Genome-wide identification of small heat shock protein (HSP20) homologs in three cucurbit species and the expression profiles of CsHSP20s under several abiotic stresses

Xiao, Wang; Zheng, Yujie; Chen, Birong; Zhi, Chengchen; Qiao, Liang; Liu, Ce; Pan, Yupeng; Cheng, Zhihui

doi:10.1016/j.ijbiomac.2021.08.222

Cited by 18 publications

(11 citation statements)

References 43 publications

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“…This was also observed in other plants, for example, apple and soybean [13,28]. In this study, the HSP20s of peach lacked any proteins in the CVII subgroup, similar to soybean [28], rice [18], switchgrass [34], apple [13], and three cucurbit species (cucumber, melon, and watermelon) [14]. In Arabidopsis thaliana, the CVII subgroup gene AtHsp14.7 was constitutively expressed, and its transcript level did not change under different stresses [40].…”

Section: Discussionsupporting

confidence: 66%

“…42 in Solanum lycopersicum [20], 63 in Panicum virgatum [34], 48 in Solanum tuberosum [35], 48 in Vitis vinifera [22], 47 in Sorghum bicolor [23], 41 in Malus pumila [13], 47 in Cucumis sativus [14], 45 in Cucumis melo [14], and 47 in Citrullus lanatus [14]. In peach, we identified 42 PpHSP20s, a number greater than in Arabidopsis thaliana and pepper, but lower than in switchgrass, potato, and grape.…”

Section: Discussionmentioning

confidence: 85%

“…Tremendous amounts of research have shown that multiple heat shock proteins (HSPs) emerge as central players in the temperature response, including the growth/development and stress responses [11][12][13][14]. According to their molecular weight, HSPs are divided into five major categories, including HSP100s, HSP90s, HSP70s, HSP60s, and HSP20s [4].…”

Section: Introductionmentioning

confidence: 99%

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Phylogenetic and Transcriptional Analyses of the HSP20 Gene Family in Peach Revealed That PpHSP20-32 Is Involved in Plant Height and Heat Tolerance

Lian

Wang

et al. 2022

IJMS

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The heat shock protein 20 (HSP20) proteins comprise an ancient, diverse, and crucial family of proteins that exists in all organisms. As a family, the HSP20s play an obvious role in thermotolerance, but little is known about their molecular functions in addition to heat acclimation. In this study, 42 PpHSP20 genes were detected in the peach genome and were randomly distributed onto the eight chromosomes. The primary modes of gene duplication of the PpHSP20s were dispersed gene duplication (DSD) and tandem duplication (TD). PpHSP20s in the same class shared similar motifs. Based on phylogenetic analysis of HSP20s in peach, Arabidopsis thaliana, Glycine max, and Oryza sativa, the PpHSP20s were classified into 11 subclasses, except for two unclassified PpHSP20s. cis-elements related to stress and hormone responses were detected in the promoter regions of most PpHSP20s. Gene expression analysis of 42 PpHSP20 genes revealed that the expression pattern of PpHSP20-32 was highly consistent with shoot length changes in the cultivar ‘Zhongyoutao 14’, which is a temperature-sensitive semi-dwarf. PpHSP20-32 was selected for further functional analysis. The plant heights of three transgenic Arabidopsis lines overexpressing PpHSP20-32 were significantly higher than WT, although there was no significant difference in the number of nodes. In addition, the seeds of three over-expressing lines of PpHSP20-32 treated with high temperature showed enhanced thermotolerance. These results provide a foundation for the functional characterization of PpHSP20 genes and their potential use in the growth and development of peach.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

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Phylogenetic and Transcriptional Analyses of the HSP20 Gene Family in Peach Revealed That PpHSP20-32 Is Involved in Plant Height and Heat Tolerance

Lian

Wang

et al. 2022

IJMS

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“…Cis- regulatory elements are important molecular switches involved in the transcriptional regulation of gene expression and control various biological processes, including stress responses, hormone responses, and developmental processes ( Yamaguchi-Shinozaki and Shinozaki, 2005 ). Cis- regulatory element predictions have been widely used to explore the functions of Hsp20s in several species ( Wang et al, 2021 ). In our study, various stress-responsive, phytohormone-responsive, light-responsive, and plant development-related cis- elements were found in the promoter regions of red algal Hsp20 genes ( Figure 6 ).…”

Section: Discussionmentioning

confidence: 99%

Heat Shock Protein 20 Gene Superfamilies in Red Algae: Evolutionary and Functional Diversities

Gao

Tang

et al. 2022

Front. Plant Sci.

