Genome-wide identification of wheat (Triticum aestivum) expansins and expansin expression analysis in cold-tolerant and cold-sensitive wheat cultivars

Zhang, Junfeng; Xu, Yongqing; Dong, J. Q.; Peng, Lina; Xu, Feng; Wang, Xu; Li, Fēi; Yu, Miao; ShuKuan, Yao; Zhao, Qing Shan; Feng, Shanshan; Bao-zhong, HU

doi:10.1371/journal.pone.0195138

Cited by 25 publications

(33 citation statements)

References 62 publications

(93 reference statements)

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“…Since expansin proteins were first described two decades ago, many expansin genes have been identified in genomic studies of various plant species, including tomato, maize and tobacco (Zhang et al, ; Ding et al, ; Lu et al, ). In a previous study, we identified 128 expansin genes in a genome and an ancestral genome (Zhang et al, ). Here, the T. aestivum expansin superfamily was found to include 102 EXPA genes, 75 EXPB genes and 15 EXLA genes; no EXLB genes were detected (Table S4, Figure S2).…”

Section: Discussionmentioning

confidence: 99%

“…Clean reads from the nine transcriptomes were filtered to remove low‐quality reads (reads with a Q ‐value ≤ 20). The remaining clean reads were analysed using the T. aestivum genome (ftp://ftp.ensemblgenfmes.org/pub/release32/plants/fasta/triticum_aestivum/; Zhang et al, ) as reference. Gene expression levels were calculated using the fragments per kilobase per million fragments mapped (FPKM) method (Livak & Schmittgen ).…”

Section: Methodsmentioning

confidence: 99%

“…The gene annotation files and protein data for the T. aestivum genome were downloaded from the T. aestivum genome website (ftp://ftp.ensemblgenfmes.org/pub/release32/plants/fasta/triticum_aestivum/; Zhang et al, ). The hidden Markov models (HMM) for DPBB_1 PF03330 and Pollen_allerg_1 PF01357 were downloaded from Pfam (http://pfam.sanger.ac.uk/).…”

Section: Methodsmentioning

confidence: 99%

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Overexpression of paralogues of the wheat expansin gene TaEXPA8 improves low‐temperature tolerance in Arabidopsis

Peng

Wang

et al. 2019

Plant Biol J

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Low temperature is one of the important factors limiting wheat yield in cold regions.Expansins are nonenzymatic proteins that loosen cell walls and play important roles in diverse biological processes related to cell wall modification, including development and stress tolerance. Many studies have shown that expansins are involved in resistance to various abiotic stresses, such as heat and drought. However, the role of expansins in response to low-temperature stress remains unclear.• Based on our previous transcriptome data of a winter wheat cultivar Dongnongdongmai 2 (DN2), we found that one of the expansin genes, TaEXPA8, was significantly induced by low temperature, indicating a role for TaEXPA8 in cold resistance. In this study, the paralogous TaEXPA8 genes TaEXPA8-A, TaEXPA8-B and TaEXPA8-D were cloned by RT-PCR. These three genes were then transformed into Arabidopsis by the floral dip method. Expression patterns of TaEXPA8 genes in different tissues and in response to several abiotic stresses and hormones were detected by quantitative realtime PCR (qRT-PCR).• The results showed that TaEXPA8-A and TaEXPA8-B were expressed mainly in roots, while TaEXPA8-D was expressed predominantly in flowers. TaEXPA8 genes were induced by low-temperature and drought. The overexpression of TaEXPA8-B and TaEXPA8-D enhanced low-temperature resistance and had increased superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activity and soluble protein, MDA and proline content.• In summary, our study suggested that the expansins TaEXPA8-B and TaEXPA8-D are involved in the response to low temperature and possibly play a role in cold resistance by activating the protective enzyme system.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Overexpression of paralogues of the wheat expansin gene TaEXPA8 improves low‐temperature tolerance in Arabidopsis

