Genome-Wide Mapping of Adult Plant Resistance to Leaf Rust and Stripe Rust in CIMMYT Wheat Line Arableu#1

Yuan, Chan; Singh, Ravi P.; Liu, Demei; Randhawa, M. S.; Espino, Julio Huerta; Lan, Caixia

doi:10.1094/pdis-10-19-2198-re

Cited by 19 publications

(9 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to different responses to FOV4 among different cotton cultivars, the disease progression caused by FOV4 may follow a sigmoid model if some of the cultivars have resistance genes in response to FOV4 infections during early stages of infections at both 7 and 14 days post-inoculations, as shown by Zhang et al ( 2021b ). It is also a well-known fact that many genes or quantitative trait loci (QTLs) confer adult resistance but not at the seedling stage in cereal crops (Chen, 2013 ; Zhou et al, 2019 ; Yuan et al, 2020 ). Our results showed that the increase in mortality for highly susceptible genotypes like the Pima S-7 cultivar (with ca.…”

Section: Discussionmentioning

confidence: 99%

Studies of Evaluation Methods for Resistance to Fusarium Wilt Race 4 (Fusarium oxysporum f. sp. vasinfectum) in Cotton: Effects of Cultivar, Planting Date, and Inoculum Density on Disease Progression

et al. 2022

View full text Add to dashboard Cite

Fusarium wilt caused by Fusarium oxysporum f. sp. vasinfectum race 4 (FOV4) is an early season disease causing root rot, seedling wilt, and death. To develop an appropriate field evaluation method for resistance to FOV4 in cotton breeding, the objectives of this study were to investigate the effects of cultivar, planting date, and inoculum density on disease progression in 2020–2021. Results showed that the usual local mid-April planting had the lowest disease severity (DSR) or mortality rate (MR) in 2020 and 2021. DSR or MR increased at the late April and early May plantings in both years and reached the highest at the early May planting in 2020, while MR in 2021 was followed by a decrease in the late May planting and reached the highest in the mid-June planting. Local daily low temperatures between mid-April and mid-June were favorable for FOV4 infections, whereas daily high temperatures at 35°C or higher suppressed wilt severity. When seedlings at the 2-true leaf stage were inoculated with 104, 105, 106, and 107 conidia ml−1 per plant in 2020, DSR was low but a linear relationship between inoculum density and DSR was observed. When a FOV4-infested soil supplemented with artificial inoculation was used, disease progression in three moderately susceptible or moderately resistant cultivars followed a linear model, while it followed a quadratic model in the highly susceptible Pima S-7 cultivar only. Among the other three cultivars, FM 2334GLT had the lowest DSR or MR except for one planting date in both years, followed by PHY 725 RF and Pima PHY 881 RF in ascending order, which were consistent with the difference in regression coefficients of the linear models. This study demonstrates that disease progression curves due to FOV4 can be used to compare responses to FOV4 infections among cotton genotypes in cotton breeding and genetic studies, regardless of planting date and inoculation method.

show abstract

Section: Discussionmentioning

confidence: 99%

Studies of Evaluation Methods for Resistance to Fusarium Wilt Race 4 (Fusarium oxysporum f. sp. vasinfectum) in Cotton: Effects of Cultivar, Planting Date, and Inoculum Density on Disease Progression

et al. 2022

View full text Add to dashboard Cite

show abstract

“…These QTL explained 6−13% of the phenotypic variance. Several studies involving biparental mapping populations and genome‐wide association mapping have identified small‐ to medium‐effect inconsistent QTL in CIMMYT wheat germplasm corresponding to the same chromosomes (Basnet et al., 2014; Gao et al., 2016; Lan et al., 2014; Yuan et al., 2020). It is more likely that these four QTL represent the previously reported leaf rust resistance loci in CIMMYT lines.…”

Section: Discussionmentioning

confidence: 99%

“…Association mapping identified genomic regions for leaf rust resistance on chromosomes 2B, 3A, and 5D. In another study, a QTL was reported on chromosome arm 3DS in the CIMMYT line ‘Arableu#1’, which explained up to 4% phenotypic variance (Yuan et al., 2020). Arableu#1 has one parent line common with Copio in their pedigrees.…”

Section: Discussionmentioning

confidence: 99%

“…Molecular marker assay for Lr46 (csLV46G22) was positive in Copio (Table 4). Yuan et al (2020) mapped Lr46 on 1BL spanning the 669.2-673.7 Mb interval. Several other studies have also identified Lr46 in the same genomic region involving different populations, and comparative mapping found 672.6 to 673.8 Mb as the common sequence region (Yuan et al, 2020).…”

