Genome-wide miRNA analysis and integrated network for flavonoid biosynthesis in Osmanthus fragrans

Abstract

Background Osmanthus fragrans is an important economical plant containing multiple secondary metabolites including flavonoids and anthocyanins. During the past years, the roles of miRNAs in regulating the biosynthesis of secondary metabolites in plants have been widely investigated. However, few studies on miRNA expression profiles and the potential roles in regulating flavonoid biosynthesis have been reported in O. fragrans. Results In this study, we used high-throughput sequencing technology to analyze the expression profiles of miRNAs in leaf and flower tissues of O. fragrans. As a result, 106 conserved miRNAs distributed in 47 families and 88 novel miRNAs were identified. Further analysis showed there were 133 miRNAs differentially expressed in leaves and flowers. Additionally, the potential target genes of miRNAs as well as the related metabolic pathways were predicted. In the end, flavonoid content was measured in flower and leaf tissues and potential role of miR858 in regulating flavonoid synthesis was illustrated in O. fragrans. Conclusions This study not only provided the genome-wide miRNA profiles in the flower and leaf tissue of O. fragrans, but also investigated the potential regulatory role of miR858a in flavonoid synthesis in O. fragrans. The results specifically indicated the connection of miRNAs to the regulation of secondary metabolite biosynthesis in non-model economical plant.

Integrative Analysis of miRNAs and Their Targets Involved in Ray Floret Growth in Gerbera hybrida

“…The transcripts per million mapped reads (TPM) of these conserved miRNAs varied from 19.3 (ghy-miR5368) to 330,150 (ghy-miR166e), suggesting that the expression patterns of the various miRNAs are extremely different. Five miRNA families (ghy-miR162, ghy-miR166, ghy-miR319, ghy-miR6113 and ghy-miR6118; comprising 10 individual miRNAs) are highly expressed (TPM ≥ 10,000) at S1, S3 or S6, which is similar to previous results in Carya cathayensis [67] and Osmanthus fragrans [68]. In addition, eight conserved miRNA families, i.e., ghy-miR156, ghy-miR160, ghy-miR164, ghy-miR167, ghy-miR168, ghy-miR171, ghy-miR172 and ghy-miR390, showed moderate expression levels (TPM between 100 and 10,000) at S1, S3 or S6.…”

Involvement of miRNAs in Metabolic Herbicide Resistance to Bispyribac-Sodium in Echinochloa crus-galli (L.) P. Beauv.

“…The miRNAs we considered in this study have previously been identified in other plant species as playing a role in hybridization [ 59 ], growth regulation [ 60 , 61 , 62 , 63 ] and in response to various biotic and abiotic stresses including nutrient deficiency, drought, cold and salinity [ 64 , 65 , 66 , 67 , 68 , 69 , 70 ]. Moreover, miRNAs of the miR396 family are known to target oxidases, including cytochromes involved in xenobiotic detoxification [ 71 ].…”

Integrative analyses of morphology, physiology, and transcriptional expression profiling reveal miRNAs involved in culm color in bamboo

“…We speculated that the differences in the samples and detection methods might be the cause of the diversity in the results. In addition, the processes of culm color deposition are complex, and many regulatory factors (TFs and miRNAs) may participate in the flavonoid synthesis pathway (Shi et al, 2021 ; Lv et al, 2022 ).…”