Genome-wide identification of sweet orange ( Citrus sinensis ) metal tolerance proteins and analysis of their expression patterns under zinc, manganese, copper, and cadmium toxicity

Fu, Xing-Zheng; Tong, Ya-Hua; Zhou, Xue; Li, Lili; ChangPin, Chun; Cao, Li; Zeng, Ming; LiangZhi, Peng

doi:10.1016/j.gene.2017.07.072

Cited by 50 publications

(61 citation statements)

References 35 publications

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“…Additionally, although Cu has not been identified as substrates for MTP transporters even in model plants, we found that Cu induced the expression levels of most BrrMTP genes. The results are supported by a recent study in sweet orange ( Fu et al, 2017 ); thus these findings provide valuable information for future studies on the role of MTPs in Cu detoxification. Nevertheless, we also found that some BrrMTP genes responded to Mg and Na, which have not been used as substrates for MTP transporters in plants either ( Kolaj-Robin et al, 2015 ).…”

Section: Discussionsupporting

confidence: 84%

“…On the basis of the increasing genome information, analyzing gene families via comparative genomics is an efficient method for modern functional genomics research ( Xu et al, 2016 ). However, unlike many other gene families, including some transcription factors and kinases ( Iftikhar et al, 2017 ; Ma et al, 2017 ; Xi et al, 2017 ), studies on the characteristics and functional analysis of the plant MTP gene family are just reported in few species recently ( Fu et al, 2017 ; Vatansever et al, 2017 ). As MTP genes have been demonstrated to participate in tolerating and transporting various heavy metals, including plant trace elements and non-essential elements ( Kim et al, 2004 ; Delhaize et al, 2007 ; Xu et al, 2009 ; Lang et al, 2011 ), they might thus play significant roles in plant mineral nutrition maintenance and resistance to stresses caused by metals.…”

Section: Discussionmentioning

confidence: 99%

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Genome-Wide Identification and Expression Analysis of the Cation Diffusion Facilitator Gene Family in Turnip Under Diverse Metal Ion Stresses

et al. 2018

Front. Genet.

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The cation diffusion facilitator (CDF) family is one of the gene families involved in metal ion uptake and transport in plants, but the understanding of the definite roles and mechanisms of most CDF genes remain limited. In the present study, we identified 18 candidate CDF genes from the turnip genome and named them BrrMTP1.1–BrrMTP12. Then, we performed a comparative genomic analysis on the phylogenetic relationships, gene structures and chromosome distributions, conserved domains, and motifs of turnip CDFs. The constructed phylogenetic tree indicated that the BrrMTPs were divided into seven groups (groups 1, 5, 6, 7, 8, 9, and 12) and formed three major clusters (Zn-CDFs, Fe/Zn-CDFs, and Mn-CDFs). Moreover, the structural characteristics of the BrrMTP members in the same group were similar but varied among groups. To investigate the potential roles of BrrMTPs in turnip, we conducted an expression analysis on all BrrMTP genes under Mg, Zn, Cu, Mn, Fe, Co, Na, and Cd stresses. Results showed that the expression levels of all BrrMTP members were induced by at least one metal ion, indicating that these genes may be related to the tolerance or transport of those metal ions. Based on the roles of different metal ions for plants, we hypothesized that BrrMTP genes are possibly involved in heavy metal accumulation and tolerance to salt stress apart from their roles in the maintenance of mineral nutrient homeostasis in turnip. These findings are helpful to understand the roles of MTPs in plants and provide preliminary information for the study of the functions of BrrMTP genes.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of the Cation Diffusion Facilitator Gene Family in Turnip Under Diverse Metal Ion Stresses

et al. 2018

Front. Genet.

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“…For instance, excess Cu, which is not a potential substrate for the MTP family, could induce the transcription levels of PtrMTP genes (Figure 7, Table 5). Similar findings were also made in sweet orange and turnip [24,25]. Other than Mn, the expression of the Mn-CDFs, PtrMTP9 and PtrMTP10.3 were sharply upregulated by excess Fe and Zn, respectively (Figure 7, Table 5).…”

Section: The Diverse Expression Patterns Of Ptrmtp Genessupporting

confidence: 80%

“…Compared with that of previous report, three new MTP genesn were found in P. trichocarpa [20]. The number of MTPs in P. trichocarpa was only second to N. tabacum among the plant species in which the MTPs have been identified [5,8,[22][23][24][25][26][27][28]. However, despite the large number of PtrMTPs, the homolog of AtMTP2 in P. trichocarpa was not detected.…”

