2010
DOI: 10.2217/epi.10.18
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Genome-Wide Mapping of Histone Modifications and Mass Spectrometry Reveal H4 Acetylation Bias and H3K36 Methylation at Gene Promoters in Fission Yeast

Abstract: Histone modification patterns could be linked to gene expression in fission yeast.

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Cited by 23 publications
(21 citation statements)
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“…We note that increased pho1 + transcription is also evident in expression profiling data from set2Δ cells (47). In addition, pho1 + mRNA levels similarly accumulated in cells lacking the activity of Clr6CII Rpd3S (p st2Δ or alp13Δ ), Spt6 ( spt6-1 ) and FACT ( spt16-18 or pob3Δ ) (Figure 6E, and Supplementary Figure S2).…”
Section: Resultsmentioning
confidence: 66%
“…We note that increased pho1 + transcription is also evident in expression profiling data from set2Δ cells (47). In addition, pho1 + mRNA levels similarly accumulated in cells lacking the activity of Clr6CII Rpd3S (p st2Δ or alp13Δ ), Spt6 ( spt6-1 ) and FACT ( spt16-18 or pob3Δ ) (Figure 6E, and Supplementary Figure S2).…”
Section: Resultsmentioning
confidence: 66%
“…In contrast, there are no homologs of Dot1 or detectable levels of H3K79me in the fission yeast Schizosaccharomyces pombe (Sinha et al 2010). Thus, while gene expression at the heterochromatin of S. pombe is also known to be (D) at ADE2-TEL05R and native TEL06R using the cultures described in B.…”
Section: Discussionmentioning
confidence: 97%
“…Histones were purified following a previously described purification protocol with some modification (Sinha et al, 2010). A 150 mL culture was inoculated to a density of 1.4 × 10 6 cells/mL in 4X YES media from a starter culture grown overnight in 4X YES at 25°C.…”
Section: Methodsmentioning
confidence: 99%
“…Native WCE and chromatin enrichment (NIB buffer) . Cell lysates were fractionated using a method modified from (Ekwall et al, 1997; Sinha et al, 2010). Briefly, cell pellets from 50 ml cultures in YES were washed with NIB [0.25 M sucrose, 60 mM KCl, 15 mM NaCl, 5 mM MgCl 2 , 1 mM CaCl 2 , 2.5 mM spermidine, 20 mM HEPES, 10 mM N-butyric acid, 0.8% Triton X-100 (w/v) with 1:1000 protease inhibitors (Sigma (P8215)) and 1 mM phenylmethylsulfonyl fluoride (PMSF)] and resuspended in 1 ml NIB.…”
Section: Methodsmentioning
confidence: 99%