs elucidates the molecular interactome behind bovine mastitis

Hasankhani,

Bakherad,

Bahrami

et al. 2023

Sci Rep

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“…A case of Streptococcus uberis -infection in ducks was also observed. Interestingly, S. uberis is the leading pathogen in dairy herds, causing clinical and subclinical infections ( Tabashiri et al, 2022 ). Moreover, 1 strain of A. anseriformium was isolated from duck liver, and the bacterium has once been isolated from a case of conjunctivitis in duck in 2012 ( Bisgaard and Christensen, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Prevalence and antimicrobial resistance profile of bacterial pathogens isolated from poultry in Jiangxi Province, China from 2020 to 2022

Tan

Yang

et al. 2023

Poultry Science

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“…The regulatory role of miRNAs in response to most mastitis-causing pathogens is not yet well understood. However, discovering the function of miRNAs and their post-transcriptional regulation would aid in understanding the response of the bovine mammary gland to pathogens and improve lactation efficiency, milk quality, and longevity [132,133].…”

Section: Perspectives and Future Challenges For Genomic Genotypic And...mentioning

confidence: 99%

Genomic and Phenotypic Udder Evaluation for Dairy Cattle Selection: A Review

Gutiérrez-Reinoso

Aponte

García-Herreros

2023

Animals

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The traditional point of view regarding dairy cattle selection has been challenged by recent genomic studies indicating that livestock productivity prediction can be redefined based on the evaluation of genomic and phenotypic data. Several studies that included different genomic-derived traits only indicated that interactions among them or even with conventional phenotypic evaluation criteria require further elucidation. Unfortunately, certain genomic and phenotypic-derived traits have been shown to be secondary factors influencing dairy production. Thus, these factors, as well as evaluation criteria, need to be defined. Owing to the variety of genomic and phenotypic udder-derived traits which may affect the modern dairy cow functionality and conformation, a definition of currently important traits in the broad sense is indicated. This is essential for cattle productivity and dairy sustainability. The main objective of the present review is to elucidate the possible relationships among genomic and phenotypic udder evaluation characteristics to define the most relevant traits related to selection for function and conformation in dairy cattle. This review aims to examine the potential impact of various udder-related evaluation criteria on dairy cattle productivity and explore how to mitigate the adverse effects of compromised udder conformation and functionality. Specifically, we will consider the implications for udder health, welfare, longevity, and production-derived traits. Subsequently, we will address several concerns covering the application of genomic and phenotypic evaluation criteria with emphasis on udder-related traits in dairy cattle selection as well as its evolution from origins to the present and future prospects.

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Genome-wide post-transcriptional regulation of bovine mammary gland response to Streptococcus uberis

Cited by 5 publications

References 88 publications

Integrated analysis of inflammatory mRNAs, miRNAs, and lncRNAs elucidates the molecular interactome behind bovine mastitis

Integrated analysis of inflammatory mRNAs, miRNAs, and lncRNAs elucidates the molecular interactome behind bovine mastitis

Prevalence and antimicrobial resistance profile of bacterial pathogens isolated from poultry in Jiangxi Province, China from 2020 to 2022

Genomic and Phenotypic Udder Evaluation for Dairy Cattle Selection: A Review

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