Peng

Wang

et al. 2019

Plant Biol J

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“…Thell] (2n = 4x = 28; AABB), which subsequently hybridized with Aegilops tauschii (D-genome donor) to form the current genome composition of bread wheat (2n = 6x = 42; AABBDD) [ 38 ]. These events have modified the number and/or levels of expression of different gene families, such as EXP expansins, SWEET sugar transporters, MAPK and MAPKK kinases, PHT1 phosphate transporters, among others, implicating a change in their biological role [ 39 , 40 , 41 , 42 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Transcriptional Regulation of Aquaporin Genes in Bread Wheat (Triticum aestivum L.) under Water Stress

Madrid-Espinoza

Brunel-Saldias

Guerra

et al. 2018

Genes

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Aquaporins (AQPs) are transmembrane proteins essential for controlling the flow of water and other molecules required for development and stress tolerance in plants, including important crop species such as wheat (Triticum aestivum). In this study, we utilized a genomic approach for analyzing the information about AQPs available in public databases to characterize their structure and function. Furthermore, we validated the expression of a suite of AQP genes, at the transcriptional level, including accessions with contrasting responses to drought, different organs and water stress levels. We found 65 new AQP genes, from which 60% are copies expanded by polyploidization. Sequence analysis of the AQP genes showed that the purifying selection pressure acted on duplicate genes, which was related to a high conservation of the functions. This situation contrasted with the expression patterns observed for different organs, developmental stages or genotypes under water deficit conditions, which indicated functional divergence at transcription. Expression analyses on contrasting genotypes showed high gene transcription from Tonoplast Intrinsic Protein 1 (TIP1) and 2 (TIP2), and Plasma Membrane Intrinsic Protein 1 (PIP1) and 2 (PIP2) subfamilies in roots and from TIP1 and PIP1 subfamilies in leaves. Interestingly, during severe drought stress, 4 TIP genes analyzed in leaves of the tolerant accession reached up to 15-fold the level observed at the susceptible genotype, suggesting a positive relationship with drought tolerance. The obtained results extend our understanding of the structure and function of AQPs, particularly under water stress conditions.

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“…In wheat, the transcript of TaExpA6 was found in the pericarp during early growth in grain development and, subsequently, in both endosperm and pericarp (LIZANA et al, 2010). Meanwhile, the expression of expansin genes were affected by various environmental stresses such as oxidative stress (CHEN et al, 2018), cold stress (ZHANG et al, 2018), salinity stress (CHEN et al, 2017), drought stress (CHEN et al, 2016) and cadmium toxicity stress (REN et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Expression and function analysis of wheat expasin genes EXPA2 and EXPB1

et al. 2019

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Expansins are a group of plant cell wall loosening proteins that play important roles in plant growth and development. In this study, we performed the first study on the molecular characterization, transcriptional expression and functional properties of two wheat expansin genes TaEXPA2 and TaEXPB1. The results indicated that TaEXPA2 and TaEXPB1 genes had typical structural features of plant expansin gene family. As a member of α-expansins, TaEXPA2 is closely related to rice OsEXPA17 while the βexpansin member TaEXPB1 has closely phylogenetic relationships with rice OsEXPAB4. The genetic transformation to Arabidopsis showed that both TaEXPA2 and TaEXPB1 were located in cell wall and highly expressed in roots, leaves and seeds. Overexpression of TaEXPA2 and TaEXPB1 genes showed similar functions, causing rapid root elongation, early bolting, and increases in leaves number, rosette diameter and stems length. These results demonstrated that wheat expansin genes TaEXPA1 and TaEXPB2 can enhance plant growth and development.

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Genome-wide identification of wheat (Triticum aestivum) expansins and expansin expression analysis in cold-tolerant and cold-sensitive wheat cultivars

Cited by 25 publications

References 62 publications

Overexpression of paralogues of the wheat expansin gene TaEXPA8 improves low‐temperature tolerance in Arabidopsis

Overexpression of paralogues of the wheat expansin gene TaEXPA8 improves low‐temperature tolerance in Arabidopsis

Genome-Wide Identification and Transcriptional Regulation of Aquaporin Genes in Bread Wheat (Triticum aestivum L.) under Water Stress

Expression and function analysis of wheat expasin genes EXPA2 and EXPB1

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