Genome-Wide Mechanisms of Lifespan Extension by Dietary Restriction in Yeast

Abstract

SUMMARYWe show that the transcription factor Ste12 is needed for full lifespan extension and cell-cycle arrest in response to nutrient limitation; linking the pheromone/invasive growth pathway with cell survivorship. Strikingly, STE12 overexpression was sufficient to extend chronological lifespan under non-restricted conditions. Our global picture of the genetic players of longevity by dietary restriction highlights intricate regulatory cross-talks in aging cells.

A role for cell polarity in lifespan and mitochondrial quality control in the budding yeast Saccharomyces cerevisiae

“…Nonetheless, the bud site machinery is critical for lifespan control in yeast: loss of BUD1 and polarity cueguided bud site selection reduces replicative lifespan (RLS) (Clay et al, 2014) and chronological lifespan (Campos et al, 2018). These studies also revealed that BUD1/RSR1 is required for establishing a diffusion barrier in the ER at the bud neck, which promotes retention of oxidatively damaged ER proteins in the mother cell (Clay et al, 2014).…”

Tra1 controls the transcriptional landscape of the aging cell

“…Since caloric restriction efficiently increases longevity in yeast and other organisms (Jiang et al 2000;Fontana et al 2010;Choi et al 2013;Arlia-Ciommo et al 2014;Leonov et al 2017;Campos et al 2018), we then tested whether functional Tra1 is required for lifespan extension by caloric restriction. Caloric restriction is achieved by growing the cells in 0.1% glucose medium whereas complete medium contains 2% glucose.…”