Section: Crop Sciencementioning

confidence: 99%

See 1 more Smart Citation

Quantitative trait loci mapping reveals the complexity of adult plant resistance to leaf rust in spring wheat ‘Copio’

et al. 2022

Self Cite

View full text Add to dashboard Cite

The spring wheat (Triticum aestivum L.) line 'Copio' has exhibited high level of adult plant resistance (APR) to the leaf rust (Puccinia triticina) pathogen in Mexico during field evaluations. To elucidate the genetic basis of leaf rust resistance in Copio, 176 F 4 -derived F 6 -recombinant inbred lines (RILs) from a cross of wheat lines 'Apav' and Copio were phenotyped in the field for two seasons in the United States and Mexico. A total of 762 genotyping-by-sequencing (GBS) single nucleotide polymorphic (SNP) markers were used to develop linkage maps. Composite interval mapping identified seven quantitative trait loci (QTL), all contributed by Copio. Three QTL on chromosome arms 1BL (QLr.umn-1B), 2AS (QLr.umn-2A), and 3BS (QLr.umn-3B) were consistently expressed across all four environments. The QTL on 1BL represents Lr46, which is a pleiotropic APR gene, while the QTL on 2AS is colocated to the Lr37 gene in the 2NS/2AS translocation fragment. The QTL on 3BS, mapped to the Sr2/Yr30/Lr27 genomic region, is more likely to be a unique locus conferring APR to leaf rust races because all phenotyping environments had Lr27 virulent pathotypes. Moreover, the functionality of Lr27 is complimented by Lr31 on chromosome 4BS, which is lacking in both parents. Marker haplotypes identified seven RILs carrying a combination of resistance alleles at all three loci. This combination reduced leaf rust coefficient of infection up to 52 and 36% in the Mexican and U.S. environments, respectively. This study reports the complex genetic mechanism of APR to leaf rust in Copio and its importance as a potential resistance source for gene pyramiding through recombination breeding.

show abstract

“…Therefore, we conclude that QYr.cim-1BL/QLr.cim-1BL should be the known APR gene Lr46/Yr29/Sr58/Pm39. Over the last two decades, many CIMMYT derived bread and durum wheat have been reported to possess Lr46/Yr29/Sr58/Pm39: for example, "Pavon 76" (William et al, 2006), "Saar" (Lillemo et al, 2008), "Pastor" (Rosewarne et al, 2012), "Quaiu 3" (Basnet et al, 2013), "Francolin#1" (Lan et al, 2014), "Sujata" (Lan et al, 2015), "Kundan" (Ren et al, 2017), "Bairds" (Lan et al, 2017b), "Chilero" (Ponce-Molina et al, 2018), and Arableu#1 (Yuan et al, 2020). Based on all reported studies, Lr46/Yr29/Sr58/Pm39 should be placed from 662.1 to 684.8 Mb, according to the CS reference genome (International Wheat Genome Sequencing Consortium, 2018).…”

Section: Resistance Loci On Group Chromosomesmentioning

confidence: 99%

Stripe rust and leaf rust resistance in CIMMYT wheat line “Mucuy” is conferred by combinations of race-specific and adult-plant resistance loci

et al. 2022

Self Cite

View full text Add to dashboard Cite

Developing wheat varieties with durable resistance is a core objective of the International Maize and Wheat Improvement Center (CIMMYT) and many other breeding programs worldwide. The CIMMYT advanced wheat line “Mucuy” displayed high levels of resistance to stripe rust (YR) and leaf rust (LR) in field evaluations in Mexico and several other countries. To determine the genetic basis of YR and LR resistance, 138 F5 recombinant inbred lines (RILs) derived from the cross of Apav#1× Mucuy were phenotyped for YR responses from 2015 to 2020 at field sites in India, Kenya, and Mexico, and LR in Mexico. Seedling phenotyping for YR and LR responses was conducted in the greenhouse in Mexico using the same predominant races as in field trials. Using 12,681 polymorphic molecular markers from the DArT, SNP, and SSR genotyping platforms, we constructed genetic linkage maps and QTL analyses that detected seven YR and four LR resistance loci. Among these, a co-located YR/LR resistance loci was identified as Yr29/Lr46, and a seedling stripe rust resistance gene YrMu was mapped on the 2AS/2NS translocation. This fragment also conferred moderate adult plant resistance (APR) under all Mexican field environments and in one season in Kenya. Field trial phenotyping with Lr37-virulent Puccinia triticina races indicated the presence of an APR QTL accounting for 18.3–25.5% of the LR severity variation, in addition to a novel YR resistance QTL, QYr.cim-3DS, derived from Mucuy. We developed breeder-friendly KASP and indel molecular markers respectively for Yr29/Lr46 and YrMu. The current study validated the presence of known genes and identified new resistance loci, a QTL combination effect, and flanking markers to facilitate accelerated breeding for genetically complex, durable rust resistance.

show abstract

Genome-Wide Mapping of Adult Plant Resistance to Leaf Rust and Stripe Rust in CIMMYT Wheat Line Arableu#1

Cited by 19 publications

References 69 publications

Studies of Evaluation Methods for Resistance to Fusarium Wilt Race 4 (Fusarium oxysporum f. sp. vasinfectum) in Cotton: Effects of Cultivar, Planting Date, and Inoculum Density on Disease Progression

Studies of Evaluation Methods for Resistance to Fusarium Wilt Race 4 (Fusarium oxysporum f. sp. vasinfectum) in Cotton: Effects of Cultivar, Planting Date, and Inoculum Density on Disease Progression

Quantitative trait loci mapping reveals the complexity of adult plant resistance to leaf rust in spring wheat ‘Copio’

Stripe rust and leaf rust resistance in CIMMYT wheat line “Mucuy” is conferred by combinations of race-specific and adult-plant resistance loci

Contact Info

Product

Resources

About