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Heat shock protein 20 (Hsp20) genes play important roles in plant growth, development, and response to environmental stress. However, the Hsp20 gene family has not yet been systematically investigated, and its function in red algae (Rhodophyta) remains poorly understood. Herein, we characterized Hsp20 gene families in red algae by studying gene structure, conserved motifs, phylogenetic relationships, chromosome location, gene duplication, cis-regulatory elements, and expression profiles. In this study, 97 Hsp20 genes were identified using bioinformatic methods and classified into 13 subfamilies based on phylogenetic relationships. Phylogenetic analysis revealed that Hsp20 genes might have a polyphyletic origin and a complex evolutionary pattern. Gene structure analysis revealed that most Hsp20 genes possessed no introns, and all Hsp20 genes contained a conserved α-crystalline domain in the C-terminal region. Conserved motif analysis revealed that Hsp20 genes belonging to the same subfamily shared similar motifs. Gene duplication analysis demonstrated that tandem and segmental duplication events occurred in these gene families. Additionally, these gene families in red algae might have experienced strong purifying selection pressure during evolution, and Hsp20 genes in Pyropia yezoensis, Pyropia haitanensis, and Porphyra umbilicalis were highly evolutionarily conserved. The cis-elements of phytohormone-, light-, stress-responsive, and development-related were identified in the red algal Hsp20 gene promoter sequences. Finally, using Py. yezoensis, as a representative of red algae, the Hsp20 gene expression profile was explored. Based on the RNA-seq data, Py. yezoensis Hsp20 (PyyHsp20) genes were found to be involved in Py. yezoensis responses against abiotic and biotic stresses and exhibited diverse expression patterns. Moreover, PyyHsp20 is involved in Py. yezoensis growth and development and revealed spatial and temporal expression patterns. These results provide comprehensive and valuable information on Hsp20 gene families in red algae and lay a foundation for their functional characterization. In addition, our study provides new insights into the evolution of Hsp20 gene families in red algae and will help understand the adaptability of red algae to diverse environments.

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“…During the evolution of plant genome and genetic systems, gene duplications have been one of the leading causes for the expansion of gene families (Cannon et al, 2004;Wang et al, 2021;Lin et al, 2022). To further understand the evolution of ANKTM genes in wheat and T. dicoccoides, the tandem duplications of the common wheat, T. dicoccoides and T. urartu of the ANKTM family were investigated.…”

Section: Chromosome Distribution and Gene Duplicationmentioning

confidence: 99%

Identification of ankyrin-transmembrane-type subfamily genes in Triticeae species reveals TaANKTM2A-5 regulates powdery mildew resistance in wheat

Ren

et al. 2022

Front. Plant Sci.

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The ankyrin-transmembrane (ANKTM) subfamily is the most abundant subgroup of the ANK superfamily, with critical roles in pathogen defense. However, the function of ANKTM proteins in wheat immunity remains largely unexplored. Here, a total of 381 ANKTMs were identified from five Triticeae species and Arabidopsis, constituting five classes. Among them, class a only contains proteins from Triticeae species and the number of ANKTM in class a of wheat is significantly larger than expected, even after consideration of the ploidy level. Tandem duplication analysis of ANKTM indicates that Triticum urartu, Triticum dicoccoides and wheat all had experienced tandem duplication events which in wheat-produced ANKTM genes all clustered in class a. The above suggests that not only did the genome polyploidization result in the increase of ANKTM gene number, but that tandem duplication is also a mechanism for the expansion of this subfamily. Micro-collinearity analysis of Triticeae ANKTMs indicates that some ANKTM type genes evolved into other types of ANKs in the evolution process. Public RNA-seq data showed that most of the genes in class d and class e are expressed, and some of them show differential responses to biotic stresses. Furthermore, qRT-PCR results showed that some ANKTMs in class d and class e responded to powdery mildew. Silencing of TaANKTM2A-5 by barley stripe mosaic virus-induced gene silencing compromised powdery mildew resistance in common wheat Bainongaikang58. Findings in this study not only help to understand the evolutionary process of ANKTM genes, but also form the basis for exploring disease resistance genes in the ANKTM gene family.

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Genome-wide identification of small heat shock protein (HSP20) homologs in three cucurbit species and the expression profiles of CsHSP20s under several abiotic stresses

Cited by 18 publications

References 43 publications

Phylogenetic and Transcriptional Analyses of the HSP20 Gene Family in Peach Revealed That PpHSP20-32 Is Involved in Plant Height and Heat Tolerance

Phylogenetic and Transcriptional Analyses of the HSP20 Gene Family in Peach Revealed That PpHSP20-32 Is Involved in Plant Height and Heat Tolerance

Heat Shock Protein 20 Gene Superfamilies in Red Algae: Evolutionary and Functional Diversities

Identification of ankyrin-transmembrane-type subfamily genes in Triticeae species reveals TaANKTM2A-5 regulates powdery mildew resistance in wheat

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