Section: Evolution and Differentiation Of Ptrmtp Genes As Well As Thecontrasting

confidence: 61%

Genome-Wide Identification of Metal Tolerance Protein Genes in Populus trichocarpa and Their Roles in Response to Various Heavy Metal Stresses

Yang

Liu

et al. 2020

IJMS

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Metal tolerance proteins (MTPs) are plant divalent cation transporters that play important roles in plant metal tolerance and homeostasis. Poplar is an ideal candidate for the phytoremediation of heavy metals because of its numerous beneficial attributes. However, the definitive phylogeny and heavy metal transport mechanisms of the MTP family in poplar remain unknown. Here, 22 MTP genes in P. trichocarpa were identified and classified into three major clusters and seven groups according to phylogenetic relationships. An evolutionary analysis suggested that PtrMTP genes had undergone gene expansion through tandem or segmental duplication events. Moreover, all PtrMTPs were predicted to localize in the vacuole and/or cell membrane, and contained typical structural features of the MTP family, cation efflux domain. The temporal and spatial expression pattern analysis results indicated the involvement of PtrMTP genes in poplar developmental control. Under heavy metal stress, most of PtrMTP genes were induced by at least two metal ions in roots, stems or leaves. In addition, PtrMTP8.1, PtrMTP9 and PtrMTP10.4 displayed the ability of Mn transport in yeast cells, and PtrMTP6 could transport Co, Fe and Mn. These findings will provide an important foundation to elucidate the biological functions of PtrMTP genes, and especially their role in regulating heavy metal tolerance in poplar.

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“…The seeds of "Hamlin" sweet orange were collected from the Citrus Research Institute, Southwest University, Chongqing, China. The seeds were sterilized and germinated, and the seedlings were hydroponically cultured at 25 • C and 16 h photoperiod (50 µmol m −2 s −1 ), as described by Fu et al [41]. After 30 days of growth in hydroponic solution, uniform seedlings were divided into different groups (3 biological repeats and 10 plants per repeat for each group) for the following sets of treatments.…”

Section: Plant Materials and Treatmentsmentioning

confidence: 99%

Comprehensive Analysis of Autophagy-Related Genes in Sweet Orange (Citrus sinensis) Highlights Their Roles in Response to Abiotic Stresses

Zhou

et al. 2020

IJMS

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Autophagy is a highly conserved intracellular degradation pathway that breaks down damaged macromolecules and/or organelles. It is involved in plant development and senescence, as well as in biotic and abiotic stresses. However, the autophagy process and related genes are largely unknown in citrus. In this study, we identified 35 autophagy-related genes (CsATGs—autophagy-related genes (ATGs) of Citrus sinensis, Cs) in a genome-wide manner from sweet orange (Citrus sinensis). Bioinformatic analysis showed that these CsATGs were highly similar to Arabidopsis ATGs in both sequence and phylogeny. All the CsATGs were randomly distributed on nine known (28 genes) and one unknown (7 genes) chromosomes. Ten CsATGs were predicted to be segmental duplications. Expression patterns suggested that most of the CsATG were significantly up- or down-regulated in response to drought; cold; heat; salt; mannitol; and excess manganese, copper, and cadmium stresses. In addition, two ATG18 members, CsATG18a and CsATG18b, were cloned from sweet orange and ectopically expressed in Arabidopsis. The CsATG18a and CsATG18b transgenic plants showed enhanced tolerance to osmotic stress, salt, as well as drought (CsATG18a) or cold (CsATG18b), compared to wild-type plants. These results highlight the essential roles of CsATG genes in abiotic stresses.

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Genome-wide identification of sweet orange ( Citrus sinensis ) metal tolerance proteins and analysis of their expression patterns under zinc, manganese, copper, and cadmium toxicity

Cited by 50 publications

References 35 publications

Genome-Wide Identification and Expression Analysis of the Cation Diffusion Facilitator Gene Family in Turnip Under Diverse Metal Ion Stresses

Genome-Wide Identification and Expression Analysis of the Cation Diffusion Facilitator Gene Family in Turnip Under Diverse Metal Ion Stresses

Genome-Wide Identification of Metal Tolerance Protein Genes in Populus trichocarpa and Their Roles in Response to Various Heavy Metal Stresses

Comprehensive Analysis of Autophagy-Related Genes in Sweet Orange (Citrus sinensis) Highlights Their Roles in Response to Abiotic Stresses

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